Works Cited

Summary

    I wish to thank Dr. Kevin Pegg of Florida State College at Jacksonville for training me in the skills of biotechnology and allowing me to use the FSCJ laboratory for my independent cell culture research.  I also wish to thank Dr. Kathryn Kehoe of Ponte Vedra High School for being my biotechnology teacher and allowing me to use the PVHS laboratory for my plasmid preparation work.  I am thankful for all of the scientists who granted me interviews while I learned more about my area of research.  Finally, I wish to thank Dr. Jonathan Finn for providing me with the R023 plasmid containing the autogene system he has developed, as I hope to make use of this system in developing the exosomal vector.

Personal Communication

Dr. Joseph Butterfield. Email via secretary Sally Axtell. Mar. 2010.

Dr. William Anderson. Personal interview. 23 Jun. 2010. While attending Harvard Summer School.

Dr. Yitzhak Pilpel. Personal interview. 1 Jul. 2010. While attending Harvard Summer School.

Dr. Jack Szostak. (NOBEL LAUREATE) Personal interview. 5 Jul. 2010. While attending Harvard Summer School.

Dr. Johan Skog and Dr. Xandra Breakefield. Personal interview. 23 Jul. 2010. While attending Harvard Summer School.

Dr. Kevin Pegg. Personal interviews. 31 Aug. 2010, 20 Oct. 2010.

Dr. Ambili Nair. Email. 20 Sep. 2010.

Dr. Chris Ullman. Email. 23 Sep. 2010.

Dr. Kathryn Kehoe. Personal interviews. Sep. – Oct. 2010.

Dr. Jonathan Finn. Email. Oct. 2010.

Books

Campbell N., Reece J., et al. Biology. 8th ed. San Francisco: Pearson Benjamin Cummings, 2008.

Nicholl D. An Introduction to Genetic Engineering. 2nd ed. Cambridge: Cambridge University Press, 2002.

Nicholl D. An Introduction to Genetic Engineering. 3rd ed. Hong Kong: Cambridge University Press, 2008.

Park S., Cochran J. Protein Engineering and Design. 1st ed. Boca Raton: CRC Press, 2010.

Sadava D. et al. Life: The Science of Biology. 9th ed. Gordonsville: W. H. Freeman & Co., 2009.

Research Papers

Alvisi G. et al. Regulated nucleocytoplasmic targeting of viral gene products: A therapeutic target? Biochimica et Biophysica Acta. 1784, 213-227. (2008).

Belting M., Wittrup A. Nanotubes, exosomes, and nucleic acid-binding peptides provide novel mechanisms of intercellular communication in eukaryotic cells: implications in health and disease. The Journal of Cell Biology. 183, 7. (2008).

Chowdhury E. H. Nuclear targeting of viral and non-viral DNA. Expert Opinion Drug Delivery. 6, 7. (2009).

Dean, D.A. et al. Nuclear entry of nonviral vectors. Gene Therapy. 12, 881-890. (2005).

Delcayre A. et al. Exosome Display technology: Applications to the development of new diagnostics and therapeutics. Blood Cells, Molecules, and Diseases. 35, 158-168. (2005).

Finn, J.D. et al. An enhanced autogene-based dual-promoter cytoplasmic expression system yields increased gene expression. Nature Gene Therapy. 11, 276-283 (2004).

Finn J.D. et al. Investigation of factors responsible for cell line cytoplasmic expression differences. BMC Molecular Biology. 6, 11. (2005).

Finn J.D. et al. Factors limiting autogene-based cytoplasmic expression systems. The FASEB Journal. Express article 10.1096/fj.04-2769fje. (2005).

Gassart, A. et al. Lipid raft-associated protein sorting in exosomes. Hematopoiesis. 102, 13. (2003).

Gould, S., Booth, A., Hildreth J. The Trojan exosome hypothesis. Proceedings of the National Academy of Sciences of the United States of America. 100, 10592-10597 (2003).

Guescini M. et al. C2C12 myoblasts release micro-vesicles containing mtDNA and proteins involved in signal transduction. Experimental Cell Research. 316, 1977-1984. (2010).

Guescini M. et al. Astrocytes and Glioblastoma cells release exosomes carrying mtDNA. Journal of Neural Transmission. 117, 1-4. (2010).

Jackson, D.A., et al. Designing Nonviral Vectors for Efficient Gene Transfer and Long-Term Gene Expression. Molecular Therapy. 14, 5. (2006).

Lam A.P., Dean D.A. Progress and Prospects: nuclear import of nonviral vectors. Gene Therapy. 17, 439-447. (2010).

Lotvall J., Valadi H. Cell to Cell Signaling via Exosomes Through esRNA. Cell Adhesion and Migration. 1, 3. (2007).

Lufino M., et al. Advances in High-capacity Extrachromosomal Vector Technology: Episomal Maintenance, Vector Delivery, and Transgene Expression. Molecular Therapy. 16, 9. (2008).

Miller A.M., Dean D.A. Tissue-specific and transcription factor-mediated nuclear entry of DNA. Advanced Drug Delivery Reviews. 61, 603-613. (2009).

Mori Y., Koike M., et al. Human Herpesvirus-6 Induced MVB Formation, and Virus Egress Occurs by an Exosomal Release Pathway. Traffic. 9, 1728-1742. (2008).

Munkonge F. et al. Identification and Functional Characterization of Cytoplasmic Determinants of Plasmid DNA Nuclear Import. The Journal of Biological Chemistry. 284, 39. (2009).

Mutsafi Y. et al. Vaccinia-like cytoplasmic replication of the giant Mimivirus. Proceedings of the National Academy of Sciences of the United States of America. 107, 5978-5982. (2010).

Okayama Y., Kawakami T. Development, Migration, and Survival of Mast Cells. Immunologic Research. 34, 97-115. (2006).

Odegrip R. et al. CIS Display: In vitro selection of peptides from libraries of protein-DNA complexes. Proceedings of the National Academy of Sciences. 101, 2806-2810. (2004).

Pegtel D. et al. Functional delivery of viral miRNAs via exosomes. Proceedings of the National Academy of Sciences of the United States of America. 107, 14. (2010).

Pouton C. et al. Targeted delivery to the nucleus. Advanced Drug Delivery Reviews. 59, 698-717. (2007).

Radinger M. et al. Generation, Isolation, and Maintenance of Human Mast Cells and Mast Cell Lines. Current Protocols in Immunology, author manuscript released in PMC 2010 August 19. (PMC2923748)

Schubert M., et al. Gene Delivery to the Nervous System. Molecular Therapy. 16, 4. (2008).

Seoq Y., Wood M. Biological gene delivery vehicles: beyond viral vectors. Molecular Therapy. 17, 767-777 (2009).

Simons M., Raposo G. Exosomes – vesicular carriers for intercellular communication. Current Opinion in Cell Biology. 21, 575-581. (2009).

Simpson R. J. et al. Exosomes: proteomic insights and diagnostic potential. Expert Reviews Proteomics. 6, 267-283 (2009).

Skinner et al. Cellular Microvesicle Pathways Can Be Targeted to Transfer Genetic Information between Non-Immune Cells. PLoS ONE. 4, e6219.

Skog, J., et al. Glioblastoma microvesicles transport RNA and proteins that promote tumor growth and provide diagnostic biomarkers. Nature Cell Biology. 10, 12. (2008).

Smalheiser N. R. Exosomal transfer of proteins and RNAs at synapses in the nervous system. Biology Direct. 2, 35. (2007).

Stoorvogel W. The Biogenesis and Functions of Exosomes. Traffic. 3, 321-330. (2002).

Trajkovic et al. Ceramide Triggers Budding of Exosome Vesicles into Multivesicular Endosomes. Science. 319, 1244-1247. (2008).

Valadi H. et al. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nature Cell Biology. 9, 654-659 (2007).

Vaysse L et al. Development of a Self-Assembling Nuclear Targeting Vector System Based on the Tetracycline Repressor Protein. The Journal of Biological Chemistry. 279, 555-5564. (2003).

Wagstaff K., Jans D. Nucleocytoplasmic transport of DNA: enhancing non-viral gene transfer. Biochemical Journal. 406, 185-202. (2007).

Wang et al. The Carboxy-Terminal Domain of Atypical Protein Kinase C[zeta] Binds to Ceramide and Regulates Junction Formation in Epithelial Cells. Journal of Biological Chemistry. 284, 14469-14475. (2009).

Yanagida M. et al. Effects of T-Helper 2-Type Cytokines, Interleukin-3 (IL-3), IL-4, IL-5, and IL-6 on the Survival of Cultured Human Mast Cells. Blood. 86, 10. (1995).

Zhang F. et al. A Novel Strategy for the Invasive Toxin: Hijacking Exosome-Mediated Intercellular Trafficking. Traffic. 10, 411-424. (2009).

Zhi-Qing H. et al. Regulation of Mast Cell Development by Inflammatory Factors. Current Medical Chemistry. 14, 3044-3050. (2007).

Website

National Center for Biotechnology Information. National Library of Medicine, National Institutes of Health. Web. Summer 2010. <http://www.ncbi.nlm.nih.gov/>.