Activities

PRACTICAL EXPERIENCES:

• • Human tissue culture experience including cancer cells culture and maintenance plus cell transfection with plasmid vectors and cell infection with Lentivirus vector.

  • Murine embryonic stem cells culture and maintenance plus cell transfection, infection, and electroporation.

  • Molecular Biology Techniques including: Polymerase chain reaction (both conventional PCR & qRT-PCR), Flow Cytometry, and Immunocytochemistry. Western blotting analysis, Immunoprecipitation (IP), Immunofluorescent (IF), and Chromatin IP (ChIP) assays. Luciferase, Wound Healing, and Migration assays.

  • Gene cloning and tagging with specific epitos (e.g. Flag, Myc, and EGFP) and site directed mutagenesis using both restriction enzymes and various PCR techniques including overlapping PCR.

  • Experience of T cell isolation from blood and labelling with specific antibodies plus flow cytometry and data analysis.