2024:

Ellenburg, W. H., & Oprian, D. D. (2024). Understanding mechanisms of terpene synthases using substrate analogs. Methods in Enzymology. https://doi.org/10.1016/bs.mie.2024.04.003

Ramasamy P. Kumar, Jason O. Matos, Brandon Y. Black, William H. Ellenburg, Jiahua Chen, MacKenzie Patterson, Jacob A. Gehtman, Douglas L. Theobald, Isaac J. Krauss, Daniel D. Oprian (2024) Crystal Structure of Caryolan-1-ol Synthase, a Sesquiterpene Synthase Catalyzing an Initial Anti-Markovnikov Cyclization Reaction. bioRxiv 2024.05.04.592530; doi: https://doi.org/10.1101/2024.05.04.592530


2023:

Schiff, W. H., & Oprian, D. D. (2023). Mutational analysis of (+)-limonene synthase. Biochemistry. https://doi.org/10.1021/acs.biochem.3c00217


2020:

Matos, J. O., Kumar, R. P., Ma, A. C., Patterson, M., Krauss, I. J., & Oprian, D. D. (2020). Mechanism underlying anti-markovnikov addition in the reaction of pentalenene synthase. Biochemistry, 59(35), 3271-3283.

2019:

Morehouse, B. R., Kumar, R. P., Matos, J. O., Yu, Q., Bannister, A., Malik, K., ... & Oprian, D. D. (2019). Direct evidence of an enzyme-generated LPP intermediate in (+)-limonene synthase using a fluorinated GPP substrate analog. ACS chemical biology, 14(9), 2035-2043.

Ozdeslik, R. N., Olinski, L. E., Trieu, M. M., Oprian, D. D., & Oancea, E. (2019). Human nonvisual opsin 3 regulates pigmentation of epidermal melanocytes through functional interaction with melanocortin 1 receptor. Proceedings of the National Academy of Sciences, 116(23), 11508-11517.


2017: 

Lamarche, L. B., Kumar, R. P., Trieu, M. M., Devine, E. L., Cohen-Abeles, L. E., Theobald, D. L., & Oprian, D. D. (2017). Purification and characterization of RhoPDE, a retinylidene/phosphodiesterase fusion protein and potential optogenetic tool from the choanoflagellate Salpingoeca rosetta. Biochemistry, 56(43), 5812-5822.

Kumar, R. P., Morehouse, B. R., Matos, J. O., Malik, K., Lin, H., Krauss, I. J., & Oprian, D. D. (2017). Structural characterization of early Michaelis complexes in the reaction catalyzed by (+)-limonene synthase from Citrus sinensis using fluorinated substrate analogues. Biochemistry, 56(12), 1716-1725.

Morehouse, B. R., Kumar, R. P., Matos, J. O., Olsen, S. N., Entova, S., & Oprian, D. D. (2017). Functional and structural characterization of a (+)-limonene synthase from Citrus sinensis. Biochemistry, 56(12), 1706-1715.

2016:

Chakrabarti, K. S., Agafonov, R. V., Pontiggia, F., Otten, R., Higgins, M. K., Schertler, G. F., ... & Kern, D. (2016). Conformational selection in a protein-protein interaction revealed by dynamic pathway analysis. Cell reports, 14(1), 32-42.

Devine, E. L., Theobald, D. L., & Oprian, D. D. (2016). Relocating the active-site lysine in rhodopsin: 2. Evolutionary intermediates. Biochemistry, 55(34), 4864-4870.

2015:

Kumar, R. P., Ranaghan, M. J., Ganjei, A. Y., & Oprian, D. D. (2015). Crystal structure of recoverin with calcium ions bound to both functional EF hands. Biochemistry, 54(49), 7222-7228.


2014:

D’Antona, A. M., Xie, G., Sligar, S. G., & Oprian, D. D. (2014). Assembly of an activated rhodopsin–transducin complex in nanoscale lipid bilayers. Biochemistry, 53(1), 127-134.

2013:

Devine, E. L., Oprian, D. D., & Theobald, D. L. (2013). Relocating the active-site lysine in rhodopsin and implications for evolution of retinylidene proteins. Proceedings of the National Academy of Sciences, 110(33), 13351-13355.

Ranaghan, M. J., Kumar, R. P., Chakrabarti, K. S., Buosi, V., Kern, D., & Oprian, D. D. (2013). A highly conserved cysteine of neuronal calcium-sensing proteins controls cooperative binding of Ca2+ to recoverin. Journal of Biological Chemistry, 288(50), 36160-36167.

2012: 

Deupi, X., Edwards, P., Singhal, A., Nickle, B., Oprian, D., Schertler, G., & Standfuss, J. (2012). Stabilized G protein binding site in the structure of constitutively active metarhodopsin-II. Proceedings of the National Academy of Sciences, 109(1), 119-124.

Frederiksen, Rikard, Nicholas P. Boyer, Benjamin Nickle, Kalyan S. Chakrabarti, Yiannis Koutalos, Rosalie K. Crouch, Daniel Oprian, and M. Carter Cornwall. "Low aqueous solubility of 11-cis-retinal limits the rate of pigment formation and dark adaptation in salamander rods." Journal of General Physiology 139, no. 6 (2012): 493-505.

2011:

Standfuss, J., Edwards, P. C., D’Antona, A., Fransen, M., Xie, G., Oprian, D. D., & Schertler, G. F. (2011). The structural basis of agonist-induced activation in constitutively active rhodopsin. Nature, 471(7340), 656-660.

Xie, G., D’Antona, A. M., Edwards, P. C., Fransen, M., Standfuss, J., Schertler, G. F., & Oprian, D. D. (2011). Preparation of an activated rhodopsin/transducin complex using a constitutively active mutant of rhodopsin. Biochemistry, 50(47), 10399-10407.

2007:

Standfuss, J., Xie, G., Edwards, P. C., Burghammer, M., Oprian, D. D., & Schertler, G. F. (2007). Crystal structure of a thermally stable rhodopsin mutant. Journal of molecular biology, 372(5), 1179-1188.

Bayburt, T. H., Leitz, A. J., Xie, G., Oprian, D. D., & Sligar, S. G. (2007). Transducin activation by nanoscale lipid bilayers containing one and two rhodopsins. Journal of biological chemistry, 282(20), 14875-14881.

2006:

Chen, J., Shi, G., Concepcion, F. A., Xie, G., Oprian, D., & Chen, J. (2006). Stable rhodopsin/arrestin complex leads to retinal degeneration in a transgenic mouse model of autosomal dominant retinitis pigmentosa. Journal of Neuroscience, 26(46), 11929-11937.

Higgins, M. K., Oprian, D. D., & Schertler, G. F. (2006). Recoverin binds exclusively to an amphipathic peptide at the N terminus of rhodopsin kinase, inhibiting rhodopsin phosphorylation without affecting catalytic activity of the kinase. Journal of Biological Chemistry, 281(28), 19426-19432.

Tam, B. M., Xie, G., Oprian, D. D., & Moritz, O. L. (2006). Mislocalized rhodopsin does not require activation to cause retinal degeneration and neurite outgrowth in Xenopus laevis. Journal of Neuroscience, 26(1), 203-209.

2005:

Kono, M., Crouch, R. K., & Oprian, D. D. (2005). A dark and constitutively active mutant of the tiger salamander UV pigment. Biochemistry, 44(2), 799-804.

2004:

Kim, J. M., Altenbach, C., Kono, M., Oprian, D. D., Hubbell, W. L., & Khorana, H. G. (2004). Structural origins of constitutive activation in rhodopsin: Role of the K296/E113 salt bridge. Proceedings of the National Academy of Sciences, 101(34), 12508-12513.

Das, J., Crouch, R. K., Ma, J. X., Oprian, D. D., & Kono, M. (2004). Role of the 9-methyl group of retinal in cone visual pigments. Biochemistry, 43(18), 5532-5538.

2003:

Jin, S., Cornwall, M. C., & Oprian, D. D. (2003). Opsin activation as a cause of congenital night blindness. Nature neuroscience, 6(7), 731-735.

Jin, S., McKee, T. D., & Oprian, D. D. (2003). An improved rhodopsin/EGFP fusion protein for use in the generation of transgenic Xenopus laevis. FEBS letters, 542(1-3), 142-146.

Oprian, D. D. (2003). Phototaxis, chemotaxis and the missing link. Trends in biochemical sciences, 28(4), 167-169.

Gross, A. K., Rao, V. R., & Oprian, D. D. (2003). Characterization of rhodopsin congenital night blindness mutant T94I. Biochemistry, 42(7), 2009-2015.

Gross, A. K., Xie, G., & Oprian, D. D. (2003). Slow binding of retinal to rhodopsin mutants G90D and T94D. Biochemistry, 42(7), 2002-2008.

Xie, G., Gross, A. K., & Oprian, D. D. (2003). An opsin mutant with increased thermal stability. Biochemistry, 42(7), 1995-2001.

2002:

Fasick, J. I., Applebury, M. L., & Oprian, D. D. (2002). Spectral tuning in the mammalian short-wavelength sensitive cone pigments. Biochemistry, 41(21), 6860-6865.

Abdulaev, N. G., Strassmaier, T. T., Ngo, T., Chen, R., Luecke, H., Oprian, D. D., & Ridge, K. D. (2002). Grafting segments from the extracellular surface of CCR5 onto a bacteriorhodopsin transmembrane scaffold confers HIV-1 coreceptor activity. Structure, 10(4), 515-525.


2001:

Ma, J. X., Znoiko, S., Othersen, K. L., Ryan, J. C., Das, J., Isayama, T., ... & Crouch, R. K. (2001). A visual pigment expressed in both rod and cone photoreceptors. Neuron, 32(3), 451-461.

MA, J. X., KONO, M., XU, L., DAS, J., RYAN, J. C., HAZARD, E. S., ... & CROUCH, R. K. (2001). Salamander UV cone pigment: sequence, expression, and spectral properties. Visual neuroscience, 18(3), 393-399.

2000:

Struthers, M., Yu, H., & Oprian, D. D. (2000). G protein-coupled receptor activation: analysis of a highly constrained,“straitjacketed” rhodopsin. Biochemistry, 39(27), 7938-7942.

Zhou, G., Ferrer, M., Chopra, R., Kapoor, T. M., Strassmaier, T., Weissenhorn, W., ... & Wiley, D. C. (2000). The structure of an HIV-1 specific cell entry inhibitor in complex with the HIV-1 gp41 trimeric core. Bioorganic & medicinal chemistry, 8(9), 2219-2227.

Struthers, M., & Oprian, D. D. (2000). Mapping tertiary contacts between amino acid residues within rhodopsin. Methods in enzymology, 315, 130-143.

1999:

Fasick, J. I., Lee, N., & Oprian, D. D. (1999). Spectral tuning in the human blue cone pigment. Biochemistry, 38(36), 11593-11596.

Ferrer, M., Kapoor, T. M., Strassmaier, T., Weissenhorn, W., Skehel, J. J., Oprian, D., ... & Harrison, S. C. (1999). Selection of gp41-mediated HIV-1 cell entry inhibitors from biased combinatorial libraries of non-natural binding elements. Nature structural biology, 6(10), 953-960.

Yu, H., Kono, M., & Oprian, D. D. (1999). State-dependent disulfide cross-linking in rhodopsin. Biochemistry, 38(37), 12028-12032.

Struthers, M., Yu, H., Kono, M., & Oprian, D. D. (1999). Tertiary interactions between the fifth and sixth transmembrane segments of rhodopsin. Biochemistry, 38(20), 6597-6603.

Yu, H., & Oprian, D. D. (1999). Tertiary interactions between transmembrane segments 3 and 5 near the cytoplasmic side of rhodopsin. Biochemistry, 38(37), 12033-12040.

1998:

 Kono, M., Yu, H., & Oprian, D. D. (1998). Disulfide bond exchange in rhodopsin. Biochemistry, 37(5), 1302-1305.

1997:

 Kochendoerfer, G. G., Wang, Z., Oprian, D. D., & Mathies, R. A. (1997). Resonance Raman examination of the wavelength regulation mechanism in human visual pigments. Biochemistry, 36(22), 6577-6587.

Yang, T., Snider, B. B., & Oprian, D. D. (1997). Synthesis and characterization of a novel retinylamine analog inhibitor of constitutively active rhodopsin mutants found in patients with autosomal dominant retinitis pigmentosa. Proceedings of the National Academy of Sciences, 94(25), 13559-13564.

Rim, J., Faurobert, E., Hurley, J. B., & Oprian, D. D. (1997). In vitro assay for trans-phosphorylation of rhodopsin by rhodopsin kinase. Biochemistry, 36(23), 7064-7070.

1996:

 Rao, V. R., & Oprian, D. D. (1996). Activating mutations of rhodopsin and other G protein-coupled receptors. Annual review of biophysics and biomolecular structure, 25(1), 287-314.


1995:

 Rim, J., & Oprian, D. D. (1995). Constitutive activation of opsin: interaction of mutants with rhodopsin kinase and arrestin. Biochemistry, 34(37), 11938-11945.

Yu, H., Kono, M., McKee, T. D., & Oprian, D. D. (1995). A general method for mapping tertiary contacts between amino acid residues in membrane-embedded proteins. Biochemistry, 34(46), 14963-14969.

Oprian, D. D. (1995). Structure of the cGMP-gated channel. Behavioral and Brain Sciences, 18(3), 482-483.


1994:

 Asenjo, A. B., Rim, J., & Oprian, D. D. (1994). Molecular determinants of human red/green color discrimination. Neuron, 12(5), 1131-1138.

Rao, V. R., Cohen, G. B., & Oprian, D. D. (1994). Rhodopsin mutation G90D and a molecular mechanism for congenital night blindness. Nature, 367(6464), 639-642.


1993:

 Dryja, T. P., Berson, E. L., Rao, V. R., & Oprian, D. D. (1993). Heterozygous missense mutation in the rhodopsin gene as a cause of congenital stationary night blindness. Nature genetics, 4(3), 280-283.

Cohen, G. B., Yang, T., Robinson, P. R., & Oprian, D. D. (1993). Constitutive activation of opsin: influence of charge at position 134 and size at position 296. Biochemistry, 32(23), 6111-6115.

Wang, Z., Asenjo, A. B., & Oprian, D. D. (1993). Identification of the chloride-binding site in the human red and green color vision pigments. Biochemistry, 32(9), 2125-2130.

Oprian, D. D. (1993). Expression of opsin genes in COS cells. In Methods in Neurosciences (Vol. 15, pp. 301-306). Academic Press.


1992:

     Robinson, P. R., Cohen, G. B., Zhukovsky, E. A., & Oprian, D. D. (1992). Constitutively active mutants of rhodopsin. Neuron, 9(4), 719-725.

Cohen, G. B., Oprian, D. D., & Robinson, P. R. (1992). Mechanism of activation and inactivation of opsin: role of Glu113 and Lys296.          Biochemistry, 31(50), 12592-12601.

Oprian, D. D. (1992). The ligand-binding domain of rhodopsin and other G protein-linked receptors. Journal of bioenergetics and biomembranes, 24, 211-217.

Zhukovsky, E. A., Robinson, P. R., & Oprian, D. D. (1992). Changing the location of the Schiff base counterion in rhodopsin. Biochemistry, 31(42), 10400-10405.

Zhukovsky, E. A., Robinson, P. R., & Oprian, D. D. (1992). Changing the location of the Schiff base counterion in rhodopsin. Biochemistry, 31(42), 10400-10405.

Oprian, D. D. (1992). Molecular determinants of spectral properties and signal transduction in the visual pigments. Current Opinion in Neurobiology, 2(4), 428-432.

1991:

Oprian, D. D., Asenjo, A. B., Lee, N., & Pelletier, S. L. (1991). Design, chemical synthesis, and expression of genes for the three human color vision pigments. Biochemistry, 30(48), 11367-11372.

Zhukovsky, E. A., Robinson, P. R., & Oprian, D. D. (1991). Transducin activation by rhodopsin without a covalent bond to the 11-cis-retinal chromophore. Science, 251(4993), 558-560.


1989:

Zhukovsky, E. A., & Oprian, D. D. (1989). Effect of carboxylic acid side chains on the absorption maximum of visual pigments. Science, 246(4932), 928–930. https://doi.org/10.1126/science.2573154