Seri-Biotechnology
(SBT-321) (2+1)

Course Developer:

Dr. Firdose Ahmad Malik

Assistant Professor

COTS Mirgund

Dr. Shabir Ahmad Bhat

Assistant Professor (SS)

COTS Mirgund

Lecture 1: Introduction To Biotechnology In Sericulture

  • Introduction to Biotechnology in Sericulture

  • Principles and Methods Used in Biotechnology

  • Impact of Biotechnology on Sericulture

Lecture 2: Plant Tissue Culture In Mulberry

  • Plant Cell and Tissue Culture Techniques in Mulberry

  • History of Plant Tissue Culture

  • Different Techniques and Methods of Plant Tissue Culture

  • Principles of Tissue Culture

  • Applications of Tissue Culture in Mulberry

Lecture 3: Micropropagation; Somatic Embryogenesis, Somaclonal Variations & Synthetic Seeds In Mulberry

  • Micropropagation of Mulberry

  • Micropropagation Vs. Conventional Method of Propagation

  • Stages of Micropropagation

  • Somatic Embryogenesis in Mulberry

  • Somaclonal Variations in Mulberry

  • Applications in Mulberry

Lecture 4: Development Of Haploids & Triploids In Mulberry & Protoplast Culture In Mulberry

  • Development of Haploids in Mulberry

  • Development of Triploids in Mulberry

  • Protoplast Culture in Mulberry

Lecture 5: Recombinant Dna Technology And Its Applications In Sericulture

  • Introduction to Recombinant DNA Technology

  • Principles of Recombinant DNA Technology

  • Applications of Recombinant DNA Technology in Sericulture

Lecture 6: Gene Transfer Methods & Techniques Using Agrobacterium In Mulberry

  • Introduction to Gene Transfer Methods in Mulberry

  • Agrobacterium-Mediated Genetic Transformation

  • Structure of Ti Plasmid

  • Steps Involved in Agrobacterium-Mediated Genetic Transformation of Plants

  • Genetic Transformation of Mulberry

Lecture 7: Selectable & Scanable Markers

  • Selection of Transformants

  • Selectable Marker

  • Examples of Selectable Markers

  • Scanable Marker/ Screening Marker

  • Types of Screening Markers

Lecture 8: Genomic Organization Of Bmnpv & Bmnpv Vector

  • Introduction to Bombyx Mori Nuclear Polyhedrosis Virus (Bmnpv)

  • Life Cycle of Bmnpv

  • Genomic Organization of Bmnpv

  • Bmnpv Vector

Lecture 9: Biotechnological Application For Large Scale Synthesis Of Recombinant Proteins (Valuable Proteins) Using Bmnpv

  • Introduction to Synthesis of Recombinant Proteins Using Bmnpv

  • Silkworm Genome Sequence and Its Relation with Baculovirus Expression System

  • Baculovirus Expression System in Silkworm Larvae or Pupae

  • Recombinant Bombyx Mori Nucleopolyhedro Virus Harboring Green Fluorescent Protein

  • Bmnpv Bacmid Development and Its Application

Lecture 10: Polymerase Chain Reaction (PCR): Gene Amplification, Application Of PCR In Seri- Biotechnology

  • Introduction to Polymerase Chain Reaction

  • Components of PCR Reaction

  • Gene Amplification

  • Variants of PCR

  • Applications of PCR In Seri Biotechnology

Lecture 11: Nanotechnology And Its Applications In Sericulture

  • Introduction to Nanotechnology

  • Biosynthesis of Nanoparticles

  • Applications of Nanotechnology in Sericulture

Lecture 12: Bioinformatics & Its Applications In Sericulture

  • Introduction to Bioinformatics

  • Scope of Bioinformatics

  • Activities in Bioinformatics

  • Aims of Bioinformatics

  • Applications of Bioinformatics in Sericulture

Lecture 13: Marker Assisted Breeding In Improvement Of Host Plants And Silkworm

  • Introduction to Marker Assisted Selection

  • Advantages and Limitations of Marker Assisted Selection

  • Applications of DNA Markers in Mulberry Breeding

  • Applications of DNA Markers in Silkworm Breeding

LECTURE 14: IPR (INTELLECTUAL PROPERTY RIGHTS), PATENTING, WTO-GATT AND BIOETHICS

  • IPR (Intellectual Property Rights)

  • GATT (General Agreement on Tariffs and Trade)

  • WTO (World Trade Organization)

  • Bioethics