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View under microscope (1000X magnification).
Next, a solution called Gram's iodine is poured over the slide. This will form large complexes with the crystal violet inside the bacteria. Some, but not all, bacteria will retain these purple complexes throughout the rest of the Gram stain procedure. Gram's iodine is also called the "mordant."
Next, a solution called Gram's iodine is poured over the slide. This will form large complexes with the crystal violet inside the bacteria. Some, but not all, bacteria will retain these purple complexes throughout the rest of the Gram stain procedure. Gram's iodine is also called the "mordant."
The last picture shows how the bacteria would look if the Gram stain procedure were stopped at this step.
The last picture shows how the bacteria would look if the Gram stain procedure were stopped at this step.
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