Production and molecular engineering of Monoclonal Antibody, TIF Antibody, CrossMAb antibody. The purpose was to generate cancer drug therapy using Drug Conjugated Antibody.
Monoclonal Antibody production was made specifically against Pancreatic and colorectal cancer cells marker, CA19-9.
Cell culture: HEK 293 for antibody expression and production.
Colo 205 bearing Ca199 biomarker for antibody binding test.
Preparing and maintaining cell culture and harvesting.
Flow Cytometry to test binding ability of antibody to cancer cell culture bearing
CA199 (Colo 205).
ELISA to measure the expression level of monoclonal antibody.