Recent Publications 

Thawani A, Rodríguez-Vargas A, Van Treeck B,  Hassan NT, Adelson DL, Nogales E, Kathleen Collins K. Structures of vertebrate R2 retrotransposon complexes during target-primed reverse transcription and after second strand nicking. bioRXiv

Ferguson L, Upton HE, Pimentel SC, Jeans C, Ingolia NT, Collins K. Improved precision, sensitivity, and adaptability of Ordered Two-Template Relay cDNA library preparation for RNA sequencing. RNA (in press), bioRXiv

Palm SM, Van Treeck B, Collins K. Experimental considerations for precise RNA-mediated insertion of transgenes. Methods Enzymol (2024). 

Palm SM, Horton CA, Zhang X, Collins K. Structure and sequence at an RNA template 5´ end influence insertion of transgenes by an R2 retrotransposon protein. RNA (2024).

Lee RJ, Horton CA, Van Treeck B, McIntyre JJR, Collins K. Conserved and divergent DNA recognition specificities and functions of R2 retrotransposon N-terminal domains. Cell Reports (2024).

Zhang X, Van Treeck B, Horton CA, McIntyre JJR, Palm SM, Shumate JL, Collins K. Harnessing eukaryotic retroelement proteins for transgene insertion into human safe-harbor loci. Nature Biotech (2024).

Rodríguez-Vargas A, Collins K. Distinct and overlapping RNA determinants for binding and target-primed reverse transcription by Bombyx mori R2 retrotransposon protein. Nucleic Acids Research  (2024). 

Thawani A, Florez Ariza AJ, Nogales E, Collins K. Template and target site recognition by human LINE-1 in retrotransposition. Nature (2024).

Ferguson L, Upton HE, Pimentel SC, Mok A, Lareau LF, Collins K, Ingolia NT. Streamlined and sensitive mono- and di-ribosome profiling in yeast and human cells. Nature Methods (2023).

Shaw A, Craig JM, Amiri H, Kim J, Upton HE, Pimentel SC, Huang JR, Marqusee S, Collins K, Gundlach JH, Bustamante CJ. Nanopore molecular trajectories of a eukaryotic reverse transcriptase reveal a long-range RNA structure sensing mechanism. bioRxiv (2023).

Pimentel SC, Upton HE, Collins K. Separable structural requirements for cDNA synthesis, nontemplated extension, and template jumping by a non-LTR retroelement reverse transcriptase. J Biol Chem (2022).

Upton HE, Ferguson L, Temoche-Diaz MM, Liu XM, Pimentel SC, Ingolia NT, Schekman R, Collins K. Low-bias ncRNA libraries using ordered two-template relay: Serial template jumping by a modified retroelement reverse transcriptase. PNAS (2021).

Ghanim GE, Fountain AJ,  van Roon AM, Rangan R, Das R, Collins K, Nguyen THD. Structure of human telomerase holoenzyme with bound telomeric DNA. Nature 593:449-453 (2021).