Dr. Prabhakar Singh's research focuses on the morphological and molecular mechanisms underlying neurodegenerative diseases, particularly Alzheimer's disease, ischemia, and cognitive dysfunction associated with obstructive sleep apnea. His work aims to unravel the complex pathways that lead to neuronal degeneration and cognitive impairments, with the goal of identifying potential therapeutic targets.
Research Projects
Development of animal model of cholinergic degeneration and therapeutic interventions for Alzheimer’s disease (P.I., ICMR, Rs. 35 Lakh)
Neurological outcomes of obstructive sleep apnea and its cross generational cognitive effects in animal models (P.I., CSRI DST, Rs. ~85 Lakh)
Regional differences of transient focal brain ischemia in animal models (using stereotaxic injection of natural vasoconstrictor, endothelin-1) (P.I., AIIMS).
Developing a methodology to investigate cellular interaction of mucor in human tissue human tissue at ultrastructure level using electron microscopy (P.I., AIIMS).
Effect of Transplantation of lentivirus mediated genetically engineered mesenchymal stem cells into monocrotaline induced pulmonary hypertensive rats: validation by Next Generation Sequencing (P.I., with Department of Pharmacology, Rs. 20 Lakh, AIIMS)
Collaborative:
Development of a nanoparticle-based delivery system of Cisplatin for tissue-specific targeted drug delivery vehicles (AIIMS) (Co-Investigator, ICMR)
Study of the age-related changes in axonal cytoskeleton of the rat optic nerve (AIIMS) (Co-Investigator, ICMR)
Nanoparticles and their applications.
Cell biology and autophagy (JMI, Delhi and MNNIT Allahabad)
Ultrastructural studies of neuronal development and regeneration (IGIB, Delhi).
Research Infrastructure
Stereotactic instrument for rodent brain surgery
Transmission Electron Microscope
Scanning electron microscope
Confocal Microscope
Ultracentrifuge
HPLC and FPLC
Stereology (departmental facility)
Animal Behavioral Facility
Cell culture facility of EMF
Phase contrast Bright Field and Fluorescence microscopes (Nikon, Olympus, Zeiss, Leica)
Real-Time PCR (Bio-Rad)
Atomic Force Microscope for life science (Bio-AFM, Bruker)
Infrastructure Under-Development
Intermittent Hypoxia System for small animals (Expected installation 2024)
Involved with graduate and post-graduate education and various electron microscopy-related training programs. For information regarding training programs visit: https://saifaiims.com
Scanning Electron Micrograph of the surface of the lateral ventricle in a rat brain, highlighting ependymal cilia.
Confocal micrographs of GFAP-positive astrocytes in the hippocampus of control and amyloid beta-injected AD model.