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Annabelle Elsner, Eckerd College, Biology Discipline
Yang Yang, Institute for Fundamental Biomedical Research, Johns Hopkins All Children’s Hospital
Anant Jaiswal, Institute for Fundamental Biomedical Research, Johns Hopkins All Children’s Hospital
Anne Lynch, Institute for Fundamental Biomedical Research, Johns Hopkins All Children’s Hospital
Crystal Young-Erdos, Eckerd College, Chemistry and Biochemistry Discipline
Timothy Osborne, Institute for Fundamental Biomedical Research, Johns Hopkins All Children’s Hospital
Gene expression control is vital to regulate cellular activity and responses. Histone methylation alters the chemical interactions between histone complexes, DNA, and other proteins and is one way that gene expression is controlled. SETDB2 is a H3K9 methyltransferase that is highly associated with the proinflammatory response and plays a key role in liver metabolism. The Osborne lab investigates the mechanism of SETDB2 as a regulator of gene expression for metabolic and immune systems. SETDB2 is both promoted under metabolic stressors and associated with metabolic and inflammatory diseases. Therefore, we hypothesize that SETDB2 deficiency will have protective effects against immuno-metabolic disease phenotypes. Detection of SETDB2 via immunoblot is essential to confirm the cell specific knockout of SETDB2 in mice. The goal of this project is to optimize immunoblot conditions for SETDB2 detection in mouse hepatocytes and bone marrow macrophages.
Validation Of SETDB2 Protein Expression In Setdb2 Cell-specific KnockoutsAE7.pdfFor more information: agelsner@eckerd.edu
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