Zoinks! Two years after a clash of egos forced Mystery Inc. to close its doors, Scooby-Doo and his clever crime-solving cohorts Fred (Freddie Prinze Jr.), Daphne (Sarah Michelle Gellar), Shaggy (Matthew Lillard) and Velma (Linda Cardellini) are individually summoned to Spooky Island to investigate a series of paranormal incidents at the ultra-hip Spring Break hot spot.

The Mystery Inc. team is invited at the grand opening of the Coolsville Museum of Criminology, displaying costumes of the monsters. However, a masked man creates havoc by stealing those costumes.