Part 2: Unknown 3 Plate 1

Repeat the spotting, developing, and visualization steps from part 1 with a new plate.

Recall that lane 1 is aspirin, lane 2 is your assigned unknown compound, and lane 3 is acetaminophen. The developing solvent is 10:10:1 toluene : ethyl acetate : acetic acid.

A photo of Unknown 3 Plate 1, visualized with the naked eye. No spots are visible.

Visualization: Naked Eye

A photo of Unknown 3 Plate 1, visualized under a UV lamp. UV-active spots have been circled with a pencil. All spots are quite UV-active. The spot in the unknown 3 lane is large and streaky.

Visualization: UV Lamp

Photo of Unknown 3 Plate 1, which has been stained with iodine. UV-active spots have been circled with a pencil. A ruler is located beside the plate.

Visualization: Iodine

Copy down the stained plate, making sure to label the compound spotted in each lane of the plate, the solvent used to develop the plate, the solvent front, and how each spot was visualized. Then, calculate Rf values for each spot.

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