[*Corresponding Author/Co-corresponding Author]
Liu, T.Y., Yan, J.J., Guh, Y.J., Hayasaka, O., Lin, L.Y., Hwang, P.P., Wu, G.C., Chung, M.T., Tseng, Y.C.* 2025. Epigenetic insights into physilogical resilience: multigenerational readouts of CO2-induced seawater acidification effects on fish embryos. iScience 28, 113187. (doi: 10.1016/j.isci.2025.113187)
Chiu, L., Guo, J.L., Li, H.W., Chang, H.J., Yang, S.H., Dufour, S., Chang, C.F., Tseng, Y.C.*, Wu, G.C.* 2025. Microbial diversity and pigment synthesis in the accessory nidamental gland: Species-specific and color-associated patterns in bigfin reef squid (Sepioteuthis lessoniana). Anim. Microbiome 7, 36. (doi: 10.1186/s42523-025-00402-2)
Hsieh, C.Y., Liu, T.Y., Tseng, Y.C., Shirai, K., Wang, P.L., Wu, G.C., Chung, M.T.* 2025. Estimation of lifelong metabolic rates in marine fish: A combination of oxygen consumption measurements and δ13C metabolic proxy derived from vertebral structural carbonates. Limnol. Oceanogr. Lett. 10, 403-411. (doi: 10.5061/dryad.bnzs7h4jx)
Lee, Y.C., Tang, L.L., Yeh, Y.C., Li, H.W., Ke, H.M., Yang, C.J., Chinag, C.I., Wang, C.H., Tseng, Y.C., Chang, C.F., Tsai, I.J.*, Wu, G.C.* 2025. Detection of genetic sex using a qPCR-based method in the bigfin reef squid (Sepioteuthis lessoniana). Aquac. Rep. 40, 102644. (d0i: 10.1016/j.aqrep.2025.102644)
Wang, C.S., Cheng, H.S., Chang, W.T., Hsiao, C.C., Tseng, P.W., Li, H.W., Sagi, A., Chang, C.F., and Wu, G.C.* 2025. Characterization of a novel and testis-specific zinc finger protein during sexual development of Pacific white shrimp Litopenaeus vannamei. Biol. Reprod. 112, 179-190. (doi: 10.1093/biolre/ioae151)
Chuang, H.J., Chiu, L., Liao, B.J., Chang, C.Y., Wu, G.C., Tseng, Y.C., Chou, M.Y., and Hwang, P.P.* 2024. Environmental acidification drives inter-organ energy mobilization to enhance reproductive performance in medaka (Oryzias latipes). J. Hazard. Mater. 480, 136197. (doi: 10.1016/j.jhazmat.2024.136197)
Jeng, S.R.*, Wu, G.C.*, Yueh, W.S., Liu, P.H., Kuo, S.F., Dufour, S., and Chang, C.F.* 2024. The expression profiles of cyp19a1, sf-1, esrs and gths in the brain-pituitary during gonadal sex differentiation in juvenile Japanese eels. Gen. Comp. Endocrinol. 352, 114521. (doi: 10.1016/j.ygcen.2024.114512)
Tseng, P.W., Lin, C.J., Tsao, Y.H., Kuo, W.L., Chen, H.C., Dufour, S., Wu, G.C.*, and Chang, C.F.* 2024. The effect of gonadal hormones on the gene expression of brain-pituitary in protandrous black porgy, Acanthopagrus schlegelii. Gen. Comp. Endocrinol. 351, 114428. (doi: 10.1016/j.ygcen.2024.114482)
Chen, C., Wu, G.C.*, Chung, Y.T., Li, H.W., Chou, P.H., Tseng, Y.C.*, Chen, C.J., Dufour, S., and Chang, C.F.* 2023. Duplicated paralog of sulfide: Quinone oxidoreductase contributes to the adaptation to hydrogen sulfide-rich environment in the hydrothermal vent crab, Xenograpsus testudinatus. Sci. Total Environ. 890, 164257.
Chou, P.H., Hu, M.Y., Guh, Y.J., Wu, G.C., Yang, S.H., Tandon, K., Shao, Y.T., Lin, L.Y., Chen, C., Tseng, K.Y., Wang, M.C., Zhang, C.M., Han, B.C., Lin, C.C., Tang, S.L., Jeng, M.S., Chang, C.F., and Tseng, Y.C.* 2023. Cellular mechanisms underlying extraordinary sulfide tolerance in a crustacean holobiont from hydrothermal vents. Proc. R. Soc. B. 290, 20221973.
Tseng, P. W., Li, H. W., Chen, C., Tseng, Y. C., Chang, C. F., and Wu, G. C.* 2023. Transcriptomic Profile of Symbiotic Accessory Nidamental Gland During Female Maturation in Bigfin Reef Squid. Front. Mar. Sci. 9, 1026742.
Kuan, P. L., You, J. Y., Wu, G. C.*, and Tseng, Y. C.* 2022. Temperature increases induce metabolic adjustments in the early developmental stages of bigfin reef squid (Sepioteuthis lessoniana). Sci. Total Environ. 844, 156962.
Li, H. W., Chen, C., Kuo, W. L., Tseng, Y. C., Chang, C. F., and Wu, G. C.* 2022. The characteristics and expression profile of peptidoglycan recognition protein 2 in the accessory nidamental gland of the bigfin reef squid during bacterial colonization. Front. Mar. Sci. 9, 825267.
Tseng, P. W., Wu, G. C.*, Kuo, W. L., Tseng, Y. C.*, and Chang, C. F.* 2022. The ovarian transcriptome at the early stage of testis removal-induced male-to-female sex change in the protandrous black porgy Acanthopagrus schlegelii. Front. Genet. 13, 816955.
Lin, C. J., Jeng, S. R.*, Lei, Z. Y., Yueh, W. S., Dufour, S., Wu, G. C.*, and Chang, C. F.* 2021. Involvement of transforming growth factor beta family genes in gonadal differentiation in Japanese eel, Anguilla japonica, according to sex-related gene expressions. Cells 10, 3007.
Yang, S. H., Chen, C., Hsieh, Y., Yang, S. Y., Li, H. W., Ching, T. Y., Wang, C. H., Chang, C. F., Tang, S. L.*, and Wu, G. C.* 2021. Bacterial dynamics in the accessory nidamental gland of Sepioteuthis lessoniana throughout maturation. Microbes Environ. 36, ME21030.
Guo, C. Y., Tseng, P. W., Hwang, J. S., Wu, G. C.*, and Chang, C. F.* 2021. Potential role of DNA methylation of cyp19a1a promoter during sex change in protogynous orange-spotted grouper, Epinephelus coioides. Gen. Comp. Endocrinol. 311, 113840. (SCI)
Guo, C. Y., Tseng, P. W., Hwang, J. S., Wu, G. C.*, and Chang, C. F.* 2021. The characteristics and expression profile of gonadal soma-derived factor (gsdf/Gsdf) during androgen-induced reversible sex change in the protogynous orange-spotted grouper, Epinephelus coioides. Aquaculture 542, 736832.
Wu, G. C., Dufour, S., and Chang, C. F.* 2021. Molecular and cellular regulation on sex change in hermaphroditic fish, with a special focus on protandrous black porgy, Acanthopagrus schlegelii. Mol. Cell. Endocrinol. 520, 111069. (Invited article)
Wang, M. C., Wang, Y. C., Peng, H. M., Hseu, J, R., Wu, G. C., Chang, C. F., and Tseng, Y. C.* 2020. Resveratrol induces expression of metabolic and antioxidant machinery and protects tilapia under cold stress. Int. J. Mol. Sci. 21, 3338.
Lin, C. J., Maugars, G., Lafont, A. G., Jeng, S. R., Wu, G. C., Dufour, S.*, and Chang, C. F.* 2020. Basal teleosts provide new insights into the evolutionary history of teleost-duplicated aromatase. Gen. Comp. Endocrinol. 291, 113395.
Li, H. W., Chen, C., Kuo, W. L., Lin, C. J., Chang, C. F., and Wu, G. C.* 2019. The characteristics and expression profile of transferrin in the accessory nidamental gland of the bigfin reef squid during bacteria transmission. Sci. Rep. 9, 20163.
Lin, C. J., Wu, G. C., Dufour, S.*, and Chang, C. F.* 2019. Activation of the brain-pituitary-gonadotropic axis in the black porgy Acanthopagrus schlegelii during gonadal differentiation and testis development and effect of estradiol treatment. Gen. Comp. Endocrinol. 281, 17-29.
Jeng, S. R., Wu, G. C.*, Yueh, W. S., Kuo, S. F., Dufour, S., and Chang, C. F.* 2019. Dmrt1 (doublesex and mab-3-related transcription factor 1) expression during gonadal development and spermatogenesis in the Japanese eel. Gen. Comp. Endocrinol. 279, 154-163.
Wu, G. C.*, Jeng, S. R., Pan, Y. T., Li, H. W., Kuo, W. L., Lin, C. J., and Chang, C. F.* 2019. The germline-specific expression of Foxl3a and its paralogous Foxl3b are associated with the male gonadal differentiation in the Japanese eel, Anguilla japonica. Gen. Comp. Endocrinol. 277, 56-65.
Chen, C., Li, H. W., Kuo, W. L., Lin, C. L., Chang, C. F., and Wu, G. C.* 2018. Two distinct vitellogenin genes are similar in function and expression in the bigfin reef squid, Sepioteuthis lessoniana. Biol. Reprod. 99, 1034-1044.
Wu, G. C.* and Chang, C. F.* 2018. Primary males guide the femaleness through the regulation of testicular Dmrt1 and ovarian Cyp19a1a in protandrous black porgy. Gen. Comp. Endocrinol. 261, 198-202.
Jeng, S. R.*, Wu, G. C.*, Yueh, W. S., Kuo, S. F., Dufour S., and Chang, C. F.* 2018. Gonadal development and expression of sex-specific genes during sex differentiation in the Japanese eel. Gen. Comp. Endocrinol. 257, 74-85.
Wu, G. C.*, Luo, J. W., Li, H. W., Huang, C. H., and Chang, C. F.* 2017. Robust gdf9 and bmp15 expression in the oocytes of ovotestes through the Figla-independent pathway in the hermaphroditic black porgy, Acanthopagrus schlegelii. PLoS ONE 12(10): e0186991.
Wu, G. C.*, Li, H. W., Tey, W. G., Lin, C. J., and Chang, C. F.* 2017. Expression profile of amh/Amh during bi-directional sex change in the protogynous orange-spotted grouper Epinephelus coioides. PLoS ONE 12(10): e0185864.
Wu, G. C.*, Li, H. W., Huang, C. H., Lin, H. J., Lin, C. J., and Chang, C. F.* 2016. The testis is a primary factor that contributes to epigenetic modifications in the ovaries of the protandrous black porgy, Acanthopagrus schlegelii. Biol. Reprod. 94: 132, 1-13.
Wu, G. C.*, Trey, W. G., Li, H. W., and Chang, C. F.* 2015. Sexual fate reprogramming in the steroid-induced bi-directional sex change in the protogynous orange-spotted grouper, Epinephelus coioide. PLoS ONE 10(12): e0145438.
Wu, G. C.*, Li, H. W., Luo, J. W., Chen, C., and Chang, C. F.* 2015. The potential role of Amh to prevent ectopic female development in testicular tissue of the protandrous black porgy, Acanthopagrus schlegelii. Biol. Reprod. 92: 158, 1-13.
Chen, M., Lu, Y. L., Sio, C. P., Wu, G. C., Tzou, W. S., Pai, T. W.* 2014. Gene ontology based housekeeping gene selection for RNA-seq normalization. Method 67, 354-363.
Riviere, G.*, Wu, G. C., Fellous, A., Goux, D., Sourdaine, P., Favrel, P. 2013. DNA methylation is crucial for the early development in the oyster C. gigas. Mar. Biotechnol. 15, 739- 53.
Wu, G. C.* and Chang, C. F.* 2013. Oocytes survive in the testis by altering the soma fate from male to female in the protandrous black porgy, Acanthopagrus schlegeli. Biol. Reprod. 88: 19, 1-10.
Wu, G. C.* and Chang, C. F.* 2013. The switch of secondary sex determination in protandrous black porgy, Acanthopagrus schlegeli. Fish Physiol. Biochem. 39, 33-38.
Wu, G. C., Chiu, P. C., Lin, C. J., Lyu, Y. S., Lan, D. S., Chang, C. F.* 2012. Testicular dmrt1 is involved in the sexual fate of the ovotestis in the protandrous black porgy. Biol. Reprod. 86: 41, 1-11.
Wu, G. C., Chiu, P. C., Lyu, Y. S., and Chang, C. F.* 2010. The expression of amh and amhr2 is associated with the development of gonadal tissue and sex change in the protandrous black porgy, Acanthopagrus schlegeli. Biol. Reprod. 83, 443-453.
Lin, C. J., Wu, G. C., Lee, M. F., Lau, E. L., Dufour, S., and Chang, C. F.* 2010. Regulation of two forms of gonadotropin-releasing hormone receptor gene expression in the protandrous black porgy fish, Acanthopagrus schlegeli. Mol. Cell. Endocrinol. 323, 137-146.
Wu, G. C., Tomy, S., Lee, Y. H., Lee, M. F., Yueh, W. S., Lin, C. J., Lau, E. L. and Chang, C. F.* 2010. Sex differentiation and sex change in the protandrous black porgy, Acanthopagrus schlegeli. Gen. Comp. Endocrinol. 167, 417-421.
Wu, G. C. and Chang, C. F.* 2009. Wnt4 is associated with the development of ovarian tissue in the protandrous black porgy, Acanthopagrus schlegeli. Biol. Reprod. 81, 1073-1082.
Tomy, S., Wu, G. C., Huang, H. R. and Chang, C. F.* 2009. Age-dependent differential expression of genes involved in steroid signalling pathway in the brain of protandrous black porgy, Acanthopagrus schlegeli. Dev. Neurobiol. 69, 299-313.
Wu, G. C., Tomy, S., Nakamura, M., and Chang, C. F.* 2008. Dual roles of cyp19a1a in gonadal sex differentiation and development in the protandrous black porgy, Acanthopagrus schlegeli. Biol. Reprod. 79, 1111-1120.
Wu, G. C., Tomy, S., and Chang, C. F.* 2008. The expression of nr0b1 and nr5a4 during gonad development and sex change in protandrous black porgy fish, Acanthopagrus schlegeli. Biol. Reprod. 78, 200-210.
Tomy, S., Wu, G. C., Huang, H. R., Dufour, S. and Chang, C. F.* 2007. Developmental expression of key steroidogenic enzymes in the brain of protandrous black porgy fish, Acanthopagrus schlegeli. J. Neuroendocrinol. 19, 643-655.
Wu, G. C., Du, J. L., Lee, Y. H., Lee, M. F., and Chang, C. F.* 2005. Current status of genetic and endocrine factors in the sex change of protandrous black porgy, Acanthopagrus schlegeli. Annals New York Academy Sci. 1040, 206-214.
Lee, Y. H., Wu, G. C., Du, J. L., and Chang, C. F.* 2004. Estradiol-17b induced a reversible sex change in the fingerlings of protandrous black porgy, Acanthopagrus schlegeli Bleeker. Biol. Reprod. 71, 1270-1278.
He, C. L., Du, J. L., Wu, G. C., Lee, Y. H., Sun, L. T., and Chang, C. F.* 2003. Differential Dmrt1 transcripts in gonads of the protandrous black porgy, Acanthoparus schlegeli. Cytogenet. Genome Res. 101, 309-313.
1. Chou, H. Y. and Wu, G. C. 2021. Aquaculture and Fisheries. A chapter in "The Physiology of Fishes: Fifth edition". Suzanne Currie and David H. Evans (eds). Chapter 15, 203-212. CRC Press, Taylor & Francis Group. ISBN: 978-0-367-47755-4 (Book's chapter)