Directions:
Collect fractions 8-12 using 2 mL of 1:1 ethyl acetate : acetone per fraction.
Deviations from procedure:
Fractions 8-14 were collected using 1 mL of 1:1 ethyl acetate : acetone per fraction. The video below shows the collection of fractions 7 and 8.
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A student finishes collecting fraction 7, then places test tube "8" under the column, adds solvent from a graduated cylinder labeled "1:1 ethyl acetate : acetone", and uses the large rubber bulb to drain the solvent into the test tube.