Dr. Huan-Cheng Chang - Measuring Nanoscale Thermostability of Cell Membranes with Single Gold-Diam

Measuring Nanoscale Thermostability of Cell Membranes with Single Gold-Diamond Nanohybrids

Pei-Chang Tsai,^1,† Chandra P. Epperla,^1,2,3,† Jo-Shan Huang,⁴ Oliver Y. Chen,¹ Chih-Che Wu,⁴ and Huan-Cheng Chang*^,1,2,5

¹Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106, Taiwan

²Taiwan International Graduate Program – Molecular Science and Technology, Academia Sinica, Taipei 115, Taiwan

³Department of Chemistry, National Tsing Hua University, Hsinchu 300, Taiwan

⁴Department of Applied Chemistry, National Chi Nan University, Puli, Nantou 545, Taiwan

⁵Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan

^†These two authors contributed equally to this work

Angew. Chem. Int. Ed. 56, 3025–3030 (2017).

Much of the current understanding of thermal effects in biological systems is based on macroscopic measurements. There is little knowledge about the local thermostability or heat tolerance of subcellular components at the nanoscale. Herein, we show that gold nanorod-fluorescent nanodiamond (GNR-FND) hybrids are useful as a combined nanoheater-nanothermometer in living cells. With the use of a 594 nm laser for both heating and probing, we measured the temperature changes by recording the spectral shifts of the zero-phonon lines of negatively charged nitrogen-vacancy centers in FNDs. The technique allows us to determine the rupture temperatures of individual membrane nanotubes in human embryonic kidney cells, as well as to generate high temperature gradients on the cell membrane for photoporation and optically controlled hyperthermia. Our results demonstrate a new paradigm for hyperthermia research and application. [link]

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