Si-Yu Li

Li, Si-Yu

Professor of Chemical Engineering Dept.

National Chung Hsing University

145 Xingda Rd., Taichung City 40227, Taiwan

Phone: 886-4-2284-0510 Ext.509

E-mail: syli@dragon.nchu.edu.tw

EDUCATION

2006-2010 Ph.D. in Chemical Engineering, University of Connecticut, USA

2003-2005 M.S. in Chemical Engineering, National Chung Hsing University, Taiwan

1999-2003 B.S. in Chemical Engineering, National Chung Hsing University, Taiwan

PROFESSIONAL EXPERIENCE

2019 - Present Professor, Department of Chemical Engineering, National Chung Hsing University, Taichung, Taiwan

2017- 2021 Director of Division of Project Affairs, Office of Research and Development, National Chung Hsing University, Taiwan

2015 - 2019 Associate Professor, Department of Chemical Engineering, National Chung Hsing University, Taichung, Taiwan

Research interests：CO₂ utilization; Metabolic Engineering; Microbial Physiology

2011 - 2015 Assistant Professor, Department of Chemical Engineering, National Chung Hsing University, Taichung, Taiwan

RESEARCH INTERESTS

CO₂ conversion; Fermentative biopolymer production

45. Zhuo, X.-z., Chou, S.-C. & Li, S.-Y.^* (submitted) Deregulating unsaturated fatty acid biosynthesis by FabR induction increases medium-chain-length polyhydroxyalkanoate production in Escherichia coli.

44. Liu, Y.-X., Zhuo, X.-z., & Li, S.-Y.^* (accepted) The transcription activator AtxA from Bacillus anthracis was employed for developing a tight-control, high-level, modulable, and stationary-phase specific transcription activity in Escherichia coli. Synthetic Biology

43. Chou, S.-C., Lai, Y.-J., Zhuo, X.-z., Chen, W.-Y.^*, & Li, S.-Y.^* (accepted) Increasing the l-Red mediated gene deletion efficiency in Escherichia coli using methyl phosphotriester-modified DNA. Journal of the Taiwan Institute of Chemical Engineers.

42. Liao, Y.-C., Saengsawang, S., Chen, J.-W., Zhuo, Xiao-zhen, & Li, S.-Y.^* (2022) Construction of antibiotic-free vector and its application in metabolic engineering of Escherichia coli. Frontiers in Bioengineering and Biotechnology. 10: 837944.

41. Chang, Y.-C., Sil M., Y.-P. Zhang, Chou, S.-C., Liu, Y.-X., Li, S.-Y.^* & C.-M. Chen^* (2022) Surface co-silanization engineering to enhance amine functionality for adhesive heterojunction and antimicrobial application. Composites Science and Technology, 218, 109196.

40. Chang, P.-Y., Wang, J., Li, S.-Y.^* & Suen, S.-Y.^* (2021) Biodegradable polymeric membranes for organic solvent/water pervaporation applications. Membranes, 11, 970.

39. Hu, N.-J., Li, S.-Y., & Liu, Y.-C.^* (2021) Recent Advances in Biocatalysis and Metabolic Engineering. Catalysts, 11, 1052.

38. Lo, S.-C., Chiang, E.-P. Isabel, Yang, Y.-T., Li, S.-Y., Peng, J.-H., Tsai, S.-Y., Wu, D.-Y., Yu. C.-H., Huang, C.-H., Su, T.-T., Tsuge, Kenji, & Huang, C.-C. (2021) Growth enhancement facilitated by gaseous CO₂ through heterologous expression of reductive tricarboxylic acid cycle genes in Escherichia coli. Fermentation, 7, 98.

37. Pang, J.-J., Shin, J.-S., & Li, S.-Y.^* (2020) The catalytic role of RuBisCO for in situ CO₂ recycling in Escherichia coli. Frontiers in Bioengineering and Biotechnology, 8:543807.

36. Liu, E.-J., Tseng, I-T., Chen, Y.-L., Shen, Z.-X., Pang, J.-J., & Li, S.-Y.^* (2020) The physiological responses of Escherichia coli triggered by phosphoribulokinase (PrkA) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). Microorganisms, 8, 1187.

35. Kuo, T.-C., Wu, M.-W., Lin, W.-C., Matulis, D., Yang, Y.-S., Li, S.-Y., & Chen, W.-Y.^* (2020) Reduction of Interstrand Charge Repulsion of DNA Duplexes by Salts and by Neutral Phosphotriesters-Contrary Effects for Harnessing Duplex Formation. Journal of the Taiwan Institute of Chemical Engineers, 110, 1-7. 台灣化學工程學會110年傑出論文獎

34. Fang, Y.-T., Li, S.-Y., Hu, N.-J., Liu, J.-H.^*, & Liu, Y.-C.^* (2020) Study on cecropin B2 production via construct bearing intein oligopeptide cleavage variants. Molecules, 25, 1005.

33. Chen, C.-H., Tseng, I-T., Lo, S.-C., Yu, Z.-R., Pang, J.-J., Yu, C.-H., Huang, C.-H., Wu, D.-Y., Chen, Y.-H., Huang, C.-C., & Li, S.-Y.^* (2020) Manipulating ATP supply improves in situ CO₂ recycling in engineered Escherichia coli. 3 Biotech, 10:125.

32. Chang, P.-H., Li, S.-Y., Juang, T.-Y.^*, & Liu, Y.-C.^* (2019) Mg-Fe layered double hydroxides enhance surfactin production in bacterial cells. Crystals, 9, 355.

31. Tseng, I-T., Chen, Y.-L., Chen, C.-H., Shen, Z.-X., Yang, C.-H., & Li, S.-Y.^* (2018) Exceeding the theoretical fermentation yield in mixotrophic Rubisco-based engineered Escherichia coli. Metabolic Engineering, 47, 445-452.

30. Li, S.-Y., Ng, I-S., Chen, P.-T., Chiang, C.-J.^*, & Chao, Y.-P.^* (2018) Biorefining of protein waste for production of sustainable fuels and chemicals. Biotechnology for Biofuels, 11, 256. (Highly accessed article in Biotechnology for Biofuels)

29. Chiang, Y.-S., Kuo, Y.-Y., Li, S.-Y.^* (2018) A novel method for preparing high purity Actinobacillus succinogenesstock and its long-term acid production in a packed bed reactor. Bioresource Technology Reports, 2, 62-68.

28. Hsiao, L.-J., Lee, M.-C., Chuang, P.-J., Lin, J.-H., Wu, T.-M., & Li, S.-Y.^* (2018). The production of poly(3-hydroxybutyrate) by thermophilic Caldimonas manganoxidans from glycerol. Journal of Polymer Research, 25, 85.

27. Lee, M.-C., Liu, E.-J., Yang, C.-H., Hsiao, L.-J., Wu, T.-M., & Li, S.-Y.^* (2018). Co-expression of ORF_Cma with PHB depolymerase (PhaZ_Cma) in Escherichia coli induces efficient whole-cell biodegradation of polyesters. Biotechnology Journal, 13, 1700560. (selected as Inside Back Cover)

26. He, C.-R., Lee, M.-C., Kuo, Y.-Y., Wu, T.-M., & Li, S.-Y.^* (2017) The influence of support structures on cell immobilization and Acetone-Butanol-Ethanol (ABE) fermentation performance. Journal of the Taiwan Institute of Chemical Engineers, 78, 27-31.

25. He, C.-R., Kuo, Y.-Y., & Li, S.-Y.^* (2017) Lignocellulosic butanol production from Napier grass using semi-simultaneous saccharification fermentation. Bioresource Technology, 231, 101-108.

24. He, C.-R., Huang, C.-L., Lai, Y.-C., & Li, S.-Y.^* (2017) The utilization of sweet potato vines as carbon sources for fermenting bio-butanol. Journal of the Taiwan Institute of Chemical Engineers, 79, 7-13.

23. Lin, J.-H., Lee, M.-C., Sue, Y.-S., Liu, Y.-C., & Li, S.-Y.^* (2017) Cloning of phaCAB genes from thermophilic Caldimonas manganoxidans in Escherichia coli for poly(3-hydroxybutyrate) (PHB) production. Applied Microbiology and Biotechnology, 101, 6419-6430.

22. Zeng, H.-S., He, C.-R., Yen, Andy T.-C., Wu, T.-M., & Li, S.-Y.^* (2017) Assessment of acidified fibrous immobilization materials for improving ABE fermentation. Fermentation (Feature Paper), 3:3.

21. Hsiao, L.-J., Lin, J.-H., Sankatumvong, P., Wu, T.-M., & Li, S.-Y.^* (2016) The feasibility of thermophilic Caldimonas manganoxidans as a platform for efficient PHB production. Applied Biochemistry and Biotechnology, 180, 852-871.

20. Wang, Y.-R., Chiang, Y.-S., Chuang, P.-J., Chao, Y.-P., & Li, S.-Y.^* (2016) Direct in situ butanol recovery inside the packed bed during continuous acetone-butanol-ethanol (ABE) fermentation. Applied Microbiology and Biotechnology, 100, 7449-7456.

19. Song, H. He, C.-R., Basdeo, C., Li, J.-Q., Ye, D., Kalonia, D., Li, S.-Y., & Fan, T.-H.^* (2016) Evaporation of binary mixtures and precision measurement by crystal resonator. International Journal of Heat and Mass Transfer, 100, 800-809.

18. Yang, C.-H., Liu, E.-J., Chen, Y.-L., Ou-Yang, F.-Y., & Li, S.-Y.^* (2016) The comprehensive profile of fermentation products during in situ CO₂ recycling by Rubisco-based engineered Escherichia coli. Microbial Cell Factories, 15:133.

17. Li, S.-Y., Chiang, C.-J., Tseng, I-T., He, C.-R., & Chao, Y.-P.^* (2016) Bioreactors and in situ product recovery techniques for acetone-butanol-ethanol fermentation. FEMS Microbiology Letters, 363 (13).

16. Saini, M.; Li, S.-Y., Wang, Z.-W., Chiang, C.-J.^*, & Chao, Y.-P.^* (2016) Systematic engineering of the central metabolism in Escherichia coli for effective production of n-butanol. Biotechnology for Biofuels, 9, 69.

15. Lu, K.-M., Chiang, Y.-S., Wang, Y.-R., Chein, R.-Y., & Li, S.-Y.^* (2016). Performance of fed-batch acetone–butanol–ethanol (ABE) fermentation coupled with the integrated in situ extraction-gas stripping process and the fractional condensation. Journal of the Taiwan Institute of Chemical Engineers, 60, 119-123.

14. Saini, M., Chiang, C.-J.^*, Li, S.-Y., & Chao, Y.-P.^* (2016) Production of biobutanol from cellulose hydrolysate by the Escherichia coli co-culture system. FEMS Microbiology Letters, 363(4).

13. Li, Y.-H., Ou-Yang, F.-Y., Yang, C.-H., & Li, S.-Y.^* (2015). The coupling of glycolysis and the Rubisco-based pathway through the non-oxidative pentose phosphate pathway to achieve low carbon dioxide emission fermentation. Bioresource Technology, 187, 189-197.

12. Lu, K.-M. & Li, S.-Y.^* (2014). An integrated in situ extraction-gas stripping process for Acetone-Butanol-Ethanol (ABE) fermentation. Journal of the Taiwan Institute of Chemical Engineers, 45, 2106-2110.

11. Liao, Y.-C., Lu, K.-M., & Li, S.-Y.^* (2014). Process parameters for operating 1-butanol gas stripping in a fermentor. Journal of Bioscience and Bioengineering, 118, 558-564.

10. Ciou, C.-Y., Li, S.-Y., & Wu, T.-M.* (2014). Morphology and degradation behavior of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/layered double hydroxides composites. European Polymer Journal, 59, 136-43.

9. Ho, M.-H., Li, S.-Y., Ciou, C.-Y., & Wu, T.-M.^* (2014). The morphology and degradation behavior of electrospun poly(3-hydroxybutyrate)/magnetite and poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/magnetite composites. Journal of Applied Polymer Science, 131, 41070.

8. Chen, S.-K., Chin, W.-C., Tsuge, K., Huang, C.-C., & Li, S.-Y.^* (2013). Fermentation approach for enhancing 1-butanol production using engineered butanologenic Escherichia coli. Bioresource Technology, 145, 204-209.

7. Zhuang, Z.-Y. & Li, S.-Y.^* (2013). Rubisco-based engineered Escherichia coli for in situ carbon dioxide recycling. Bioresource Technology, 150, 79-88.

6. Li, S.-Y., Srivastava, R., Suib, S.L., Li, Y., & Parnas, R.S.^* (2011). Performance of batch, fed-batch, and continuous A-B-E fermentation with pH-control. Bioresource Technology, 102, 4241-50.

5. Li, S.-Y., Srivastava, R., & Parnas, R.S.^* (2011). Study of in situ 1-butanol pervaporation from A-B-E fermentation using a PDMS composite membrane: Validity of solution-diffusion model for pervaporative A-B-E fermentation. Biotechnology Progress, 27, 111-20.

4. Li, S.-Y., Srivastava, R., & Parnas, R.S.^* (2010). Separation of 1-butanol by pervaporation using a novel tri-layer PDMS composite membrane. Journal of Membrane Science, 363, 287-94.

3. Li, S.-Y., Stuart, J.D., Li, Y., & Parnas, R.S.^* (2010). The feasibility of converting industrial hemp oil into biodiesel. Bioresource Technology, 101, 8457-60.

2. Li, S.-Y., Chang, B.-Y., & Lin, S.-C.^* (2006). Coexpression of TorD enhances the transport of GFP via the TAT pathway. Journal of Biotechnology, 122, 412-21.

1. Ho, L.-F., Li, S.-Y., Lin, S.-C.^*, & Hsu, W.-H. (2004). Integrated enzyme purification and immobilization processes with immobilized metal affinity adsorbents. Process Biochemistry, 39, 1573-1581.