• Research staff at the National Veterinary Assay Laboratory- April 2006 to April 2012.

• Associate professor at the Laboratory of Food Microbiology and Food safety, School of Veterinary Medicine, Rakuno Gakuen University- May 2012 to

Recent publications

1. Usui M, Tateno S, Onozaki M, Misawa N, Suzuki Y, Tamura Y, et al. Rapid and simultaneous detection of fluoroquinolone- and macrolide-resistant Campylobacter jejuni/coli in retail chicken meat using CAMpylobacter Express Resistance Array (CAMERA). Food Control. 2021;123:107815.

2. Usui M, Nozawa Y, Fukuda A, Sato T, Yamada M, Makita K, et al. Decreased colistin resistance and mcr-1 prevalence in pig-derived Escherichia coli in Japan after banning colistin as a feed additive. Journal of global antimicrobial resistance. 2021;24:383-6.

3. Tsunoda R, Usui M, Tagaki C, Fukuda A, Boonla C, Anomasiri W, et al. Genetic characterization of coliform bacterial isolates from environmental water in Thailand. Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy. 2021;27(5):722-8.

4. Sabala RF, Usui M, Tamura Y, Abd-Elghany SM, Sallam KI, Elgazzar MM. Prevalence of colistin-resistant Escherichia coli harbouring mcr-1 in raw beef and ready-to-eat beef products in Egypt. Food Control. 2021;119:107436.

5. Fukuda A, Nakamura H, Umeda K, Yamamoto K, Hirai Y, Usui M, et al. Seven-year surveillance of the prevalence of antimicrobial-resistant Escherichia coli isolates, with a focus on ST131 clones, among healthy people in Osaka, Japan. International journal of antimicrobial agents. 2021;57(3):106298.

6. Yossapol M, Suzuki K, Odoi JO, Sugiyama M, Usui M, Asai T. Persistence of extended-spectrum β-lactamase plasmids among Enterobacteriaceae in commercial broiler farms. Microbiol Immunol. 2020;64(10):712-8.

7. Yoshizawa N, Usui M, Fukuda A, Asai T, Higuchi H, Okamoto E, et al. Manure Compost Is a Potential Source of Tetracycline-Resistant Escherichia coli and Tetracycline Resistance Genes in Japanese Farms. Antibiotics (Basel, Switzerland). 2020;9(2).

8. Usui M, Maruko A, Harada M, Kawabata F, Sudo T, Noto S, et al. Prevalence and characterization of Clostridioides difficile isolates from retail food products (vegetables and meats) in Japan. Anaerobe. 2020;61:102132.

9. Usui M. One Health approach to Clostridioides difficile in Japan. Journal of Infection and Chemotherapy. 2020;26(7):643-50.

10. Thongratsakul S, Usui M, Higuchi H, Takahashi T, Sato T, Poolkhet C, et al. Prevalence and characterization of Staphylococcus aureus isolated in raw milk from cows in Hokkaido, Japan. Tropical animal health and production. 2020;52(4):1631-7.

11. Munby M, Fujiki J, Aoki K, Kawaguchi C, Nakamura K, Nakamura T, et al. Whole-Genome Sequence of Fluoroquinolone-Resistant Escherichia coli HUE1, Isolated in Hokkaido, Japan. Microbiol Resour Announc. 2020;9(46).

12. Makita K, Fujimoto Y, Sugahara N, Miyama T, Usui M, Asai T, et al. Quantitative Release Assessment of <i>mcr</i>-mediated Colistin-resistant <i>Escherichia Coli</i> from Japanese Pigs. Food Safety. 2020;8(2):13-33.

13. Fukuda A, Usui M, Tamura Y. Roles of Flies in Bacterial Transmission, Maintenance, and Contamination as Vectors and Reservoirs. 2020.

14. Fukuda A, Usui, M., Tagaki, C., Sukpanyatham, N., Tamura, Y. Escherichia coli carrying cephalosporin (bla) and colistin (mcr) resistance genes isolated from broilers and pigs in Thailand. Trop Med Public Health. 2020;51:251-61.

15. Fujiki J, Furusawa T, Munby M, Kawaguchi C, Matsuda Y, Shiokura Y, et al. Susceptibility of Pseudomonas aeruginosa veterinary isolates to Pbunavirus PB1-like phages. Microbiology and Immunology. 2020;64(11):778-82.

16. Asai T, Usui M, Sugiyama M, Izumi K, Ikeda T, Andoh M. Antimicrobial susceptibility of Escherichia coli isolates obtained from wild mammals between 2013 and 2017 in Japan. The Journal of veterinary medical science / the Japanese Society of Veterinary Science. 2020;82(3):345-9.

17. Usui M, Yokoo H, Tamura Y, Nakajima C, Suzuki Y, Ghigo JM, et al. Zinc Acetate Potentiates the Action of Tosufloxacin against Escherichia coli Biofilm Persisters. Antimicrobial agents and chemotherapy. 2019;63(6).

18. Usui M, Ozeki K, Komatsu T, Fukuda A, Tamura Y. Prevalence of Extended-Spectrum β-Lactamase–Producing Bacteria on Fresh Vegetables in Japan. Journal of Food Protection. 2019;82(10):1663-6.

19. Usui M, Kajino A, Kon M, Fukuda A, Sato T, Shirakawa T, et al. Prevalence of 16S rRNA methylases in Gram-negative bacteria derived from companion animals and livestock in Japan. The Journal of veterinary medical science / the Japanese Society of Veterinary Science. 2019;81(6):874-8.

20. Okubo T, Hasegawa T, Fukuda A, Thapa J, Usui M, Tamura Y, et al. Screening of hospital-manhole sewage using MacConkey agar with cefotaxime reveals extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli. International journal of antimicrobial agents. 2019;54(6):831-3.

21. Okubo T, Ae R, Noda J, Iizuka Y, Usui M, Tamura Y. Detection of the sul2–strA–strB gene cluster in an ice core from Dome Fuji Station, East Antarctica. Journal of global antimicrobial resistance. 2019;17:72-8.

22. Kudo H, Usui M, Nagafuji W, Oka K, Takahashi M, Yamaguchi H, et al. Inhibition effect of flavophospholipol on conjugative transfer of the extended-spectrum beta-lactamase and vanA genes. The Journal of antibiotics. 2019;72(2):79-85.

23. Fukuda A, Usui M, Okamura M, Dong-Liang H, Tamura Y. Role of Flies in the Maintenance of Antimicrobial Resistance in Farm Environments. Microbial drug resistance. 2019;25(1):127-32.

24. Fukuda A, Usui M, Masui C, Tamura Y. Quantitative Analysis of Houseflies-mediated Food Contamination with Bacteria. Food Safety. 2019;7(1):11-4.

25. Azam AH, Kadoi K, Miyanaga K, Usui M, Tamura Y, Cui L, et al. Analysis host-recognition mechanism of staphylococcal kayvirus ɸSA039 reveals a novel strategy that protects Staphylococcus aureus against infection by Staphylococcus pseudintermedius Siphoviridae phages. Applied microbiology and biotechnology. 2019.

26. Usui M HM, Kawabata F, Sato T, Higuchi H, Tamura Y. Prevalence of Clostridium diffcile in Japanese cows and calves. J Jpn Vet Med Assoc. 2018;71:261-5.

B.S., University of Hartford, Hartford, CT, USA, 1970-1973

M.S. University of Connecticut, Storrs, CT, 1974-1976

Ph.D. Department of Immunology and Microbiology, School of Medicine, Wayne State University, Detroit, MI, 1976-1979

Postdoc., Department of Immunology and Microbiology, School of Medicine, Wayne State University, Detroit, MI, 1979-1980

Staff Fellow, Department of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, 1981-1984; Animal Parasitic Diseases Laboratory, USDA-ARS, Beltsville, MD. 1984-2013; Animal Biosciences and Biotechnology Laboratory, USDA-ARS, Beltsville, MD. 2014-present; 2014

Promoted to the Supergrade Rank at USDA-ARS; 2015

Inducted into USDA-ARS Hall of Fame

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Lillehoj has been a pioneer in study of poultry immunopathology of pathogens, esp. Eimeria and Clostridium perfringens, covering genomics in dissecting the molecular basis of immune response and disease protection. Her work has led to new insights in protective immune mechanisms that facilitated the development of alternatives to antibiotic immunotherapeutics.

Awards:

1. Federal Laboratory Consortium (FLC) Technology Transfer Award (1999)

2. Pfizer Fundamental Science Award, 2007; Phibro Animal Health Excellence in Research Award (2011)

3. ARS Outstanding Senior Scientist of the Year, 2014

4. Distinguished Veterinary Immunologist, American Association of Veterinary Immunologist, 2014

5. Presidential Rank Award, USDA, 2015

6. American Medal for Career Achievement, USA, 2016. Seventeen U.S. Patents, and 65 commercial licensing of immune tools, 1986-2021.

Education

Department of Animal Husbandry, National Taiwan University. Master degree (1979-1981)

Department of Dairy Science, University of Wisconsin-Madison. Ph. D degree (USA) (1989-1993)

Major Research

Nutrition for dairy cow and dairy goat and the farm management

Exploration of the alternative feed resource like local forage, agricultural by-products, and insect protein

Mitigation of enteric methane emission from diet manipulation

Education

1988.9-1994.6 Bachelor National Taiwan University School of Veterinary Medicine

1997.9-2003.1 Doctoral National Taiwan University Graduate Institute Veterinary

Research Interest

Mycoplasma

Veterinary Microbiology

Immunology

Molecular Biology

Risk Assessment

Meat Safety

Antimicrobial Resistance

Experience

Agricultural Technology Research Institute, Animal Technology Laboratories Director

Animal Technology Institute Taiwan, Animal Medicine, Head of Division of Animal Medicine and Associate Research Fellow 2012.1-2013.12

Animal Technology Institute Taiwan, Animal Medicine, Associate Research Fellow 2004.10-2011.12

Animal Technology Institute Taiwan, Animal Medicine, Postdoctor 2003.3-2004.10

Pig Research Institute Taiwan Pathobiology, Research Assistant 1996.6-1997.9

1969~1981

National Research institute for Animal Health

Researcher and Head of Dept. for Animal Vaccine Research and Production

1981~2009

college of Veterinary Meclizine National Chung-Hsing University

Professor, Head of Department, Dean of the college

1996~1997

Department of Animal Industry Council of Agriculture Executive yuan

Head of the Department

Working Experience

2014.1. – 2018.6 Director & Researcher, Animal Drugs Inspection Branch, Animal Health Research Institute, Executive Yuan

2009.3. -2014.1 Director & Researcher, Epidemiology Research Division, Animal Health Research Institute, Council of Agriculture, Executive Yuan

2008.7- 2009.3. Deputy Director General & Researcher, Animal Health Research Institute , Council of Agriculture, Executive Yuan

2004 -2008.7 Director & Researcher, Epidemiology Research Division, Animal Health Research Institute, Council of Agriculture, Executive Yuan

Reference

1. Shu-Hwae LEE, Yi-Ping LU, Wen-Ling SHIH, Ching-Dong CHANG, Yang-Chang TU, and I-Hsiang LAI^,* Development of an immunoassay using recombinant outer membrane protein A and flagellin for diagnosis of goats with melioidosis. J Vet Med Sci. 2020 Mar; 82(3): 325–332.

2. Yang-Chang Tu, Kuang-Yu Chen, Chung-Kung Chen, Ming-Chu Cheng, Shu-Hwae Lee, Ivan-Chen Cheng. Novel application of Influenza A virus-inoculated chorioallantoic membrane to characterize a NP-specific monoclonal antibody for immunohistochemistry assaying. J Vet Sci. 2019 Jan; 20(1): 51–57.

3. Shu-Chia Hu, Chao-Lung Hsu, Ming-Shiuh Lee, Yang-Chang Tu, Jen-Chieh Chang, Chieh-Hao Wu, Shu-Hwae Lee, Lu-Jen Ting, Kwok-Rong Tsai, Ming-Chu Cheng, Wen-Jane Tu, and Wei-Cheng Hs。 EID (2018) Lyssavirus in Japanese Pipistrelle, Taiwan

4. Ai-Ping Hsu,Chun-Hsien Tseng, Jacques Barrat, Shu-Hwae Lee, Yu-Hua Shih,Marine Wasniewski,Philippe Mähl,Chia-Chia Chang,Chun-Ta Lin,Re-Shang Chen, Wen-Jane Tu Florence Cliquet ,Hsiang-Jung Tsai (2017) Safety, efficacy and immunogenicity evaluation of the SAG2 oral rabies vaccine in Formosan ferret badgers. ,https://doi.org/10.1371/journal.pone.0184831

5. Shu-Chia Hu, Chao-Lung Hsu, Ming-Shiuh Lee, Yang-Chang Tu, Jen-Chieh Chang, Chieh-Hao Wu, Shu-Hwae Lee, Lu-Jen Ting, Kwok-Rong Tsai, Ming-Chu Cheng, Wen-Jane Tu, and Wei-Cheng Hsu(2018) Lyssavirus in Japanese Pipistrelle, Taiwan. Emerg Infect Dis. 2018 Apr; 24(4): 782–785.

6. K.J. Tsai, W.C. Hsu, W.C. Chuang, J.C. Chang, Y.C. Tu, H.J. Tsai,c, H.F. Liu, F.I.Wang, S.H. Lee (2016, Jan). Emergence of a sylvatic enzootic formosan ferret badger-associated rabies in Taiwan and the geographical separation of two phylogenetic groups of rabies viruses. Veterinary Microbiology, 2016, 182, 28-34.

7. Jen-Chieh Chang, Kuo-Jung Tsai, Wei-Cheng Hsu1, Yang-Chang Tu1,Wei- 3 Chieh Chuang1, Chia-Yi Chang1, Shih-Wei Chang2, Te-En Lin2, Kuo-Yun Fang2, Yung-Fu 4 Chang4, Hsiang-Jung Tsai1, and Shu-Hwae Lee (2015, Oct). Rabies virus infection in ferret badgers in Taiwan: a retrospective study. Journal of Wildlife Diseases, 2015, 51(4), 923–928.

8. Lih-Chiann Wang, Dean Huang, Ming-Chu Cheng, Shu-Hwae Lee, Ching-Ho Wang (2015). H5 Avian Inflenza Virus Pathotyping Using Oligonucleotide Microarry. Journal of Virological Methods, 2015,220,39-42.

9. Lu-Jen Ting, Ming-Shiuh Lee, Shu-Hwae Lee, Hsiang-Jung Tsai, Fan Lee (2014,May). Relationships of bovine ephemeral fever epizootics to population immunity and virus variation. Veterinary Microbiology.

10. Chun Wang, Victor Fei Pang, Fan Lee, Tien-Shine Huang, Shu-Hwae Lee, Yu-Ju Lin, Yeou-Liang Lin, Shiow-Suey Lai, Chian-Ren Jeng. (2013). Prevalence and genetic variation of porcine circovirus type 2 in Taiwan from 2001 to 2011.Research in Veterinary Science, 94 (789–795).

11. Chen, Y.P. Lee, S.H., Chou, C.H., Tsai, H.J. (2012, --). Detection of florfenicol resistance genes in Riemerella anatipestifer isolated from ducks and geese.. Vet.Microbiol., 154: 325-331. (SCI).

12. Yen-Ping Chen, Ming-Yang Tsao, Shu-Hwae Lee, Chung-Hsi Chou, Hsiang-Jung Tsai. (2012, --). Prevalence and molecular characterization of chloramphenicol resistance in Riemerella anatipestifer isolated from ducks and geese in Taiwan..Avian pathology., 39:333-338. (SCI).

13. Kosuke SODA, Ming-Chu CHENG, Hiromi YOSHID, Mayumi ENDO, Shu- Hwae LEE ,Masatoshi OKAMATSU, Yoshihiro SAKODA, Ching-Ho WANG,Hiroshi KIDA. (2011, --). A low pathogenic H5N2 influenza virus isolated in Taiwan acquired high pathogenicity by consecutive passages in chickens. . The Journal of Veterinary Medical Science., 73:767-772.. (SCI).

14. Cheng MC, Soda K, Lee MS, Lee SH, Sakoda Y, Kida H, Wang CH. (2010, --).Isolation and characterization of potentially pathogenic H5N2 influenza virus from a chicken in Taiwan in 2008.. Avian Dis, 54: 885-893. (SCI).

15. Lee MS, Tsai KJ, Chen LH, Chen CY, Liu YP, Chang CC, Lee SH, Hsu WL (2010, --). The identification of frequent variations in the fusion protein of canine distemper virus.. The Vet J, 183: 184–190. (SCI).

16. Karuppannan AK, Jong MH, Lee SH, Zhu Y, Selvaraj M, Lau J, Jia Q, Kwang J.(2009, --). Attenuation of porcine circovirus 2 in SPF piglets by abrogation of ORF3 function. . Virology, 383: 338–347,. (SCI).

17. Lee SH, Jong MH, Huang TS, Lin YL, Wong ML, Liu CI, Chang TJ (2009, --).Pathology and Viral Distributions of the Porcinophilic Foot-And-Mouth Disease Virus Strain (O/Taiwan/97) in Experimentally Infected Pigs. . Transbound Emerg Dis, 56: 189–201. (SCI).

18. Lee SH, Lin HC, Chiang HY, Chang KH, Cheng MC, Wang CH, Chang TJ.(2009, --). Case Report: The First Case of Anthrax in a Horse in Taiwan.. Taiwan Vet J, 35: 241-247..

19. Wang LC, Pan CH, Severinghaus LL, Liu LY, Chen CT, Pu CE, Huang D, Lir JT,Chin SC, Cheng MC, Lee SH, Wang CH. (2008, --). Simultaneous detection and differentiation of Newcastle disease and avian influenza viruses using oligonucleotide microarrays.. Vet Microbiol, 127: 217-226. (SCI).

20. Chan KW, Lin JW, Lee SH, Liao CJ, Tsai MC, Hsu WL, Wong ML, Shih HC.(2007, --). Identification and phylogenetic analysis of orf virus from goats in Taiwan. . Virus Genes , 35: 705-712. (SCI).

Dr. Shimoji is currently working as a manager of the Division of Infectious Animal Disease Research, National Institute of Animal Health (NIAH), National Agriculture and Food Research Organization (NARO), Japan. He is also a visiting professor at Research Institute for Biomedical Sciences, Tokyo University of Science, Chiba, Japan.

He is a bacteriologist with broad interests in bacterial infections of animal and humans. He has long been working on erysipelas infection, which is mainly caused by the gram-positive bacterium Erysipelothrix rhusiopathiae. His other main interests include vaccine development for animal diseases and the discovery of new therapeutics for bacterial infections.

Selected Publications:

1. Shimoji Y, Shiraiwa K, Tominaga H, Nishikawa S, Eguchi M, Hikono H, Ogawa Y. Development of a multiplex PCR-based assay for rapid serotyping of Erysipelothrix species. J Clin Microbiol. 58(6):e00315-20. 2020.

2. Shimoji Y, Ogawa Y, Tsukio M, Shiraiwa K, Nishikawa S, Eguchi M. Genome-wide identification of virulence genes in Erysipelothrix rhusiopathiae: use of a mutant deficient in a tagF homolog as a safe oral vaccine against swine erysipelas. Infect Immun. 87(12):e00673-19. 2019.

3. Shimoji Y, Tsukio M, Ogawa Y, Shiraiwa K, Nishikawa S, Eguchi M. A putative transcription regulator involved in the virulence attenuation of an acriflavine-resistant vaccine strain of Erysipelothrix rhusiopathiae, the causative agent of swine erysipelas. Vet Microbiol. 239:108488. 2019.

4. Ogawa Y, Shiraiwa K, Nishikawa S, Eguchi M, Shimoji Y. Identification of the chromosomal region essential for serovar-specific antigen and virulence of serovar 1 and 2 strains of Erysipelothrix rhusiopathiae. Infect Immun. pii: IAI.00324-18. 86(9):e00324-18. 2018.

5. Nishikawa S, Ogawa Y, Eguchi M, Rambukkana A, Shimoji Y. Draft genome sequences of Lawsonia intracellularis swine strains causing proliferative enteropathy in Japan. Microbiol Resour Announc. 7(9):e01021-18. 2018.

6. Shiraiwa K, Ogawa Y, Eguchi M, Hikono H, Kusumoto M, Shimoji Y. Development of an SNP-based PCR assay for rapid differentiation of a Japanese live vaccine strain from field isolates of Erysipelothrix rhusiopathiae. J Microbiol Methods. 117:11-13. 2015.

7. Shi F, Ogawa Y, Sano A, Harada T, Hirota J, Eguchi M, Oishi E, Shimoji Y. Characterization and identification of a novel candidate vaccine protein through systematic analysis of extracellular proteins of Erysipelothrix rhusiopathiae. Infect Immun. 81(12):4333-4340. 2013.

8. Ogawa Y, Minagawa Y, Shi F, Eguchi M, Muneta Y, Shimoji Y. Immunostimulatory effects of recombinant Erysipelothrix rhusiopathiae expressing porcine interleukin-18 in mice and pigs. Clin Vaccine Immunol. 19(9):1393-1398. 2012.

9. Ogawa Y, Ooka T, Shi F, Ogura Y, Nakayama K, Hayashi T, Shimoji Y. The genome of Erysipelothrix rhusiopathiae, the causative agent of swine erysipelas, reveals new insights into the evolution of Firmicutes and the organism's intracellular adaptations. J Bacteriol. 193(12):2959-2971. 2011.

10. Ogawa Y, Oishi E, Muneta Y, Sano A, Hikono H, Shibahara T, Yagi Y, Shimoji Y. Oral vaccination against mycoplasmal pneumonia of swine using a live Erysipelothrix rhusiopathiae vaccine strain as a vector. Vaccine. 27(33):4543-4550. 2009.

11. Shimoji Y, Ogawa Y, Osaki M, Kabeya H, Maruyama S, Mikami T, Sekizaki T. Adhesive surface proteins of Erysipelothrix rhusiopathiae bind to polystyrene, fibronectin, and type I and IV collagens. J Bacteriol. 185(9):2739-2748. 2003.

12. Shimoji Y, Oishi E, Muneta Y, Nosaka H, Mori Y. Vaccine efficacy of the attenuated Erysipelothrix rhusiopathiae YS-19 expressing a recombinant protein of Mycoplasma hyopneumoniae P97 adhesin against mycoplasmal pneumonia of swine. Vaccine. 21(5-6):532-537. 2003.

13. Shimoji Y, Oishi E, Kitajima T, Muneta Y, Shimizu S, Mori Y. Erysipelothrix rhusiopathiae YS-1 as a live vaccine vehicle for heterologous protein expression and intranasal immunization of pigs. Infect Immun. 70(1):226-232. 2002.

14. Shimoji Y. Review: Pathogenicity of Erysipelothrix rhusiopathiae: virulence factors and protective immunity. Microbes Infect. 2(8):965-72. 2000.

15. Shimoji Y, Ng V, Matsumura K, Fischetti VA, Rambukkana A. A 21-kDa surface protein of Mycobacterium leprae binds peripheral nerve laminin-2 and mediates Schwann cell invasion. Proc Natl Acad Sci USA. 96(17):9857-9862. 1999.

16. Shimoji Y, Mori Y, Fischetti VA. Immunological characterization of a protective antigen of Erysipelothrix rhusiopathiae: identification of the region responsible for protective immunity. Infect Immun. 67(4):1646-1651. 1999.

17. Shimoji Y, Mori Y, Hyakutake K, Sekizaki T, Yokomizo Y. Use of an enrichment broth cultivation-PCR combination assay for rapid diagnosis of swine erysipelas. J Clin Microbiol. 36(1):86-9. 1998.

18. Shimoji Y, Mori Y, Sekizaki T, Shibahara T, Yokomizo Y. Construction and vaccine potential of acapsular mutants of Erysipelothrix rhusiopathiae: use of excision of Tn916 to inactivate a target gene. Infect Immun. 66(7):3250-3254. 1998.

19. Shimoji Y, Yokomizo Y, Mori Y. Intracellular survival and replication of Erysipelothrix rhusiopathiae within murine macrophages: failure of induction of the oxidative burst of macrophages. Infect Immun. 64(5):1789-1793. 1996.

20. Shimoji Y, Yokomizo Y, Sekizaki T, Mori Y, Kubo M. Presence of a capsule in Erysipelothrix rhusiopathiae and its relationship to virulence for mice. Infect Immun. 62(7):2806-2810. 1994.

Head of the Division of Pharmacology and Toxicology

Faculty of Veterinary Medicine – Bogor Agricultural University (IPB University)

Director/ Institutional Official

Pusat Studi Satwa Primata (Primate Research Center) – IPB University

Research Experience:

Non-human primate (NHP) model of neurodegenerative and cognition disease of Alzheimer’s Type (2011-now)

Surgical and chemically induced of rat’s model for aneurism studies (2016-2018)

Colistin-resistance studies in poultry chain production (2017-2019)

Edible bird nest activity as an antiaging for mesenchymal stem cell (2018-now)

Elisa kit of amyloid beta 42 for Alzheimer diagnosis (2019-now)

Development of NHP model for Vaccine Human Papilloma Virus (2019-now)

Safety assessment of anti-cancer drugs of HER-2 monoclonal antibodies of PT Biofarma, Indonesia (2019-now)

Non-human primate for Human Papilloma Virus studies – therapeutic and preventive vaccine (2019-now)

Histopathology and molecular markers of Alzheimer’s type disease in senile cynomolgus monkeys (2019-now)

Publications:

access: https://scholar.google.com/citations?user=ZDj4AcAAAAAJ&hl=en

ACADEMIC BACKGROUND

DVM (Magna Cum Laude), 2007, University of the Philippines Los Baños

DVSc (PhD, Veterinary Molecular Epidemiology), 2014, Yamaguchi University, Japan

SELECTED HONORS, AWARDS AND GRANTS

2016 Awardee, Young Poultry Veterinarian of the Year Award, World Veterinary Poultry Association (WVPA)

2016 Recipient, UP Scientific Productivity Award (UP Scientist I), University of the Philippines - System

2015 Awardee, Outstanding Young Scientist of the Philippines, National Academy of Science and Technology-Philippines

2014 Recipient, Postdoctoral Training and Research Fellowship, Poultry Products Quality Control, Japan

2012-2019 Recipient, International Publication Award, University of the Philippines System

2012 Best Paper Presentation, Annual Veterinary Science Seminar, The United Graduate School of Veterinary Sciences, Yamaguchi University, Japan

2009-2014 Recipient, Masako and Hiromitsu Katoh Work/Straight-PhD Scholarship Grant

2007 Board Topnotcher (Top 1, 85.25%), 2007 Philippine Veterinary Medicine Licensure Examination

2007 Awardee, 2007 Ten Outstanding Students of the Philippines

RESEARCH OUTPUTS

Have authored and published more than 30 research articles on disease diagnosis, molecular characterization of livestock and poultry pathogens, vaccine development and other research works involving emerging diagnostic laboratory techniques in the last 10 years; and have delivered more than a hundred technical presentations in international conferences, scientific seminars and training workshops spanning 10 countries.

PROJECT INVOLVEMENTS

Proponent/Project Leader. Low-cost Portable Molecular Diagnostic Platform for Rapid Detection of Poultry Infectious Pathogens (LMDP) Newton Fund Swine and Poultry Research Initiative. 2018-2021

Proponent/Project Leader. Isolation and Molecular Characterization of Salmonella spp. in Commercial Chicken Eggs from Public Markets in Metro Manila, Philippines. University of the Philippines Enhanced Creative Work and Research Grant (ECWRG). 2017-2018.

Co-Proponent/Project Co-leader. Surveillance of Porcine Epidemic Diarrhea (PED) and Transmissible Gastroenteritis (TGE) in commercial pig farms in Luzon, Philippines. PL-480 Title 1 Program (Profiling of Economically Important Diseases of Swine and Cattle in The Philippines for Enhanced Disease Management, Surveillance and Control). 2016-2018

Proponent/Project Leader Molecular Characterization of Newcastle Disease Virus in the Philippines. DOST Grants for Outstanding Achievements in Science and Technology. 2016-2017.

Proponent/Project Leader Molecular Characterization of Economically Important Poultry Pathogens in the Philippines. Postdoctoral Training and Research Fellow, Poultry Products Quality Control, Japan. 2014-present

Proponent/Project Leader Molecular Epidemiology of Newcastle Disease Virus in Japan, Postdoctoral Training and Research Fellow, Poultry Products Quality Control (PPQC Co.) Straight PhD Research Program. 2014-2019.

SELECTED PUBLICATIONS

1. FP Del Valle, SI Camba, DV Umali, K. Sasai, K. Shirota, H. Katoh and T. Tajima. 2020. Molecular and pathologic characterization of avian adenovirus isolated from the oviducts of laying hens in eastern Japan. Poultry Sciences 99 (5): 2459-2568.

2. SI Camba, FP Del Valle, DV Umali, T Soma, K. Shirota, H. Katoh and K. Sasai. 2020. The expanded role of roof-rats (Rattus rattus) in Salmonella spp. Contamination of a commercial layer farm in east Japan. Avian Diseases 64(1): 46-52.

3. DV Umali, K Shirota, K Sasai and H Katoh. 2018. Characterization of Ornithobacterium rhinotracheale from commercial layer chickens in Eastern Japan. Poultry Science 97(1): 24-29.

4. DV Umali, AM Lopez, MP Torres, MC Umandal and H. Katoh. 2017. Molecular characterization and phylogenetic analysis of Newcastle disease virus isolated from clinical cases of Newcastle disease in commercial layer farms in the Philippines. The Philippine Journal of Veterinary Medicine 54(1): 17-26.

5. DV Umali, H Ito, T Suzuki, K Shirota, T Ito and H. Katoh. 2015. Atypical velogenic Newcastle disease in a commercial layer flock In Japan. Poultry Science 94(5): 890-897.

6. DV Umali, A. Ito, FP del Valle, T Suzuki, K Shirota, H. Katoh. 2014. Evaluation of Antibody Response, Interferon-Gamma Expression and Protection levels of Chickens Vaccinated with Booster Dose of Recombinant Oil-Adjuvanated Leucocytoozoon caulleryi (Mathis and Leger, 1909) Subunit Vaccine. Avian Diseases 58(4): 609-615.

7. DV Umali, H Ito, T Suzuki, K Shirota, H. Katoh and T Ito. 2014. Characterization of Complete Genome Sequence of Genotype VI and VII Velogenic Newcastle Disease Virus from Japan. Virus Genes 49: 89-99. DOI: 10.1007/s11262-014-1075-7.

8. DV Umali, H Ito, H. Katoh and T Ito. 2014. Surveillance of Avian Paramyxovirus in Migratory Waterfowls in the San-in Region of Western Japan from 2006-2012. Journal of Veterinary Medical Science 76(3): 423-30

9. DV Umali, H Ito, T Suzuki, K Shirota, H. Katoh and T Ito. 2013. Molecular Epidemiology of Newcastle Disease Virus Isolates from Vaccinated Commercial Poultry Farms in Non-Epidemic Areas of Japan. Virology Journal 10: 330

10. K Shirota, DV Umali, T Suzuki, and H Katoh. 2012 Epizootiologic Role of Feeds in the Epidemiology of Salmonella Senftenberg Contamination in Commercial Layer Farms in Eastern Japan. Avian Diseases 56(3): 516-520.

11. DV Umali, RRSP Lapuz, T Suzuki, K Shirota, and H Katoh. 2012. Transmission and Shedding Patterns of Salmonella in Naturally Infected Captive Wild Roof Rats (Rattus rattus) from a Salmonella-Contaminated Layer Farm. Avian Diseases 56(2): 288-294

Publications (Journal articles)

1. Hsu AP, Tseng CH, Shih YH, Tsai KJ, Lu YT, Chou CH, Tu WJ and Tsai HJ. Pathogenicity of Taiwan ferret badger rabies virus in rodents. Taiwan Vet J. 2018;44:133-139.

2. Hsu AP, Tseng CH, Lu YT, Shih YH, Chou CH, Chen RS, Tsai KJ, Tu WJ, Cliquet F, Tsai HJ. Development of a quantitative real-time RT-PCR assay for detecting Taiwan ferret badger rabies virus in ear tissue of ferret badgers and mice. J Vet Med Sci. 2018;6:1012-1019.

3. Hsu AP, Tseng CH, Barrat J, Lee SH, Shih YH, Wasniewski M, Mähl P, Chang CC, Lin CT, Chen RS, Tu WJ, Cliquet F, Tsai HJ. Safety, efficacy and immunogenicity evaluation of the SAG2 oral rabies vaccine in Formosan ferret badgers. PLoS One. 2017;12:e0184831.

4. Hsu AP, Lin YJ, Lee YL, Tseng CH, Huang TS. Development of Bovine Ephemeral Fever G Protein Subunit Vaccines. Exp.Rep AHRI. 2013;48:29-38

5. Lin KH, Lin CF, Chiou SS, Hsu AP, Lee MS, Chang CC, Chang TJ, Shien JH, Hsu WL. Application of purified recombinant antigenic spike fragments to the diagnosis of avian infectious bronchitis virus infection. Appl Microbiol Biotechnol. 2012 Jul;95(1):233-42.

6. Lin KH, Hsu AP, Shien JH, Chang TJ, Liao JW, Chen JR, Lin CF, Hsu WL. Application of purified recombinant antigenic spike fragments to the diagnosis of avian infectious bronchitis virus infection. Vaccine. 2012 Jul 13;30(33):5019-29.

Publications (Conferences/Seminars)

1. Hsu AP, Chang CC, Shih YH, Lin CT, Tsai JH, Yeh SR, et al. The antibody responses of Formosan ferret badgers after receiving rabies inactivated vaccines. Rabies serology meeting. Budapest, Hungry (June 2017).

2. Hsu AP, Tseng CH, Shih YH, Chou CH, Florence Cliquet, Tsai HJ, et al. Replication properties of Taiwan ferret badger rabies virus in mice. RITA (Rabies in the Americas). Fort Collins, the US (Oct 2015).

3. Hsu AP, Shih YH, Liu HF, Lin YR, Lee SH, Tseng CH, et al. Sequence Analysis of Ferret Badger Rabies Virus from Taiwan Outbreak in 2013. IUMS. Montreal, Canada. (Aug 2014).

4. Hsu AP, Lu YT, Shih YH, Chen H, Tu WJ, Tseng CH, Tsai HJ. Development of Newcastle Disease Live Vaccine of Genotype VII and the Virulence Determination. Conference of Chinese Society of Veterinary Sciences. Taipei (Dec 2017)

5. Hsu AP, Shih YH, Lu YT, Chen H, Chen RS, Tu WJ, et al. Rabies Virus Detection in Diversified Organs of Mice by Real-time RT-PCR. Conference of Chinese Society of Veterinary Sciences. Taichung (Nov 2016)

6. Hsu AP, Shih YH, Lu YT, Hu HH, Chen RS, Tu WJ, et al. The Development of Newcastle Disease Vector Vaccines. Conference of Chinese Society of Veterinary Sciences. Taichung (Nov 2016)

Dr. Yang obtained his Ph.D. from the Université de la Méditerranée, France. He was a visiting scholar at Umass School of Medicine, and, Mont Sinai Medical Center, USA. He has worked at Agricultural Biotechnology Research Center, Academia Sinica, Taiwan, from assistant research fellow to research fellow and deputy director. Currently, he serves as a research fellow and chief executive officer of Herbal Medicine Research Center, Academia Sinica, and a joint professor in the several local universities.

Dr. Yang ‘s research aims to promote human and animal health, including (1) the identification of diagnostic and therapeutic targets of diabetes, followed by R&D of anti-diabetic remedies for humans, (2) the identification of diagnostic and therapeutic targets of cancer, followed by R&D of anti-cancer remedies for humans and (3) R&D of phytogenics as in-feed additive and/or medicine for animal health. In this regard, he hopes to use phytogenics to enhance farm animal health.

His expertise covers agricultural biotechnology, natural products and medicine for human and animal health. Up to date, he published over 70 high impact papers in agriculture- and medicine-related journals. He is also an editorial board member and/or guest editor in chief in 8 international journals and a grant reviewer. Besides, he obtained 30 patents and 8 technology transfers with a total of 200 million Taiwan dollars. As a result, several products for human health and animal health were licensed and commercialized locally and internationally. For instance, Diabetter^® and Remeta-BP^® are herbal nutraceutical for diabetes, which have been on the market in Taiwan for 12 years. Both anti-diabetic products can manage and reverse diabetes via their unique mechanism that protects islet cells from death and dysfunction. PS1 is a first-in-class drug for treating and curing diabetes, developed by Pharmasaga Co. Ltd., and now is on clinical trial. Regarding functional phytogenics, Rotam-CS® and Altruism-CS® are two phytogenic formulations for coccidiosis and leukocytozoonosis in poultry. Of them, the Rotam-CS® was commercialized in Taiwan and abroad 6 years ago. EUBIO-Base® is another phytogenic formulation for bacterial infections in food animals and aquaculture. The mechanism of EUBIO-Base® is to decrease pathogenic bacteria and increases probiotics in the guts of animals. It is under field trial in Thailand and China. With these functional phytogenics, we hope to provide antibiotic-free solutions to food animals and aquaculture in compliance to One Health concept.

PROFESSIONAL EXPERIENCE

Livestock Research Institute, Taiwan

Associate researcher, Animal nutrition division

Developed functional feed additive for animal health care

Set-up of pilot type production plant of functional feed additive manufacture

Designed new tools and methodologies to evaluate active ingredients of microbial agents

Assistant researcher, Animal processing division

Developed functional ingredient of milk for animal product development

Assisted to manage operation of milk plant

Participated in Taiwan fresh milk mark and Certified Agricultural Standards mark certification work

Assistant researcher, R&D

Assisted to manage annual budget and research project of institute

EDUCATION

Ph.D., Animal Science, 2018- National Taiwan University

Dissertation: The study of Lactobacillus kefiranofaciens M1 and Lactobacillus mali APS1 on diet-induced obesity

M.S. Animal Science, 2008 - National Taiwan University

Thesis: Microencapsulated formulation of rhizobacteria Bacillus cereus C1L and application in fungal disease control

Research background

The underuse crop residues are getting more and more important, with various purposes including countermeasure to the Climate Change of the sustainable society, activation of rural area, and economic development of the country. So far, I have studied the bioethanol production from oil palm sap and cassava wastes by using of non-conventional yeasts. I pay the attention the cassava residues after ethanol fermentation as nutrient enrichment, and I examined to investigate the diverse functions on the cassava residues. I would like to show the novel function on the fermentable residues by combination of non-conventional yeast and cassava pulp through the investigation.

References

1. Julius E.N., A. Ndubuisi, Y. Murata, et al., Biofuels, 2019 p.1-10

Isolation and evaluation of xylose-fermenting thermotolerant yeasts for bioethanol production

2. C. O. Nwuche, Y.Murata, et al. Process Biochemistry.2018 67: 105-112

Bioethanol production under multiple stress condition by a new acid and temperature tolerant Saccharomyces cerevisiae strain LC 269108 isolated from rotten fruits

3. A. Ndubuisi, Y.Murata, et al. Energy and Power Engineering 2018 10(10): 457-474

Ethanol Production from Cassava Pulp by a Newly Isolated Thermotolerant Pichia kudriavzevii LC375240

4. Y. Murata, H. Danjarean, et al. AIMS Journal. 2015 3: 201-213

Ethanol fermentation by the thermotolerant yeast, Kluyveromyces marxianus TISTR5925, of extracted sap from old oil palm trunk

5. Y. Murata, S. kubo, et al. Industrial Crops and Products 2015 65 190-197

Detection of vascular bundles using cell wall birefringence on exposure to polarized light

Transcriptome analysis elucidation of stress response on Saccharomyces cerevisiae under several hostile conditions.

I evaluated the cellular responses of yeast through the expressed genes and metabolites, and showed the influence of environmental stress on S. cerevisiae.

Appl Biochem Biotech. 162:855, 2010; J Biosci Bioeng. 106:405, 2008; FEMS Microbiol Lett. 277:70, 2008; Yeast 24:901, 2007; FEMS Yeast Res.7:244, 2007; Chem-Bio Inf. J. 6:29, 2006; Extremophiles 10:117, 2006 (8 publications before 2006)

Rewards

For best presentation (2002) In The Chem-Bio Informatics Society　

For best article (2001) In The Chem-Bio Informatics Society

2000-2005 University of Saskatchewan BS

2005-2008 China Agricultural University MSc

2008-2011 China Agricultural University PhD

2012-2016 CJ Bio

2016-now J. John group and Calton Commercial Corporation

Present job:

Technical Specialist, Animal Farm Management Section, Department Of Animal Industry, Council Of Agriculture Executive Yuan, Taiwan, ROC (from2019/11)

Experience

Associate Technical Specialist, Livestock Industry Division, Animal Industry Department, Council Of Agriculture Executive Yuan, Taiwan, ROC(from2012/10 to 2019/11)

Associate Technical Specialist, Livestock Division, Department of Agriculture, Changhua County Government, Changhua, Taiwan, ROC (from 2008/10 to 2012/10)

Dr. Noraini Samat has more than 20 years of research experiences in feed biotechnology and bioprocessing, animal nutrition and livestock feeding. She graduated with a degree of Bachelor of Science (B.Sc. Hons) in Biotechnology from The University of Leeds, UK in 1996. In 1997, she joined MARDI at the Livestock Research Centre as a research officer. In 2011, she graduated from Universiti Kebangsaan Malaysia (UKM) with a degree of Master of Science (M.Sc.) in Microbiology before pursuing her PhD in Biology at the University of Leeds, UK, specialized in poultry nutrition.

Her research interests are on the improvement of the quality and digestion efficiency of low-quality feed and feed ingredients through the application of various technologies including fermentation, bioprocessing, feed processing, nanoscience and enzyme technology. To date, she had led more than 10 research projects and involved in several collaboration projects that required her contributions in poultry nutrition, fermentation, feed evaluation and analysis. She received MARDI Excellence Service Award in 2009, received more than 20 awards in national and international Science and Technology competitions, published over 50 refereed and non-refereed papers and had co-supervised over 10 final year degree, masters, PhD students and industrial training students from local universities.

Currently, she is the Deputy Director, Feed and Nutrition Program, Livestock Science Research Centre, MARDI. A member of several Technical Committees including Technical Committee of Malaysian Animal Feed Act

20+ years 1983-2004 In Novartis Animal Health As Sales & Marketing Director

8 +years 2004-2012 In Stallen International Ltd., As Executive Vice President

9 +years 2012-2021 In Rotam Animal Health As Business Head/ Vice General Manager Taiwan & Asian Exports

Experienced business Head with a demonstrated history of working in the pharmaceuticals industry. Skilled in Building team, Business management, Business planning, Marketing management, Veterinary Medicine, Market Planning , commercial operation, Sales management and Talent development

2015, Ph.D from National Cheng Kong University in chemical engineering

2015-2016, Foreign research fellow in ATRI

2016. Assisting in the development of Taiwan’s first canine cancer treatment drug for dogs(Antican®)

2016-2017, Research fellow in YEASTERN BIOTECH CO., LTD

2017, Established YoHoo co., LTD

2018, The first glucose stable supplies for animal was developed with mulberry leaf and guava leaf extracts, called “YoHoo Glucostead”.

2018-2019, Cooperated with the ATRI to jointly develop ATRiron (interferon, IFNs) for animals, which can be used to treat various immune diseases (such as FIV, FIP) and viral infectious diseases (such as FCov).

2019-2020, Developed an animal skin soothing cream formula by scutellaria and ginger flowers produced in Taiwan’s Hualien.

2021, Established new brand called “PlantAnimalCare®”, also called “PAC®”.

Dr. Chi holds Ph.D degree in Animal Nutrition from The Ohio State University, M.S. from University of California, Davis, and B.S. from National Taiwan University. He has near 30 years of Animal Nutrition industry experience and spent most of his time working in the US. His working experience ranging from a start-up company to a Fortune 500 organization, with various of functions including field technical services, product development, and sales and marketing responsibilities. Prior to join SYNBIO TECH Inc., Dr. Chi worked at Amlan International in Chicago; Renessen LLC (Cargill/Monsanto Joint Venture) in Deerfield, Illinois; Monsanto Company in St Louis Missouri; and APC Inc. in Ames, Iowa. Currently he works at SYNBIO, a Taiwanese company, as Chief Operating Office.

Director, R&D Division/International Business Division, Reber Genetics Co., Ltd

Lecturer of the International Degree Program of Animal Vaccine Technology, National Pingtung University of Science and Technology (NPUST), Taiwan.

Dr. Frank Chia-Jung Chang obtain his Ph.D. from Life Science and Institute of Genome Science department of Taiwan Yang-Ming Medical University in 2012. He received his postdoctoral training at Molecular Biology Institute of Academia Sinica (Taiwan) and worked as visiting scholar in Frances H. Arnold’s laboratory (Nobel Prize in Chemistry winner in 2018), Chemistry and Chemical Engineering Department of California Institute of Technology (U.S.). He joined Reber Genetics as the supervisor of R&D Division in 2014 and promoted to Senior Scientist in 2017. From 2017, Dr. Chang also works for business model development and strategic layout of vaccine products (technology included) in international market. Dr. Chang has considerable expertise in virology, reverse vaccinology, protein engineering and industrial production. He has several SCI papers, invention patents of protein engineering and his group has successfully developed the subunit vaccines of swine PRRSV, PCV2, PRRS+PCV2, CSFV, PEDV and porcine bacteria in Reber Genetics, furthermore, also has studied ASFV subunit vaccine with external partners.

Publications:

1. Duy Tien Do* , Khanh Doan Vinh Tran , Anh Tuyet Quach , David Lee , Frank CJ Chang , Carol PY Wu , Toan Nguyen Tat , Chanhee Chae. (2021) A comparative efficacy test of 1 versus 2 doses of CIRCOQ PCV2 subunit vaccine against naturally occurring PCV2d in piglets with high maternally derived antibodies (MDAs) on a Vietnamese swine farm. Can. J Vet Res. Apr;85(2):93-100.

2. Wu C.P., Chang CJ, Li CH, and Wu YL*. (2021) the Influence of Serial Passage on the Stability of an Exogenous Gene Expression in Recombinant Baculovirus. Entomol. Res.

3. Tsai CH, Wei SC, Jan JT, Liao, LL; Chang CJ; Chao, YC* (2019) Generation of Stable Influenza Virus Hemagglutinin through Structure-Guided Recombination. ACS Synth Biol. 8(11):2472-2482.

4. Chang CJ, Lee CC, Chang YT, Trudeau DL, Wu MH, Tsai CH, Yu SM, Ho T-HD, Wang AH, Hsiao CD, Arnold FH and Chao YC* (2016) Exploring the Mechanism Responsible for Cellulase Thermostability by Structure-Guided Recombination. PLOS ONE. 11(3): e0147485

5. Wu YL, Wu CP, Huang YH, Huang SP, Lo HR, Chang HS, Lin PH, Wu MC, Chang CJ, and Chao YC* (2014) Identification of a High-efficiency Baculovirus DNA Replication Origin that Functions in Insect and Mammalian Cells. J. Virol. 88(22), 13073-13085

6. Chang CJ, Wu CP, Lu SC, Chao A, Ho T-HD, Yu SM, and Chao YC* (2012) A Novel Exo-cellulase from White Spotted Longhorn Beetle (Anoplophora malasiaca). Insect Biochem. Mol. Biol. 42, 629-636.