Publications

Journal Papers:

1. Differentiate of Ten Pea Cultivars (Pisum sativum L.) by RAPD Markers and Seed Storage Proteins. Jordan Journal of Agricultural Sciences, Volume 11, No.1 2015.

  • ABSTRACT

The present study involved characterization of variability of random amplified polymorphic DNA (RAPD) markers and protein patterns in some Pisum sativum cultivars. Twenty RAPD primers were used to estimate the genetic diversity in ten pea cultivars. A total of 89 bands were scored, of which 53 bands (59.55%) were polymorphic and two of polymorphic band were unique bands. The average of amplified and polymorphic bands was 4.45 and 2.65, respectively. The highest polymorphic bands found at locus C10, R11 and V03 (5 bands), whereas the lowest number of polymorphic bands found at locus OPA-09, OPA13, OPA-07, P19, N7 and N20 (1 band). Distance matrix based on Jaccard coefficient ranged from 0.333 to 0.774. The highest value was found between O-P622 and O-P234, while the lowest genetic distance was observed between O-P923 and O-P888. A dendrogram revealed two major clusters: cluster I and cluster II. The results of dendrogram indicated that the groups of cluster II is most genetically distant from groups of cluster I. The SDS-PAGE patterns of seed storage proteins, showed that among 23 electrophoretic protein bands, only five bands were recorded to be polymorphic. The distance matrix values ranged from 0.00 to 0.75. At distance 23, cluster analysis sorting the cultivars into two major groups. The dendrogram constructed from both methods divided pea cultivars into two clusters. In this study, analysis using RAPD markers provide more information about genetic diversity among pea cultivars and the results of SDS-PAGE show low variation among pea cultivars.

2. Determination of Genetic Relationship among Some Varieties of Chickpea (Cicer arietinum L) in Sulaimani by RAPD and ISSR Markers. Jordan Journal of Biological Sciences, Volume 4, Number 2, June 2011 ISSN 1995-6673 Pages 77 – 86.

  • ABSTRACT

The molecular evaluation of five chickpea (Cicer arietinum L.) varieties [Rania, Chamchamal, Sangaw, FLIP98-133c (screened for their very sensibility to Ascochyta rabiei) and FLIP83-48c (screened for their resistance to Ascochyta rabiei)] at the University of Sulaimani, College of Agriculture, in 2009-2010 was conducted to assess the genetic diversity and relationship of chickpea genotypes using RAPD and ISSR markers. Five primers of RAPD and ISSR were used of which all primers gave amplification products. On average, 5.8 bands per primer were observed by RAPD and 6.6 bands per primer by ISSR markers. In RAPD, the varieties shared 55.17% polymorphic bands, whereas they shared 63.63% polymorphic bands in ISSR analysis. Cluster analysis by RAPD and ISSR markers revealed clear distinct diversity between genotypes. Rania and Chamchamal showed more similarity than others varieties according to the RAPD data. FLIP83-48c showed the highest dissimilarity comparing with the other varieties. In ISSR analysis, Chamchamal and Sangaw showed more similarity than others varieties. Rania revealed the highest dissimilarity comparing with the others of varieties. Combination of RAPD and ISSR data shared that FLIP83-48c showed the highest dissimilarity comparing with the other varieties. The results showed that ISSR and RAPD analysis for diversity can provide practical information for the management of genetic resources in chickpea breeding program.

3. Impact of Magnetic Application on the Parameters Related to Growth of Chickpea (Cicer arietinum L.). Jordan Journal of Biological Sciences-Volume 3, Number 4, December 2010Pages 175- 184 .

  • ABSTRACT

The morphological data were used in this study for the evaluation of five chickpea (Cicer arietinum L.) varieties at the University of Sulaimanyah, College of Agriculture in 2010. Seeds of different varieties of chickpea were exposed in batches to static magnetic fields (1500 gauss of magnetic force) for 30, 50 and 70 min. Then, the magnetic seed were placed between two layers of moist germination paper in petri dish. They were placed in the germination incubator at 20°C in an upright position. After 8 days, different plant growth parameters were tested such as germination percentage, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight and shoot dry weight, based on normal seedlings. The results showed that magnetic field application enhanced seed performance in terms of laboratory germination: seedling length and seedling fresh and dry weight compared to unexposed control. However, the response varied with duration of exposure. Among the various duration exposures, 50 and 70 min. exposures gave best results. A pot experiment was carried out in a greenhouse was aimed at finding the effect of a constant magnetic field on the root and shoot system, as well as on yield of spring chickpea. Seeds are grown in plastic cups for 4 months and irrigated with magnetized water which prepared by using static magnetic field. Six to fourteen plants were tagged for morphological data collection. The results showed that magnetized seeds irrigated with magnetized water have enhanced seed performance in terms of plant height, number of brancha, number of leaves, number of leaflets root and shoot fresh weight, root and shoot dry weight, the total photosynthetic pigments (chlorophyll a, b, and carotenoids) and yield in some varieties.

4.COMPARISON OF SOME GROWTH AND YIELD PERFORMANCE OF SOYBEAN VARIETIES (Glycine max L.) IJPAES,Volume-5, Issue-4, Oct-Dec-2015

  • ABSTRACT

Selection of suitable variety for an agro-climatic zone is of prime concern for soybean growers. Moreover, identification of suitable plant traits showing maximum contribution to final seeds yield is important for plant architects. Experiment was laid out in randomized complete block design (RCBD). Soybean variety LV19 showed best performance as it gave significantly higher plant height (36.48 cm), leaflet and leaf area (15.66 and 46 cm2 ), No. of branches per plant (6.66), No. of pods plant-1(79.66), biomass (83.45 g), Pods weight per plant (34.15 g), 100-seeds weight (9.3 g) and seeds yield per plant (25.66 g). Contrastingly, soybean variety LV88 was found to be inferior as it gave lowest value for most parameters. Seeds yield showed a significant positive relationship with leaflet are (R2 =0.89), leaf area (R2 =0.909), No. of branches per plant (R2 =0.64), and plant biomass (R2 =0.63), No. of pod per plant (R2 =0.73) and pods weight (R2 =0.73) where as non-significant negative relationship with plant height (R2 =0.21) and 100-seeds weight (R2 =0.44) was observed. The strongest relationship of seeds yield was seen with number of branches per plant (b=1.65) followed and the weakest with plant biomass (b=0.02). Therefore this traitshould be considered to be most important while selecting improved soybean genotypes under semi-arid conditions.

Key words: Soybean varieties, Seed yield, Plant traits, Regression.

5.RESPONSE OF SOME CHICKPEA (CICER ARIETINUM L.) VARIETIES UNDER RAINFED CONDITION. IJPAES,Volume-5, Issue-4, Oct-Dec-2015

  • ABSTRACT

A study was undertaken to evaluate the performance of three chickpea varieties (Flip-1, Flip-2 and Flip-3). Field experiment was performed at research area of Faculty of Agricultural Sciences, University of Sulaimani, during spring season of 2014. Experiment was laid out in randomized complete block design (RCBD). There was a significant genotypic difference with respect to all studied characters except number of branches/plant and dry shoot weight. Chickpea variety Flip-2 showed best performance as it gave significantly higher total weight (8.84 g), dry shoot weight (1.52 g), dry root weight (0.91g), and weight of pods. The highest seed yield (3.86 g per plant) was recorded in Flip-2. The associations of different characters have been assessed. There was significant positive correlation of plant height with fresh shoot weight (r=0.75*) and fresh root weight (r=0.79*). On the others hand, the correlation between plant height and dry root weight was negative (r=-0.81*). The result showed a significant negative correlation of total weight with fresh shoot weight (r=-0.79*) and fresh root weight (r=-0.79*). There was significant positive relationship between dry shoot weight and weight of pods (r=0.67*). The maximum correlation was observed by fresh shoot weight and fresh root weight (r=0.99**).

Key words: Chickpea varieties, Seed yield, Plant traits, Correlation, Biomass

6.Influence of Plant Spacing on the Growth and Yield of Groundnut (Arachis hypogaeaL.) IJCRBP, Volume:3 Issue-10, Oct. 2016

  • ABSTRACT

The field experiments were conducted to evaluate the effect of three plant spacing (50x30cm, 60x30cm and 70x30cm) on the vegetative and yield parameters of groundnut (Arachis hypogaea L.). The experiments were conducted as a Randomized Complete Block Design (RCBD) during 2013 and the treatments were replicated three times. The results showed that vegetative parameters such as leaflet area, leaf area, number of branches, plant fresh weight, dry fresh weight, number of pods per plant, number of seeds per plant and seeds yield per plant. All vegetative parameters were significantly different at p≤0.05 among the spacing. The highest leaflet area, leaf area, number of branches, plant fresh weight, dry fresh weight, number of pod per plant, number of seeds per plant and seeds yield per plant were produced at 60x30cm. Regression analyses of seeds yield with vegetative growth parameters revealed a moderate positive relationship of seeds yield with leaflet area (R2=0.81), leaf area (R2=0.71), No. of branches per plant (R2=0.74), and plant fresh weight (R2=0.58), plant dry weight (R2=0.68). Regarding yield components, seeds yield was found to have a moderate or strong positive relationship with No. of pods per plant (R2=0.62) and number of seeds (R2=0.96).

Keywords: Groundnut,Growth,Plant spacing,Yield

7. Exploring chemical composition and genetic dissimilarities between maize accessions, Maydica, Volume:65 Issue-1, June. 2020

  • ABSTRACT

The capacity of maize (Zea mays L.) accessions to tolerate drastically extreme conditions in Iraq, contributes to the characterization of the genetic resources for germplasm management and the identification of the finest genotypes for genetic improvement. Therefore, breeding maize program requires knowledge of genetic variation and genetic structure. A total of 25 maize accessions from three regions (Iraq-Sulaimani, Iraq-Erbil and Iran-Sanandaj) were genotyped by chemical and phytochemical components and simple sequence repeats (SSR) markers to evaluate genetic diversity, population composition and the relationships between genetic and chemical composition dissimilarities. In terms of proximate and phytochemical parameters, the maize accessions exhibited large significant disparity, in which oil, phenol contents and 2,2-diphenyl-1-picrylhydrazyl (DPPH) characteristic appeared to be the most discriminating features of maize accessions. Altogether, 18 SSR markers produced 77 polymorphic alleles across the 25 samples, and the chosen SSR was extremely informative with polymorphic information content (PIC) varied from 0.91 (Bnlg1890) to 0.37 (Umc1630 and Bnlg1189), as well as gene diversity (ranging from 0.48 to 0.91, with an average of 0.75) illustrating the broad genetic variability of the accessions investigated. Molecular variance assessment (AMOVA) showed that there was only 21% genetic variation among populations. Pairwise PhiPT distance (0.10 to 0.31) stated high population distinctions among the populations investigated. In addition, the accessions from three regions were differentiated into seven clusters by both methods; clustering and population structure analysis and the accessions are not grouped in term of geographic locations. Both chemical composition and SSR markers differentiated 25 maize accessions. The results of the Mantel test exhibited a significant positive linkage between chemical components and SSR matrices. The results of this research revealed that maize accessions have a broad genetic diversity that provides a source of new and unique alleles that are helpful for maize breeding programs to address the continuing and future significant challenges and determining collections of well-known cultivars and disparities between them.

8. Effect of Magnetic Field Treatments on Seedling Growth Characteristics of Some Pea (Pisum sativum) Genotypes, Journal of Plant Production, Volume:11 Issue-7, 2020

  • ABSTRACT

In this study, the effects of exposing pre-sown pea seeds to magnetic fields on plant growth parameters have been studied under field conditions. Seeds from five different varieties (Javor, Desier, Mamoth, Oregon Sugar Pod, and Purple Pod) were batch exposed to static magnetic fields for 15, 30, and 45 minutes. Then, the magnetically treated seeds were sown in plastic cups and watered under running water. After 30 days of germination, three plants were marked for the collection of morphological data, different plant growth parameters were tested based on normal seedlings, such as root length, shoot length, fresh root weight, dry weight root and shoot dry weight. The results obtained indicated that the fresh weight of the root, the fresh weight of the shoot and the dry weight of the shoot for the varieties were significantly affected by the time of exposure to the magnetic field, as the duration of the magnetic fields increased. All seeds pretreated by magnetic field showed higher root weight, fresh shoot and dry weight compared to the control condition, and the highest root weight and fresh shoot was reported by treating seeds for 45 minutes under magnetic field conditions. However, the highest shoot dry weight was reported by seed treatment for 30 min. The reports indicated that the application of magnetic fields improved the growth characteristics of the treated group compared to the unexposed group

MSc Thesis:

Characterization of some varieties of chickpea (Cicer arietinum L.) by magnetic treatment, RAPD and ISSR fingerprinting

Books:

Conference Papers