Technology Research and Development Projects

Dual Axes Confocal Endomicroscopy

We are developing the dual axes design to achieve optical sections with high spatial resolution and deep tissue penetration. Confocal endomicroscopy is an emerging imaging technique that can be used to collect histology-like images to provide an instantaneous “optical biopsy.” This technology is useful for performing point-of-care diagnosis where histopathology is performed in real time while the patient is in the clinic.

Multi-photon Endomicroscopy

We are developing miniature imaging instruments to collect multi-photon excited fluorescence in real time to study mouse models of human disease and for clinical imaging with deep tissue penetration. Our approach uses an ultra-fast laser to produce images with sub-cellular resolution and excellent depth while minimizing photodamage and reducing tissue scattering.

Key Publications

In this unique optical design, two beams are oriented at an angle to main optical axis to provide separate paths for illumination and collection. This configuration reduces the effects of scattering, increases dynamic range of detection, and provides deeper images in tissue. Vertical sections can be collected that provide "histology-like" images of tissue in vivo.

We have developed a monolithic 3-axis scanner that provides large out-of-plane motion of the gimbal frame supporting the mirror in the axial direction to achieve deep vertical cross-sections (XZ). This device provides 3D images in vivo.

We have designed a 2D MEMS mirror based on the principle of parametric resonance where large mechanical scan angles can be achieved by driving the structure at integer fractions of the natural frequency.

5 mm diameter dual axes confocal endomicroscope is seen passed through instrument channel of medical endoscope to perform "optical biopsy" in the digestive tract.

Confocal fluorescence images of human colon collected ex vivo with dual axes confocal endomicroscope in vertical plane (XZ) is shown with depth of 430 microns and field-of-view of 1000 microns. A peptide specific for EGFR show spatial distribution of target expression.

Flexible fiber optic side-view instrument can be used to perform repetitive imaging in colon of genetically engineered mice.

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