UROX SoftwareInteractive fitting of atomic models into Electron Microscopy reconstructions (and beyond...)
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Downloads (32-bits Linux Binaries -- for now...)
Installation
- tar -zxvf urobin_{version_number}.tgz
- cd UROX
- source installbin_{version_number}.[c]sh (for csh or for sh)
- then type 'urox' and enjoy (watch this video for a quick outlook)
Requirements
Publications
- X. Siebert and J. Navaza : "UROX 2.0 : An interactive tool for fitting atomic models into Electron Microscopy reconstructions" Acta Crys (2009) D65, 651-658
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J. Navaza et al. : "On the fitting of model electron densities into EM reconstructions: a reciprocal-space formulation", Acta Cryst. (2002). D58, 1820-1825
Recent Publications using/citing UROX (incomplete list, please let me know if yours is not included !) :
- Chang et al. " Reconstructing Virus Structures from Nanometer to Near-Atomic Resolutions with Cryo-Electron Microscopy and Tomography", Advances in Experimental Medicine and Biology, Volume 726, 49-90 (2012)
- Elegheert et al., "Extracellular Complexes of the Hematopoietic Human and Mouse CSF-1 Receptor Are Driven by Common Assembly Principles", Structure 19(12) 1762-1772 (2011)
- Aramayo R. et al., "Quaternary structure of the specific p53–DNA complex reveals the mechanism of p53 mutant dominance" Nucl. Acids Res. (2011)
- O. Pilak et al " Chaperonins from an Antarctic archaeon are predominantly monomeric: crystal structure of an open state monomer", Environmental Microbiology (2011)
- M L. Baker et al. Modeling protein structure at near atomic resolutions with Gorgo, J. Struct. Biol (2011)
- W. Wriggers et al. Matching structural densities from different biophysical origins with gain and bias (2011), J. Struct. Biol. 173(3), 445-450
- M. Beak et al. Exploring the spatial and temporal organization of a cell's proteome, J. Struct. Biol (2011), 173(3) :483 - 496
- N. Voss et al. Software Tools for Molecular Microscopy: An Open-Text Wikibook, Methods in Enzymology (2010) 482:381-392
- M L. Baker et al. Analyses of Subnanometer Resolution Cryo-EM Density Maps, Methods in Enzymology (2010) 483: 1-29
- Libersou S. et al. Distinct structural rearrangements of the VSV glycoprotein drive membrane fusion,(2010) J Cell Biol 191:199-210.
- Baker ML et al., Analyses of Subnanometer Resolution Cryo-EM Density Maps, Methods in Enzymology, Academic Press (2010) Volume 483, Cryo-EM, Part C: Analyses, Interpretation, and Case studies, Pages 1-29.
- J-K Hyun, Mazdak Radjainia, Richard L. Kingston, Alok K. Mitra, Proton-driven Assembly of the Rous Sarcoma Virus Capsid Protein Results in the Formation of Icosahedral Particles J. Biol. Chem. (2010) 285: 15056-15064.
- He Y, Olson R. Three-dimensional structure of the detergent-solubilized Vibrio cholerae cytolysin (VCC) heptamer by electron cryomicroscopy., J Struct Biol. (2010), 169(1):6-13.
- Okorokov AL, Chaban YL, Bugreev DV, Hodgkinson J, Mazin AV, et al. Structure of the hDmc1-ssDNA Filament Reveals the Principles of Its Architecture. PLoS ONE ( 2010), 5(1)
- Sciara G. et al. Structure of lactococcal phage p2 baseplate and its mechanism of activation, P.N.A.S. (2010) 107 (15) 6852-6857
- Martin D, Duarte M, Lepault J, Poncet D. Sequestration of Free Tubulin Molecules by the Viral Protein NSP2 Induces Microtubule Depolymerization during Rotavirus Infection. J.Virol (2009)
- S. Muench et al. Cryo-electron Microscopy of the Vacuolar ATPase Motor Reveals its Mechanical and Regulatory Complexity, J Mol Biol (2009), 386 (4), 989 - 999.
- Yongning He and Rich Olson, Three-dimensional structure of the detergent-solubilized Vibrio cholerae cytolysin (VCC) heptamer by electron cryomicroscopy, Journal of Structural Biology (2009)
- Lhuillier et al. "Structure of bacteriophage SPP1 head-to-tail connection reveals mechanism for viral DNA gating", P.N.A.S. (2009) 106 (21), 8507-8512
- Suman Lata, et al. "Helical Structures of ESCRT-III Are Disassembled by VPS4", Science 321, 1354 (2008)
- Hui Li, et al., "A Neoplastic Gene Fusion Mimics Trans-Splicing of RNAs in Normal Human Cells", Science 321, 1357 (2008);
- Dong-Hua Chen et al. "Location and Flexibility of the Unique C-Terminal Tail of Aquifex aeolicus Co-Chaperonin Protein 10 as Derived by Cryo-Electron Microscopy and Biophysical Techniques", J. Mol. Bio. (2008); 381 (3), 707-717
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Mazdak Radjainia, Yu Wang and Alok K. Mitra, Structural Polymorphism of Oligomeric Adiponectin Visualized by Electron Microscopy, Journal of Molecular Biology Volume 381, Issue 2, Pages 419-430 (2008)
- Libersou S., Siebert X., Ouldali M., Estrozi L. F., Navaza J., Charpilienne A., Garnier P., Poncet D. and Lepault J. ``Geometry mismatches within the concentric layers of rotavirus particles: a potential regulatory switch of the rotavirus transcription activity'', J. Virol. (2008); 82(6):2844-52
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Gutsche I, Vujičić-Žagar A, Siebert X, Servant P, Vannier F, Castaing B, Gallet B, Heulin T, de Groot A, Sommer S, Serre L.
Complex oligomeric structure of a truncated form of DdrA: A protein required for the extreme radiotolerance of Deinococcus., Biochim Biophys Acta. 2008 Apr (see also the commentary in Nanoprobes) -
Medical-Imaging-Week : news on UROX
Links
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NORMA (normal mode fitting)
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EM Data Bank
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Wikipedia Software tools for Electron Microscopy (including UROX)



