The final system is a self-contained, portable perfusion platform built around five core subsystems: a peristaltic pump, a pediatric oxygenator and heat exchanger, a PID-controlled hot-water heating loop, an in-line sensor and control suite, and an organ chamber. A custom Teensy 4.1 PCB consolidates all sensing and control, including dual PT100 temperature probes, an ultrasonic flow sensor, and a medical-grade pressure transducer, and drives both the pump and heater over the same board. A browser-based GUI provides live telemetry, alarms, and CSV logging, while an aluminum T-slot enclosure keeps the disposable fluid path up front for quick access. Two complete devices were built and delivered to the sponsors.

The integrated system was validated on a water loop and an extended human-kidney perfusion. Pump flow was controllable across the operating range over RS-485 Modbus, the pressure channel resolved downstream resistance changes up to 400 mmHg (well beyond the 65 to 95 mmHg operating window), and the heating system held the perfusate within ±0.2 °C of the 34 to 37 °C setpoint under closed-loop PID control. In a 100-minute perfusion of a discarded human kidney, flow held at a ~107 mL/min mean and mean arterial pressure stayed in the ~90 to 103 mmHg range, with the organ visibly transitioning from pale to uniformly reperfused. Two complete devices were delivered to the sponsors.