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There is a large population of walleye in Saginaw Bay. This population is important both ecologically and economically. This project seeks to determine the genetic variation and substructure of this population. The technique that will be employed is DNA fingerprinting. This is essentially the same technique that is used for individual identification by the FBI. This information will be important for determining the long-term viability of the population and the degree of spawning site fidelity, as well as informing future management decisions regarding stocking and dam removal programs.
The project will involve four phases. First, we will use the institute boat to go out onto Saginaw Bay and fish for walleye, using fishing equipment that was purchased for the project last year. Second, we will extract DNA from fin clips collected from the bay and from spawning populations in the spring. Third, we will perform DNA fingerprinting which will include PCR and capillary electrophoresis. Fourth, we will analyze the data and compare it to data obtained in previous years for both the bay and spawning populations.
Participants will be trained in modern moleculargenetic techniques, as well as data analysis. The project may also involve collection and DNA fingerprinting of phragmites and zebra mussels from the Saginaw Bay watershed, in order to determine the genetic diversity and substructure of these invasive species and to determine the genetic effects of eradication programs.