Uv-vis Analyst Software Download

The Solo VPE system greatly reduces those concerns. In nearly all cases, it can measure concentration without the need for dilution, which eliminates analyst-related errors associated with that part of sample preparation. The only analytical error thus associated with the assay is that related to the instrument itself, commonly referenced as 2% (3). Similarly, a simplified sample preparation coupled with the rapid scan time of the Solo VPE system generates data within minutes rather than hours. That helps to reduce overall product-processing times, and in turn it reduces production costs.

All those preparations can introduce additional errors, sometimes on the order of several percent. Errors associated with sample preparation vary based largely on analyst expertise, which creates a need for stringent analyst training and qualification programs. Analysts may struggle for weeks trying to develop the technique necessary to perform a traditional A280 analysis and generate accurate, repeatable results.

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Our results illustrate the comparatively low level of variance associated with changes in instrument, analyst, and day of analysis on the Solo VPE system. Much variance associated with results obtained from the standard UV-vis are probably related to the required performance of dilutions. In fact, because dilutions are not made when using the Solo VPE system, assessment of analyst-to-analyst variability during routine method validation is no longer meaningful.

In our study, we realized significant efficiency gains through the use of the Solo VPE system. Because slope-spectroscopy analysis with a variable-pathlength system requires no sample preparation or dilution, a substantial amount of time is saved without changing any other aspect of the assay. With most traditional A280 methods, samples are analyzed in triplicate to average out the variability associated with the sample preparation and dilution performed by analysts. Because it is no longer necessary to compensate for that variability, analysis of replicate sample preparations may be reduced or eliminated.

One of the aims in the forensic analysis of physical evidence is to compare a questioned material from a crime scene with a specific reference, for example from a suspect, to determine whether the two samples are indistinguishable. The examination of coloured materials such as inks and fibres falls into this category and presents a significant challenge to the forensic analyst who must determine whether the particular shades exhibited by the pair of samples may be discriminated or not.

UV/visible spectroscopy plays a key role in the discrimination of colour in the forensic analysis of fibres and inks. The application of chemometrics, however, is vital in many cases to enhance such discrimination and to put it on a quantitative basis so providing objective justification for the conclusions of the analyst.

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