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Design Solution
Design Solution
The development of a 3D printed micro/millifluidic insert compatible with existing well plates/microscopes that is coupled to a single board computer controlled pumping system, leading to the creation of a microbioreactor. The proposed system will be able to deliver predetermined volumes of new media into each of 24 wells of the plate to feed the developing stem cells slowly every couple of hours in addition to aspirating used up media from the wells.
Evolution of our system from Version 1 to Version 2.
This image shows the first half of our system that primarily handles dispensing chilled media to cell cultures. The dispensing system includes a fridge with temperature control to keep feeding media at 4-10°C, a Peristaltic pump to move the fluids, and a customized mini-incubator.
This image contains the second half of our system, dealing with aspirating and monitoring cell waste. The aspirating system primarily contains an air pump, two vacuum traps, and a pH monitoring system controlled by an Arduino nano. Both parts are controlled by a Raspberry Pi and a display monitor.
Inner channel designs of our insert with longer pipes dispense media while shorter pipes aspirate waste
6-well plate implementation
24-well plate implementation
Editor: Meghna Bahry
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