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Purpose
Purpose
Our projects aims to optimize methods of screening cardiomyocytes -- specifically, human induced pluripotent stem cell derived cardiomyocytes with the ACEA Biosciences xCELLigence CardioECR RTCA machine, but with a flexible enough approach for any workflow.
Highlights
Highlights
- Comparing performance and analyzing costs of various substrates
- Designing and manufacturing a PDMS stamp to pattern cells in order to match true myocardium structure
- Validating a wash step for reusing plates in order to decrease cost and environmental impact
Image Source: McDevitt et. al
Problem Statement
Problem Statement
Current methods of screening mechanical and electrical function in cardiomyocytes are of insufficient throughput due to:
- the high cost of the assay plates,
- the long cell seeding and recovery times, and
- the limitation of single-use assay plates.
Total cost of plates alone for screening a 10,000 compound library, depending on amount of uses per plate. Assumed 6 compounds per plate to account for controls and dilutions.
Cost per well of coating with various substrates. PBS for dilution from stock not included.
Senior Design Video
Senior Design Video
Senior Design.mp4The video link: https://drive.google.com/open?id=1GiYd4l2nltDueII2apGHtSmSmVRarR4a
Page Leader: Natalie Tetreault
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