Dr. Huan-Cheng Chang - Fluorescent Nanodiamond: A Versatile Tool for Long-Term Cell Tracking, Supe

Fluorescent Nanodiamond: A Versatile Tool for Long-Term Cell Tracking, Super-Resolution Imaging, and Nanoscale Temperature Sensing

Wesley Wei-Wen Hsiao,¹ Yuen Yung Hui,¹ Pei-Chang Tsai,¹ and Huan-Cheng Chang*^,1,2

¹Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106, Taiwan

²Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan

Acc. Chem. Res. 49, 400–407 (2016).

Fluorescent nanodiamond (FND) has recently played a central role in fueling new discoveries in interdisciplinary fields spanning biology, chemistry, physics, and materials sciences. The nanoparticle is unique in that it contains a high density ensemble of negatively charged nitrogen-vacancy (NV^–) centers with outstanding optical and magnetic properties. Firstly, NV^– has an absorption maximum at ~550 nm and when exposed to green-orange light, it emits bright fluorescence at ~700 nm with a lifetime of longer than 10 ns. These spectroscopic properties are little affected by surface modification and allow background-free imaging of FNDs in tissue sections. Next, as an artificial atom in the solid state, the NV^– center is perfectly photostable, without photobleaching and blinking. Therefore, the NV-containing FND is suitable as a contrast agent for super-resolution imaging by stimulated emission depletion (STED). Last, the NV^– center in diamond is an atom-like quantum system with a total electron spin of 1 and the ground states of the spins show a crystal field splitting of 2.87 GHz, separating the m_s = 0 and ±1 sublevels. Nanothermometry with both high spatial and temporal resolution can be achieved with a technique known as optically detected magnetic resonance (ODMR). This account provides a summary of the recent advances in FND-enabled technologies with a special focus on long-term cell tracking, super-resolution imaging, and nanoscale temperature sensing. These emerging and multifaceted technologies are in synchronicity with modern imaging modalities. [Fullfile]

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