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Problem Definition
A singular device that can apply pressure, shear stress, and cyclical stretching onto cells did not exist in order for the Experimental Biofluidics Lab at UCSD to be able to study the genetic expression of cells under biomechanical forces.
Proposed Design Solution
An enclosed cell stretcher that has 5 main components:
A flexible cell membrane that cultured cells can be adhered to during testing
A cell chamber that follows the parameters needed to create a Hele-Shaw flow while holding for 1 Hele-Shaw cellÂ
A pressure seal assembly to ensure that the device is seal-tight during testing
An actuator system with a pump to drive the flow to apply shear stress onto the cells and a motor to drive the cyclical stretching of the membrane
A control system that sets the parameters for the cyclical stretching and records the desired variables: temperature, pressure, flow rate, and cylic stretching
Top-View Image of the Cell Stretcher
Prototype Performance
Holds pressure for low flow
Allows user to visibility detect for leaks with the top plate
Measures and records temperature, pressure, and flow rate
Motor assembly provides necessary torque to meet uniaxial stretching requirements
Membrane capable of reaching above 20% stretching deformation without fracture
Complexity of assembly leads to concerns for lab storage
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