Having been constructed in the 16th Century, microscopes have revolutionized science with their ability to magnify small objects such as microbial cells, producing images with definitive structures that are identifiable and characterizable.
Derived from Greek words “mikrós” meaning “small” and “skópéō” meaning “look at”.
Microscopes are instruments that are used in science laboratories to visualize very minute objects, such as cells and microorganisms, giving a contrasting image that is magnified.
Microscopes are made up of lenses for magnification, each with its own magnification powers. Depending on the type of lens, it will magnify the specimen according to its focal strength.
Their ability to function is because they have been constructed with special components that enable them to achieve high magnification levels. They can view very small specimens and distinguish their structural differences, for example, the view of animal and plant cells viewing microscopic bacterial cells.
There are different types of microscopes like light microscope, dark-field microscope, phase contrast microscope, electron microscope, fluorescent microscope, etc.
Microscopes are generally made up of structural parts for holding and supporting the microscope and its components and the optical parts that are used for magnification and viewing of the specimen images. Modern microscopes have additional electronics and display devices. This description defines the parts of a microscope and the functions they perform to enable the visualization of specimens.
There are three structural parts of the microscope i.e. head, arm, and base.
The head is a cylindrical metallic tube that holds the eyepiece lens at one end and connects to the nose piece at other end. It is also called a body tube or eyepiece tube. It connects the eyepiece lens to the objective lens. The light coming from objectives will bend inside this tube. In binocular microscopes, they are adjustable so that the viewer can adjust the eyepiece for maximum visualization.
This is the part connecting the base to the head and the eyepiece tube to the base of the microscope. It supports the head of the microscope and is also used when carrying the microscope. Some high-quality microscopes have an articulated arm with more than one joint, allowing more movement of the microscopic head for better viewing.
The base is the lowermost part of the microscope that supports the entire microscope structure. It provides stability for the microscope. Illuminators, light switches, and electrical wiring systems are fitted in the base.
The optical parts of the microscope are used to view, magnify, and produce an image from a specimen placed on a slide. These parts include:
Eyepiece: Where you look; magnifies the image again.
Eyepiece Tube: Holds the eyepiece.
Diopter Adjustment: Focuses one eyepiece for vision differences (binocular only).
Nosepiece: Rotates to change objective lenses.
Objective Lenses: Magnify the specimen (4x, 10x, 40x, 100x).
Fine Adjustment Knob: Sharpens the image (small movements).
Coarse Adjustment Knob: Moves the stage for initial focus (large movements).
Stage: Platform for holding the specimen slide.
Stage Control Knobs: Move the slide on the stage.
Aperture: Hole in the stage for light.
Microscopic Illuminator: Light source.
Condenser: Focuses light onto the specimen.
Diaphragm: Controls the amount of light.
Condenser Focus Knob: Adjusts condenser height/focus.
Abbe Condenser: Advanced condenser for high magnification.
Rack Stop: Prevents damage by limiting stage movement.
Light Switch: Turns the light on/off.
Brightness Adjustment: Controls light intensity.