We are actively pursuing the development of In-situ NMR methods to investigate membrane proteins and transthyretin-related familial amyloid polyneuropathy (TTR-FAP).. 

 

Publications

1. Chang, C.-k.; Wu, Y.-S.; Wu, P.-H.; Li, S.-S.; Yu, T.-Y.; Chen, C.-W. Chemical Profiling and Bottle-Aging Analysis of Sorghum Liquor Unveiled by Nuclear Magnetic Resonance Spectroscopy. J. Chin. Chem. Soc. 2026, 0, 1–12.

2. Chang, H.-C.; Kuo, L.; Yu, T.-Y.; Liu, Y.-T.; Lin, K.-P.; Yu, W.-C. Multidisciplinary Approaches for Diagnosing Underrecognized Transthyretin Amyloidosis in Real-World Practice. Acta Cardiol. Sin. 2026, 42 (2), 216.

3. Shih, O.; Feng, Y.-C.; Agrawal, S.; Liao, K.-F.; Yeh, Y.-Q.; Chang, J.-W.; Yu, T.-Y.; Jeng, U.-S. Differentiating the Solution Structures and Stability of Transthyretin Tetramer Complexed with Tolcapone and Tafamidis Using SEC-SWAXS and NMR. J. Appl. Crystallogr. 2025, 58 (4), 1373–1383.

4. Wu, Y.-S.; Chu, L.-K.; Yu, T.-Y. Investigation of Methionine Metabolism in Coccolithophore by In Situ Light-Coupled NMR Spectroscopy. J. Phys. Chem. Lett. 2025, 16, 5800–5805.

5. Rosencrans, W. M.; Khuntia, H.; Larimi, M. G.; Mahalakshmi, R.; Yu, T.-Y.; Bezrukov, S. M.; Rostovtseva, T. K. Conformational Plasticity of Mitochondrial VDAC2 Controls the Kinetics of Its Interaction with Cytosolic Proteins. Sci. Adv. 2025, 11, eadv4410.

6. Feng, Y.-C.; Agrawal, S.; Yang, C.-H.; Chang, H.-C.; Kuo, L.; Yu, W.-C.; Liu, Y.-T.; Yu, T.-Y. The Structural and Dynamic Insights into the Ala97Ser Amyloidogenic Mutation in Transthyretin. Chem. Asian J. 2024, e202401438.

7. Hsu, C.-H.; Yu, H.-Y.; Lee, H. J.; Wu, P.-H.; Huang, S.-J.; Lee, J. S.; Yu, T.-Y.; Li, Y.-P.; Kang, D.-Y. Fast Water Transport in UTSA-280 via a Knock-off Mechanism. Angew. Chem., Int. Ed. 2023, 62, e202309874.

8. Hu, F.-H.; Chi, L.-T.; Syu, G.-B.; Yu, T.-Y.; Lin, M.-P.; Chen, J.-J.; Yu, W.-Y.; Kang, D.-Y. Mixed-Linker MOF-303 Membranes for Pervaporation. J. Membr. Sci. Lett. 2023, 3, 100053.

9. Zhong, Y.-R.; Yu, T.-Y.; Chu, L.-K. Roles of Functional Lipids in Bacteriorhodopsin Photocycle in Various Delipidated Purple Membranes. Biophys. J. 2022, 121 (10), 1789–1798.

10. Lin, C.-C.; Huang, S.-J.; Wu, P.-H.; Chen, T.-P.; Huang, C.-Y.; Wang, Y.-C.; Chen, P.-T.; Radeva, D.; Petrov, O.; Gelev, V.; Sankar, R.; Chen, C.-C.; Chen, C.-W.; Yu, T.-Y. Direct Investigation of the Reorientational Dynamics of A-site Cations in 2D Organic-Inorganic Hybrid Perovskite by Solid-State NMR. Nat. Commun. 2022, 13, 1513.

11. Liu, C.-H.; Huang, S.-J.; Yu, T.-Y. Cholesterol Modulates the Interaction between HIV-1 Viral Protein R and Membrane. Membranes 2021, 11 (10), 784.

12. Zhong, P. B.; Liu, C.-H.; Chen, Y.-T.; Yu, T.-Y. The Study of HIV-1 Vpr-Membrane and Vpr-hVDAC-1 Interactions by Graphene Field-Effect Transistor Biosensors. ACS Appl. Bio Mater. 2020, 3 (9), 6351–6357.

13. Ibrahim, R.; Yeh, S.-Y.; Lin, K.-P.; Ricardo, F.; Yu, T.-Y.; Chan, C.-C.; Tsai, J.-W.; Liu, Y.-T. Cellular Secretion and Cytotoxicity of Transthyretin Mutant Proteins Underlie Late-Onset Amyloidosis and Neurodegeneration. Cell. Mol. Life Sci. 2020, 77 (7), 1421–1434.

14. Hsieh, F.-J.; Sotoma, S.; Lin, H.-H.; Cheng, C.-Y.; Yu, T.-Y.; Hsieh, C.-L.; Lin, C.-H.; Chang, H.-C. Bioorthogonal Fluorescent Nanodiamonds for Continuous Long-Term Imaging and Tracking of Membrane Proteins. ACS Appl. Mater. Interfaces 2019, 11 (22), 19774–19781.

15. Liu, Y.-T.; Yen, Y.-J.; Ricardo, F.; Chang, Y.; Wu, P.-H.; Huang, S.-J.; Lin, K.-P.; Yu, T.-Y. Biophysical Characterization and Modulation of Transthyretin Ala97Ser. Ann. Clin. Transl. Neurol. 2019, 6 (10), 1961–1970.

16. Kao, Y.-M.; Cheng, C.-H.; Syue, M.-L.; Huang, H.-Y.; Chen, I.-C.; Yu, T.-Y.; Chu, L.-K. Photochemistry of Bacteriorhodopsin with Various Oligomeric Statuses in Controlled Membrane Mimicking Environments: A Spectroscopic Study from Femtoseconds to Milliseconds. J. Phys. Chem. B 2019, 123 (9), 2032–2039.

17. Huang, H. Y.; Syue, M. L.; Chen, I. C.; Yu, T. Y.; Chu, L. K. Influence of Lipid Compositions in the Events of Retinal Schiff Base of Bacteriorhodopsin Embedded in Covalently Circularized Nanodiscs: Thermal Isomerization, Photoisomerization, and Deprotonation. J. Phys. Chem. B 2019, 123 (43), 9123–9133.

18. Eddy, M. T.; Yu, T. Y.; Wagner, G.; Griffin, R. G. Structural Characterization of the Human Membrane Protein VDAC2 in Lipid Bilayers by MAS NMR. J. Biomol. NMR 2019, 73 (8–9), 451–460.

19. Yusuf, Y.; Massiot, J.; Chang, Y.-T.; Wu, P.-H.; Yeh, V.; Kuo, P.-C.; Shiue, J.; Yu, T.-Y. Optimization of the Production of Covalently Circularized Nanodiscs and Their Characterization in Physiological Conditions. Langmuir 2018, 34 (11), 3525–3532.

20. Yeh, V.; Lee, T.-Y.; Chen, C.-W.; Kuo, P.-C.; Shiue, J.; Chu, L.-K.; Yu, T.-Y. Highly Efficient Transfer of 7TM Membrane Protein from Native Membrane to Covalently Circularized Nanodisc. Sci. Rep. 2018, 8, 13501.

21. Shown, I.; Samireddi, S.; Chang, Y.-C.; Putikam, R.; Chang, P.-H.; Sabbah, A.; Fu, F.-Y.; Chen, W.-F.; Wu, C.-I.; Yu, T.-Y.; Chung, P.-W.; Lin, M. C.; Chen, L.-C.; Chen, K.-H. Carbon-Doped SnS2 Nanostructure as a High-Efficiency Solar Fuel Catalyst under Visible Light. Nat. Commun. 2018, 9, 169.

22. Shih, O.; Yeh, Y.-Q.; Liao, K.-F.; Su, C.-J.; Wu, P.-H.; Heenan, R.; Yu, T.-Y.; Jeng, U.-S. Membrane Charging and Swelling upon Calcium Adsorption as Revealed by Phospholipid Nanodiscs. J. Phys. Chem. Lett. 2018, 9 (15), 4287–4293.

23. Yeh, V.; Hsin, Y.; Lee, T.-Y.; Chan, J. C. C.; Yu, T.-Y.; Chu, L.-K. Lipids Influence the Proton Pump Activity of Photosynthetic Protein Embedded in Nanodiscs. RSC Adv. 2016, 6 (91), 88300–88305.

24. Viegas, A.; Viennet, T.; Yu, T.-Y.; Schumann, F.; Bermel, W.; Wagner, G.; Etzkorn, M. UTOPIA NMR: Activating Unexploited Magnetization Using Interleaved Low-Gamma Detection. J. Biomol. NMR 2016, 64 (1), 9–15.

25. Lee, T.-Y.; Yeh, V.; Chuang, J.; Chan, J. C. C.; Chu, L.-K.; Yu, T.-Y. Tuning the Photocycle Kinetics of Bacteriorhodopsin in Lipid Nanodiscs. Biophys. J. 2015, 109 (9), 1899–1906.

26. Eddy, M. T.; Yu, T.-Y. Membranes, Peptides, and Disease: Unraveling the Mechanisms of Viral Proteins with Solid State Nuclear Magnetic Resonance Spectroscopy. Solid State Nucl. Magn. Reson. 2014, 61–62, 1–7.

27. Coote, P.; Leigh, K.; Yu, T.-Y.; Khaneja, N.; Wagner, G.; Arthanari, H. A New Broadband Homonuclear Mixing Pulse for NMR with Low Applied Power. J. Chem. Phys. 2014, 141 (2), 024201.

28. Kim, S. J.; Singh, M.; Wohlrab, A.; Yu, T.-Y.; Patti, G. J.; O'Connor, R. D.; VanNieuwenhze, M.; Schaefer, J. The Isotridecanyl Side Chain of Plusbacin-A3 Is Essential for the Transglycosylase Inhibition of Peptidoglycan Biosynthesis. Biochemistry 2013, 52 (11), 1973–1979.

29. Coote, P.; Arthanari, H.; Yu, T.-Y.; Natarajan, A.; Wagner, G.; Khaneja, N. Pulse Design for Broadband Correlation NMR Spectroscopy by Multi-Rotating Frames. J. Biomol. NMR 2013, 55 (3), 291–302.

30. Yu, T.-Y.; Raschle, T.; Hiller, S.; Wagner, G. Solution NMR Spectroscopic Characterization of Human VDAC-2 in Detergent Micelles and Lipid Bilayer Nanodiscs. Biochim. Biophys. Acta, Biomembr. 2012, 1818 (6), 1562–1569.

31. Li, J. S.; Ruths, J.; Yu, T.-Y.; Arthanari, H.; Wagner, G. Optimal Pulse Design in Quantum Control: A Unified Computational Method. Proc. Natl. Acad. Sci. U. S. A. 2011, 108 (5), 1879–1884.

32. Yu, T.-Y.; Singh, M.; Matsuoka, S.; Patti, G. J.; Potter, G. S.; Schaefer, J. Variability in C3-Plant Cell-Wall Biosynthesis in a High-CO2 Atmosphere by Solid-State NMR Spectroscopy. J. Am. Chem. Soc. 2010, 132 (18), 6335–6341.

33. Stueber, D.; Yu, T.-Y.; Hess, B.; Kremer, K.; O'Connor, R. D.; Schaefer, J. Chain Packing in Polycarbonate Glasses. J. Chem. Phys. 2010, 132 (10), 104901.

34. Gullion, T.; Yu, T.-Y.; Singh, M.; Patti, G. J.; Potter, G. S.; Schaefer, J. Oxygen-17 Appears Only in Protein in Water-Stressed Soybean Leaves Labeled by 17O2. J. Am. Chem. Soc. 2010, 132 (31), 10802–10807.

35. Raschle, T.; Hiller, S.; Yu, T.-Y.; Rice, A. J.; Walz, T.; Wagner, G. Structural and Functional Characterization of the Integral Membrane Protein VDAC-1 in Lipid Bilayer Nanodiscs. J. Am. Chem. Soc. 2009, 131 (49), 17777–17779.

36. Patti, G. J.; Kim, S. J.; Yu, T.-Y.; Dietrich, E.; Tanaka, K. S. E.; Parr, T. R.; Far, A. R.; Schaefer, J. Vancomycin and Oritavancin Have Different Modes of Action in Enterococcus faecium. J. Mol. Biol. 2009, 392 (5), 1178–1191.

37. Yu, T.-Y.; Schaefer, J. REDOR NMR Characterization of DNA Packaging in Bacteriophage T4. J. Mol. Biol. 2008, 382 (4), 1031–1042.

38. Yu, T.-Y.; O'Connor, R. D.; Sivertsen, A. C.; Chiauzzi, C.; Poliks, B.; Fischer, M.; Bacher, A.; Haase, I.; Cushman, M.; Schaefer, J. 15N{31P} REDOR NMR Studies of the Binding of Phosphonate Reaction Intermediate Analogues to Saccharomyces cerevisiae Lumazine Synthase. Biochemistry 2008, 47 (52), 13942–13951.

39. Jiang, P. C.; Yu, T.-Y.; Perng, W. C.; Hwang, L. P. Pore-to-Pore Hopping Model for the Interpretation of the Pulsed Gradient Spin Echo Attenuation of Water Diffusion in Cell Suspension Systems. Biophys. J. 2001, 80 (6), 2493–2504.

40. Hwang, D. W.; Sinha, A. K.; Cheng, C. Y.; Yu, T.-Y.; Hwang, L. P. Water Dynamics on the Surface of MCM-41 via 2H Double Quantum Filtered NMR and Relaxation Measurements. J. Phys. Chem. B 2001, 105 (24), 5713–5721.

41. Yu, T.-Y.; Cheng, C. Y.; Hwang, D. W.; Huang, H. W.; Hwang, L. P. Water-Filled MCM-41 Characterized by Double-Quantum-Filtered 2H NMR Spectral Analysis. Appl. Magn. Reson. 2000, 18 (3), 435–453.

42. Hedin, N.; Yu, T.-Y.; Furo, I. Growth of C12E8 Micelles with Increasing Temperature. A Convection-Compensated PGSE NMR Study. Langmuir 2000, 16 (19), 7548–7550.

背景圖 畫家: 余享諦