Real Time PCR, Denaturing gradient gel electrophoresis, Terminal fragment length polymorphism, Polymerase chain reaction, Cloning, DNA, RNA isolation from soil, Bacterial identification and characterization, Genomic & plasmid DNA isolation from Prokaryotes, Gene Cloning and sequencing in prokaryotic system, PCR, Gel Electrophoresis, Restriction digestion of plasmid DNA, Microbiology techniques, Gas Chromatography, HPLC, Thin Layer Chromatography (TLC) SDS-PAGE, ABI Sequence scanner, NCBI, Vec screen, Clustal W, Clustal X, Bioedit, Phylip 3.69, Tree view, Primer 3, Eurofins MWG Operon primer design, Sequence analyzer software for assemble the nucleotide sequence, Clone Manager for sequence analysis Sigmaplot, Sigmastats, Prism, Past3 and MVSP.