The FlexCam iCam Digital from Ken-A-Vision is an economical and versatile camera that is well suited for a variety of tasks. Present objects, images, or text. Send video e-mail or create video portfolios. This unit is also perfect for viewing microscopic images by using its integrated focus knob and included microscope adapters.
The unit includes a solid base and a flexible arm with the camera mounted at its end. Bend and twist the arm to perfectly position the camera to meet your needs. It can be connected to a PC, Mac, television, VCR, or LCD projector. Also included is ArcSoft Video Impression Photo Studio software to facilitate image & video capture and editing.
The Dino-Lite AM4111T handheld digital microscope is featured with the unique MicroTouch snapshot feature and comes with a high quality 1.3 megapixel image sensor that views and takes highly detailed images for professionals.. The MicroTouch (touch...
In addition, the microscope features the industry leading ergonomic advances for which Nikon is celebrated. Controls are located so that hand movements are instinctive, eliminating the need to reach forward and twist or lift arms from the table. The fine focus knob and the stage handle can be controlled with one hand.
The twisted pharynx phenotype in mnm-4;etIs2 worms of different stages, and in adult mnm-4;unc-61. (A) An example of a twisted pharynx and the three measurements that can be obtained by DIC microscopy to estimate the actual degree of twist within the isthmus using the formula shown on the right side (D is diameter; L is isthmus length; and θ is the angle between the torsion lines and the pharyngeal axis). (B) Analysis of etIs2 [pRF4 pRIC-19::GFP] transgenic worms [12]: DIC images (left column), M2 neurons of the same worms visualized via their GFP expression (middle column), and the average degree of twist within the isthmus for at least three similar worms scored using confocal microscopy (pie charts). Genotypes and stages are as indicated in the DIC images. See Table 1 for actual numerical data and list of alleles. For the confocal microscopy analysis, worms were mounted on dried agarose pads (2% in dH2O), paralyzed with a small drop of 100 mM levamisole and covered with a coverslip. The worms were examined using a Zeiss LSM 510 META system connected to an inverted Zeiss Axiovert 200 microscope. The z-stacks were projected in 360 using 32 or 64 steps and then exported as full resolution images in avi or mov format using the in-microscope software LSM 510 ConfoCor2 Combination, version 3.2. These movies were then used to determine the degree of twisting in the isthmus using video editing software (Sorenson squeeze, trial version). "180 twist" means that the distal ends of the M2 neurons would have to be rotated by 180 in order to be parallel with the cell bodies from which they originate.
Description of isolated pharynges and abnormal pharynges. For panels A-F, pharynges were dissected from worms held on dried agarose pads (2% in H2O) covered with mineral oil and using a tungsten (0.5 mm) dissecting needle, then transferred to a drop of M9 on an agarose pad (2% in M9), allowed to relax by swirling the liquid before applying a coverslip, and examined under the microscope. Note that the control pharynx of wild-type worms carrying the etIs2 integrated sequence is straight, and that the isolated pharynges of mnm-4;etIs2, mnm-4, mig-4 and unc-61 worms are twisted whereas the pharynx of the dig-1 animal is untwisted. Panels G-J show the pharyngeal twist in mnm-4 animals carrying mutations that cause abnormal pharynx morphologies (pha-2, sma-1) or reduced pumping rate (eat-3). Worms in G and H are adults, and L4 larvae are shown in I-J. Note that the enlarged procorpus of mnm-4;sma-1 worms and the enlarged isthmus of mnm-4; pha-2 worms show less twisting then the corresponding part of control worms, and that the mnm-4 is slightly more twisted than the eat-3;mnm-4 worm.
Anatomical features of the head region and comparison of the hemicentin-rich pharyngeal tendons between wild-type and a mutant with the twisted pharynx phenotype. (A) and (B) show transverse and cross sections of the head region in idealized forms. For clarity, many structures were omitted here, including axons, excretory canals, muscle arms and complex hypodermal cell shapes that sometimes cover the body muscles. Of particular importance is that the pharynx seems to float in pseudocoelomic fluid and to make almost no contact with the worm body along its entire length: except for the tendons, the pharynx is secured only at its anterior and posterior ends, where it is connected to the mouth and intestine, respectively. (C) Transverse thin section of an adult wild type nose, showing a left ventral tendon (red arrows) connecting the basal laminae (red arrowheads) of the pharyngeal epithelium (PH) and of the body-wall muscles (BWM). Major cells bordering the tendon include the amphid sheath cell (AMSh) and several other sheath cells (Sh) for mechanosensors of the lips. Smaller caliber processes include many sensory dendrites and some arcade processes. Hemidesmosomes link the pharyngeal epithelium's intermediate filaments to the basal lamina. Dense bodies (modified adherens junctions) link the muscle sarcomeres to the muscle's basal lamina. Because it is tilted with respect to the body axis, the tendon is better seen close to the pharynx in this image, but goes out of the plane of section as it passes between the muscle cells. Image is rotated about 20 degrees clockwise for convenience. Scale bar is 1 μm. (D) and (E) show images of a wild-type and mnm-4 mutant that carry the hemicentin::GFP transgene rhIs23 [30], respectively. (F) and (H): geometry of the tendons (brown) and pharynx (blue circle) viewed in cross sections if the pharynx is not twisted (F) or if it were twisted as a whole while held by the tendons (H). (G) and (I) show cross section views of the flattened confocal image stacks from (D) and (E); note the spiral-oriented tendons in (I). Specimens were immersion fixed using buffered aldehydes and then osmium tetroxide as described previously [40]. Three or four animals were aligned within agar blocks then embedded in plastic resin and sectioned together. Thin cross sections were collected on slot grids, post-stained with uranyl acetate and lead citrate, then examined with a JEOL 1200EX electron microscope. Scale bars in D and H are 10 μm.
For microscopic analysis, worms were mounted on 2% agarose pads, paralyzed with a small drop of 100 mM levamisole, and covered with a coverslip. These were then examined with a Zeiss Axioplan compound microscope using DIC optics or an FITC filter set to visualize GFP. Digital images were acquired using an attached AxioCam digital camera.
Worms expressing pRIC-19::GFP in their M2 neurons were mounted ondried agarose pads (2% in dH2O), paralyzed with a small drop of 100 mM levamisole and covered with a coverslip. The worms were examined using a Zeiss LSM 510 META system connected to an inverted Zeiss Axiovert 200 microscope. The z-stacks were projected in 360 using 32 or 64 steps and then exported as full resolution images in avi or mov format using the in-microscope software LSM 510 ConfoCor2 Combination, version 3.2. These movies were then used to determine the degree of twisting in the isthmus using video editing software (Sorenson squeeze, trial version). Worms carrying pMH86 was examined using BioRad radiance 2000 setup. The z-stackswas projected in 360 in 36 steps using the in-microscope software Laser Sharp 2000 and thereafter scored using the image processing program ImageJ. "180 twist" means that the distal ends of the M2 neurons would have to be rotated by 180 in order to be parallel with the cell bodies from which they originate.
The cancer researchers are interested in whether switching off the production of certain proteins can inhibit the transport mechanisms which are important for the filopodia of cancer cells. According to Bendix, the mechanical function of filopodia can be compared to a rubber band. Untwisted, a rubber band has no power. But if you twist it, it contracts. This combination of twisting and contraction helps a cell move directionally and makes the filopodia very flexible.
Dino-Lite MSAA111A2 - for Dino-Lite ZTA series
Diffused illumination plays an important role in digital microscopy. The Dino-Lite line of diffusers work to soften the Dino-Lite's bright LED illumination by having it pass through the semi-translucent cone before striking the object.
This has the effect of reducing shadow contrast while bringing out more surface detail. Diffused illumination also aids in greatly reducing eye fatigue during extended periods of observation.
We have a full lineup of diffusers available for your specific Dino-Lite microscope. Please reference the appropriate model number below.
MSAA111A1 - For Dino-Lite AM series (except ZT models or AM4013MT)
MSAA111A2 - For Dino-Lite ZT series
MSAA111A3 - For Dino-Lite ZTS series
MSAA111A4 - Spacing Adapter for AM413ZTS series
MSAA112B - For Dino-Lite AD Series (except "Z" or "L" models)
Proper installation of the diffuser: Place the diffuser into the clear plastic nozzle. Apply slight pressure until diffuser is secure. Please be cautious of misaligned Led lights.
Proper removal of the diffuser: While holding the microscope with one hand, grip the clear plastic nozzle of the microscope with the other hand and gently twist and remove the diffuser.
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