Recent Publications
Zhang X, Van Treeck B, Horton CA, McIntyre JJR, Palm SM, Shumate JL, Collins K. Harnessing eukaryotic retroelement proteins for transgene insertion into human safe-harbor loci. Nature Biotech (2024).
Thawani A, Florez Ariza AJ, Nogales E, Collins K. Template and target site recognition by human LINE-1 in retrotransposition. Nature 626: 186-193 (2024).
Ferguson L, Upton HE, Pimentel SC, Mok A, Lareau LF, Collins K, Ingolia NT. Streamlined and sensitive mono- and di-ribosome profiling in yeast and human cells. Nature Methods 20: 1704-1715 (2023).
Shaw A, Craig JM, Amiri H, Kim J, Upton HE, Pimentel SC, Huang JR, Marqusee S, Collins K, Gundlach JH, Bustamante CJ. Nanopore molecular trajectories of a eukaryotic reverse transcriptase reveal a long-range RNA structure sensing mechanism. bioRxiv 1:2023.04.05.535757 (2023).
Gustafsson TH, Galan C, Yu T, Upton HE, Ferguson F, Kaymak E, Weng Z, Collins K, Rando OJ. Deep sequencing of yeast and mouse tRNAs and tRNA fragments using OTTR. bioRxiv doi: https://doi.org/10.1101/2022.02.04.479139 (2022).
Pimentel SC, Upton HE, Collins K. Separable structural requirements for cDNA synthesis, nontemplated extension, and template jumping by a non-LTR retroelement reverse transcriptase. J Biol Chem 298: 101624 (2022).
Upton HE, Ferguson L, Temoche-Diaz MM, Liu XM, Pimentel SC, Ingolia NT, Schekman R, Collins K. Low-bias ncRNA libraries using ordered two-template relay: Serial template jumping by a modified retroelement reverse transcriptase. PNAS 118: e2107900118 (2021).
Ghanim GE, Fountain AJ, van Roon AM, Rangan R, Das R, Collins K, Nguyen THD. Structure of human telomerase holoenzyme with bound telomeric DNA. Nature 593:449-453 (2021).
Boyle JM, Hennick KM, Regalado SG, Vogan JM, Zhang X, Collins K, Hockemeyer D. Telomere length set point regulation in human pluripotent stem cells critically depends on the shelterin protein TPP1. Mol Biol Cell 31: 2583-2596 (2020).
Nguyen THD, Collins K, Nogales E. Telomerase structures and regulation: shedding light on the chromosome end. Curr Opin Struct Biol 55: 185-193 (2019).
Deshpande AP, Collins K. Mechanisms of template handling and pseudoknot folding in human telomerase and their manipulation to expand the sequence repertoire of processive repeat synthesis. Nucleic Acids Res 2018 46:7886-7901 (2018).
Nguyen THD, Tam J, Wu RA, Greber BJ, Toso D, Nogales E, Collins K. Cryo-EM structure of substrate-bound human telomerase holoenzyme. Nature 557: 190-5 (2018).
Wu RA, Upton HE, Vogan JM, Collins K. Telomerase mechanisms of telomere synthesis. Ann Rev Biochem 86: 439-60 (2017).
Wu RA, Tam J, Collins K. DNA-binding determinants and cellular thresholds for human telomerase repeat addition processivity. EMBO J 36: 1908-27 (2017).
Chiba K, Vogan JM, Wu RA, Gill MS, Zhang X, Collins K, Hockemeyer H. Endogenous TERT N-terminal tagging affects human telomerase function at telomeres in vivo. MCB 37: e00541-16 (2017).
Farley BM, Collins K. Trans-generational function of Tetrahymena Piwi protein Twi8p at distinctive non-coding RNA loci. RNA 23: 530-45 (2017).
Upton HE, Chan H, Feigon J, Collins K. Replication Protein A subunits assemble alternative complexes for general single-stranded DNA binding or telomeric-repeat DNA binding by Tetrahymena telomerase holoenzyme. J Biol Chem 292: 217-28 (2017).
Vogan JM, Zhang X, Youmans DT, Regalado SG, Johnson JZ, Hockemeyer D, Collins K. Minimized human telomerase maintains telomeres and resolves endogenous roles of H/ACA proteins, TCAB1, and Cajal bodies. eLife 5: e18221 (2016).
Wu RA, Dagdas YS, Yilmaz ST, Yildiz A, Collins K. Single-molecule imaging of telomerase reverse transcriptase in human telomerase holoenzyme and minimal RNP complexes. eLife 4: e08363 1-26 (2015).
Jiang J, Chan H, Cash DD, Miracco EJ, Loo RRO, Upton HE, Cascio D, Johnson RO, Collins K, Loo JA, Zhou ZH, Feigon J. Structure of Tetrahymena telomerase reveals previously unknown subunits, functions, and interactions. Science 350: aab4070 1-10 (2015).
Wan B, Tang T, Upton H, Shuai J, Zhou Y, Li S, Chen J, Brunzelle JS, Zeng Z, Collins K, Wu J, Lei M. The Tetrahymena telomerase p75-p45-p19 subcomplex is a unique CST complex. NSMB 22: 1023-6 (2015).
Hockemeyer D, Collins K. Control of telomerase action at human telomeres. NSMB 2015 Nov;22: 848-52 (2015).
Vogan JM, Collins K. Dynamics of human telomerase holoenzyme assembly and subunit exchange across the cell cycle. J Biol Chem 290: 21320-35 (2015).
Wu RA, Collins K. Sequence specificity of human telomerase. PNAS 111: 11234-5 (2014). PDF
Upton HE, Hong K, Collins K. Direct single-stranded DNA binding by Teb1 mediates the recruitment of Tetrahymena telomerase to telomeres. MCB 34: 4200-12 (2014). PDF
Sexton AN, Regalado SG, Lai CS, Cost GJ, O'Neil CM, Urnov FD, Gregory PD, Jaenisch R, Collins K, Hockemeyer D. Genetic and molecular identification of three human TPP1 functions in telomerase action: Recruitment, activation, and homeostasis set-point regulation. Genes Dev 28: 1885-99 (2014). PDF
Katibah GE, Qin Y, Sidote DJ, Yao J, Lambowitz AM, Collins K. Broad and adaptable RNA structure recognition by the human interferon-induced tetratricopeptide repeat protein IFIT5. PNAS 111: 12025-30 (2014). PDF
Wu RA, Collins K. Human telomerase specialization for repeat synthesis by unique handling of primer-template duplex. EMBO J. 33: 921-35 (2014). PDF
Hong K, Upton H, Miracco EJ, Jiang J,Zhou ZH, Feigon J, Collins K. Tetrahymena telomerase holoenzyme assembly, activation, and inhibition by domains of the p50 central hub. MCB 33: 3962-71 (2013). PDF
Jiang J, Miracco EJ, Hong K, Eckert B, Chan H, Cash DD, Min B, Zhou ZH, Collins K, Feigon J. The architecture of Tetrahymena telomerase holoenzyme.Nature 496: 187-92 (2013). PDF
Katibah GE, Lee HJ, Huizar JP, Vogan JM, Alber T, Collins K. tRNA binding, structure, and localization of the human interferon-induced protein IFIT5. Mol Cell49: 743-50 (2013). PDF
Collins K, Nilsen TW. Enzyme engineering through evolution: Thermostable recombinant group II intron reverse transcriptases provide new tools for RNA research and biotechnology. RNA 1017-8 (2013). PDF
Egan ED, Collins K. Biogenesis of telomerase ribonucleoproteins. RNA 18: 1747-59 (2012). PDF
Couvillion MT, Bounova G, Purdom E, Speed TP, Collins K. A Tetrahymena Piwi bound to mature tRNA 3' fragments activates the exonuclease Xrn2 for RNA processing in the nucleus. Mol Cell 48: 509-20 (2012). PDF
Sexton AN, Youmans DT, Collins K. Specificity requirements for human telomere protein interaction with telomerase holoenzyme. J Biol Chem 287: 4455-64 (2012). PDF
Talsky KB, Collins K. Strand-asymmetric endogenous Tetrahymena small RNA production requires a previously uncharacterized uridylyltransferase protein partner. RNA 18: 1553-62 (2012). PDF