Design Choices
Design Choices
Embedded Files
Alt. Design 1
Alt. Design 1
Single Image Quantification
Single Image Quantification
- Capture single still image of purified HIV sample
- Count binary pixels for quantification
Alt. Design 2
Alt. Design 2
FLIM w/ Camera Detector
FLIM w/ Camera Detector
- Use Raspberry Pi camera as lens and storage
- 3D printed box and stage
- Utilize Lifetime Fluorescent Image
Alt. Design 3
Alt. Design 3
Flow Cytometry
Flow Cytometry
- Individually count number of HIV cells from a purified solution during PCR
Design Decision Evaluation
Design Decision Evaluation
Current Imager Design
Current Imager Design
Quantification Method
Quantification Method
1. Fluorescent Imaging
1. Fluorescent Imaging
- Excites samples with blue LED
- Captures and stores series of images of the excited fluoroscope attached to HIV RNA
2. Threshold
2. Threshold
- Convert raw images to gray-scale
- Calculate average intensity of each image
- Plot intensity over time
3. Measure
3. Measure
- Calculate time constant of fluorescent decay
- Find original yield of HIV RNA using the decay time constant and intrinsic lifetime of the fluorophore
Results
Results
Current prototype is able to:
Current prototype is able to:
- Detect fluorescence on the millimeter scale
- Capture and store still fluorescent images under high resolution
- Accurately compute average intensity over a fixed volume
...all under $300
...all under $300
Group 7- Low-Cost Fluorescent ImagerDepartment of BioengineeringUniversity of California, San DiegoBENG 187 - Bioengineering Design Project
Page Editor: Valerie Porter
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