Genetic tests for low- and middle-income countries: Practical course

1st Day (8 hrs – break in between)

Group A (5 persons)

Group B (5 persons)

2nd Day (8 hrs – break in between)

Group A (5 persons)

Group B (5 persons)

PROGRAM

Working Instructions (IL)/

- IL 7.5.1_01 Extraction of DNA from blood Mini Kit

- IL 7.5.1_04 Gel electrophoresis

- IL 7.5.1_05 PCR amplification of DNA

- Gel PCR

- Gel restriction enzyme digestion

Forms/

- Mod 7.5.1-02 Informed consent

- Mod 7.5.1-04 PCR amplification worksheet

- Mod 7.5.1-06 Report

- Mod 7.5.1-09 Worksheet restriction enzyme digestion

- Mod 7.5.1-13 Clinical record of patient

PO/

- PO 7.5.1-01 Planning and provision of molecular biology service

- PO 7.5.1-02 Instructions for sending samples

Protocols/

PROTOCOL: HYPERTENSION-INDUCED NEPHROPATHY (Associated gene ACE)

PROTOCOL: LACTOSE INTOLERANCE (Associated gene MCM6)

PROTOCOL: SPINAL MUSCLE ATROPHY (Associated gene SMN1)

Reports/

References:

1) Precone V., Dundar M., Beccari T, Turanli ET., Cecchin S., Marceddu G., Manara E., Bertelli M.; Quality assurance of geneticlaboratories and the EBTNA practicecertification, a simplestandardizationassurance system for a laboratory network; The EuroBiotech Journal 2017, 4, 215–258;

2) Maltese PE, Poplavskaia E, Malyutkina I, Sirocco F, Bonizzato A, Capodicasa N, Nicoulina SY, Salmina A, Aksutina N, Dundar M, Beccari T, Cecchin S, Bertelli M. Genetic tests for low- and middle-income countries: a literature review. GenetMol Res. 2017 Feb 8; 16(1).