About our lab:
Our lab is addressing the interaction between phytopathogens and host plants with a multidisciplinary perspective.
We are currently working on the following projects:
實驗室研究方向
本實驗室的專長是利用細胞生物學及生物化學等方法，探討植物細菌致病及植物免疫機制。
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Resolve the role of ACTIN against different bacterial pathogens from a spatial perspective
Plants have employed sophisticated immune defense systems to protect themselves from sorts of pathogens. While significant progress has been made in understanding leaf immunity, knowledge about root immunity remains limited. In our lab, we aim to elucidate ACTIN's role in leaf and root immunity. We are using comparative studies to investigate subcellular activities, defense gene activation, and receptor protein kinase signaling in response to Pst DC3000 and R. solanacearum infections. Additionally, we will explore the function of ACTIN in tomato plants through gene overexpression and deletion experiments. By integrating these findings, we anticipate advancing our understanding of ACTIN's role in plant immunity and translating this knowledge to enhance tomato resistance against bacterial pathogens.
探討肌動蛋白於不同細菌性抗性中所扮演的角色
於細胞生物學層次，本實驗室探討植物細菌如何藉由影響細胞內的胞器及構造而致病。例如，先前的研究成果顯示，植物在受細菌的侵染過程中，其細胞骨架-肌動蛋白（Actin）會產生變化，而此變化是細菌藉由第三型分泌系統釋出的HopG1效應子所導致，我們將會基於以上研究成果，探討其中牽涉的訊息傳導路徑。另外，我們將利用分別由葉片及植物根部入侵之病原細菌如 Pst DC3000及 R. solanacearum 感染植物，並探討肌動蛋白在各種植物器官中所呈現之免疫功能及機制。
Decoding Strawberry Angular Leaf Spot Resistance Through Multifaceted Strategies
Decoding Strawberry Angular Leaf Spot Resistance Through Multifaceted Strategies
Strawberry angular leaf spot, caused by Xanthomonas fragariae, is a disease that crashes strawberry yield and economic value in Taiwan. This project aims to identify disease-resistant strawberry varieties and explore sustainable control measures. To accelerate our understanding of strawberry resistance, we will compare the mRNA profiles between strawberry cultivars demonstrating the best and most susceptible phenotypes. Additionally, efforts to replace copper-based formulations with environmentally friendly alternatives have identified small molecules as potential inhibitors of X. fragariae growth. Transcriptomic analysis will be employed to elucidate the underlying inhibitory mechanisms. Given the significance of strawberries as a important crop in Taiwan, this study aims to advance disease-resistant breeding programs, understand the biology of the two X. fragariae isolates, decipher the phyllosphere microbiome community, and promote the use of eco-friendly materials. These efforts aim to reduce reliance on heavy metal-based agents and foster sustainable agricultural practices.
草莓角斑病防禦機制
本實驗室針對造成臺灣嚴重經濟損失之細菌性病害，利用改善資材如使用小分子物質或是微生物防治的方式減少對農藥的依賴性，降低植物細菌對現行用藥產生抗藥性之顧慮，進而營造環境友善之農業管理。以草莓角斑病為例，在該病害爆發期間，對草莓產業造成重大損失。我們將結合分析抗病品種及轉錄體研究，找出相關重要抗病基因，以利後續應用。另外，我們也將探索非農業藥劑之物質如何增強植物防禦的機制，並探討施用小分子物質作為防治資材時，對周遭微生物環境之影響。
Investigation of the role of NDR1 and NHL protein family in plant immunity
Investigation of the role of NDR1 and NHL protein family in plant immunity
The Arabidopsis NDR1 was identified about twenty years ago. It is plasma membrane-localized and functions as a guardee protein that protects RIN4 from degradation. Functional studies demonstrated that NDR1 is involved in multiple pathways during immune signaling. In combination with our preliminary data and protein interaction profiles, we would like to address how NDR1 reacts in each signaling node. The paralogs and orthologs of NDR1 exist widely among all kinds of plants. Some of them share similar sequence features with NDR1 and are named NHL (NDR1/HIN1-like proteins). NHLs broadly regulate plants in reaction to biotic and abiotic stresses. We would like to study and examine the role of NDR1 and NHL, expecting to provide novel perspectives on their roles in plant immunity.
免疫蛋白之訊息傳導
NDR1蛋白位於細胞膜，其調控的機制在免疫反應中可以保護重要抗性蛋白RIN4，避免其遭受分解。與其結構相似的同源體如 NHL (NDR1/HIN1-like proteins) 也廣泛分布在植物中，且也調控植物在生物及環境逆境下之反應。根據之前的研究基礎，我們將解析NDR1與NHLs在免疫訊息中所扮演的功能。
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