Proteomics is the global analysis of proteins in a sample, and its methodologies are commonly applied in life science research. Despite its wide applicability however, proteomics is rarely used as a tool in criminal investigations. Here we present a case where the technique provided key evidence in a case that involved the death of a two-year old girl. The defendant was known to keep exotic snakes, including several venomous species, which led the coroner to probe whether there could be snake venom in the blood of the deceased. One major challenge of the investigation was the overwhelming presence of several blood proteins, such as apolipoprotein and complement proteins, which hinders the detection of less abundant analytes. In a counter-acting strategy, a combination of immunodepletion and fractionation methods was used; the sample was then submitted to tandem mass spectrometry for peptide identification. Using this strategy, 15,000 peptides could be sequenced. However, the subsequent challenge was to differentiate between human and snake proteins, given the genetic similarities that are shared by the two vertebrate species. After a thorough bioinformatics search and manual inspection, we found that

"The recording process was very beautiful and full of trust. My role, Snake Girl, was a challenge for me at the reading itself, and especially later when it turned out to be very complex to play. I think that she is a symbol of the unknown, new and exciting, with all the consequences it carries," says Kordi.


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Aunty tayua went to visit her friend in her house,to discuss some personal issues bodereing her,she needed advise from her friend and the two had been friends from childhood,but unknown to tayua ,fosua her friend has been going to a native herbalist man for different kinds of sacrifices she claimed as protection,long life and the rest.

I remember seeing these small shows happening in Baada, Hegde and Kumta fairs when I was kid. They used to show three legged girl, girl with a three eyes and some magical stuff which were a big attraction of a rural fairs in those days. Four to five tents which showed these amazing things for day and almost till midnight for continuous three to six days. For the people visiting fairs these shows happened to be one of the biggest attractions. I remember discussing the shows with my friends in schools or while playing cricket even after the fair is over. But as I am in to my college I learnt that these are just tricks so I stopped going to these shows , I think I stopped going to these fairs and justified that these are village events for village people!

Now it is 10 years after my college and these fairs are attracting me again. I am squeezing my times between my worklife to visit these village fairs again. I see a great culture that has come a long way . When I was in Hegde fair this year I saw this similar show and got interested right away. They were showing body less girl, a girl with a snake body , floating stone, a tap flying in air with continuous water flow. I said WOW! So I decided I have to see this as a grown up. It was not open at that time . So I interacted with the show guys to know more about them.

I could see those girls smiling at us looking here and there but anybody can tell with little observation that it is a fake smile she is wearing. One can see there was a little pain behind it. One can see her neck muscles stretched and the posture they are in is not a comfortable one.

I do not want to debate whether those girls really have just head or a snake body for now. Technically all I could think is may be those girls were in such a posture which fools the viewer . May be those girls are disabled with a small body or something . But the interesting / painful thing is those girls were there in that posture for hours without eating anything or drinking. And it is not for one day. It is continuous! Once this fair is over they move to another fair and put their tent . They perform day and night to earn money for their owners and their family. So much to do for just few earnings!! But it is common in India which is having such a population.

The tale of the deadly snake lurking in consumer goods dates to at least the mid 1960s. As a snippet of popular lore, it combined two familiar elements: distrust of business establishments, and dangerous foreign creatures encountered in unexpected places.

Recently, when visiting my parents in NJ, my elderly aunt told me that her neighbor's visiting nurse told her that another patient of hers was recovering from a snakebite. This woman had gone to a large clothing outlet type store and tried on a coat. After experiencing swelling and discomfort in her shoulder, went to the doctor who pronounced her the victim of an unknown snake. She returned to the store, found the same coat she had tried on and the store manager discovered two baby snakes still in residence in the coat. The woman had recovered and of course was suing the store.

While going through this procedure, all of a sudden, she gave a cry and fell to the floor. She was immediately rushed to hospital but found to be dead on arrival. On examination it was discovered that she had died as a result of a snake bite. When the baskets were checked they found a large and deadly poisonous snake in the bottom of the one she was seen to examine last. It was concluded that the snake had arrived in America having been shipped in the basket from the Far East.

"If a snake is in there, it's got to sit in a distribution center for a few months before it's sent to the stores. Are you going to tell me, it survived all that time up a sleeve?" said Ric Bramble, Burlington Coat Factory spokesman at the chain's headquarters in Burlington.

Snake sightings in retail establishments are even more rare, but a 1991 lawsuit asserted that one slithered into a Pennsylvania couple's mattress. Shortly after purchasing the mattress in May 1988, the couple noted unusual movements in the bed that they suspected could be a living creature. They exchanged the mattress for another, but again noted those same odd movements in their bed. After four months of this, they took the mattress to a lab, where workers found what was left of a 26-inch ribbon snake. The couple asked for $20,000 in damages.

In September 2003, Douglas Hatchett of Bangs, Texas, while shopping for shoes in a Wal-Mart, claimed to have been bit by a rattlesnake which had been concealed behind a shoebox on a shelf. The victim said he stomped the snake to death, and the man was treated and released the same day from an area hospital. Doubt has since been expressed as to the veracity of his claim, with the hospital asserting Hatchett was not bitten by the snake and that their services were stolen by him. Wal-Mart as well is questioning the validity of Hatchett's story, and police at the scene said to a Wal-Mart employee the dead Western Diamondback rattler didn't look like a freshly killed snake.

Most of all, however, this tale speaks to our fear of foreign contamination. The snake in this legend always comes from outside the U.S.A., even though America also has both venomous vipers and a textile industry. Just as in the Mexican Pet and the spider-filled shaking cactus legends, something brought in from a foreign land conceals untold dangers.

Despite their resemblance to terrestrial snakes, the Viper is actually a wholly extraterrestrial species unmodified by human DNA. However, it retains the modified Thin Man's poison and high accuracy, combat abilities and intelligence.

Hopi-Tewa potter. Name variations: Nampayu; The Old Woman; Snake Woman, Snake Girl or Tsu-mana. Born Nampeyo on the Hopi First Mesa called Hano, northeast Arizona, around 1860; died on July 20, 1942, in Hano; daughter of Kotsakao, also called Qotca-ka-o (a Tewa woman of the Corn Clan), and Kotsuema also called Qots-vema (a Hopi man of the Snake Clan); married Kwivioya, in 1879 (marriage annulled, date unknown); married Lesou, in 1881 (died 1932); children: (second marriage) four daughters, Kwe-tca-we, Ta-wee, Po-pong-mana, and Tu-hi-kya; one son, Qoo-ma-lets-tewa (died 1918).

Belinda has a face with patterned white scales topped by a head of snow-white hair and scarlet eyes. She is slender and evidently a demi snake, that has descended from the Alabaster Devilsnake. She has a sharp voice. She has a distinct aura but not seeming sinister. These features paired well with her air of superiority, giving her a unique charm.

Early access to medical care in a health facility that has personnel trained and capable of diagnosing snakebite envenoming is essential. This means, a health centre which is equipped with the basic resources needed to provide immediate emergency treatment needs, including the administration of antivenom and other adjunct therapy.

Improving the clinical outcomes for the victims of snake bite needs much more than just access to safe antivenoms. Intravenous access should be achieved early, hydration state determined and corrected if needed, and vital signs must be closely monitored. The early administration of an adequate dose of effective antivenom to patients with signs of envenoming is crucial. If no antivenom is available, referral to a centre which has supplies should be planned and undertaken quickly. If this is not possible then symptomatic treatment including support of airway patency and breathing, maintenance of circulation and control of bleeding, and the treatment of local wounds should be prioritized as appropriate.

Administered early, antivenoms are not just life-saving, but can also spare patients some of the suffering caused by necrotic and other toxins in snake venom, leading to faster recovery, less time in hospital and a more rapid transition back to a productive life in their communities. But the reality for many patients is that early access to antivenom is simply not possible for a multitude of reasons (see Box 2). As a consequence, these patients do not receive the full potential benefit of antivenom, and some of the effects of the snake venom may not be neutralized effectively, leading to prolonged illness, slower recovery and greater risk of disability. For those affected by toxins that cause paralysis, sustained airway and breathing assistance using either manual resuscitators or mechanical ventilators may be necessary. Patients bitten by snakes with venom that affects normal blood clotting may have a higher risk of internal bleeding into the brain and other organs, and those affected by dermonecrotic toxins will experience more severe local tissue damage. e24fc04721

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