論文・出版物

 原著論文

Hiyoshi H, Sakuma K, Asano S, Napier SC, Konagaya S, Mochida T, Ueno H, Watanabe T, Kassai Y, Matsumoto H, Ito R, Toyoda T. Identification and removal of unexpected proliferative off-target cells emerging after iPSC-derived pancreatic islet cell implantation. Proc Natl Acad Sci U S A. 2024 Apr 16;121(16):e2320883121. doi: 10.1073/pnas.2320883121. （概要はこちら）


Ito R, Kimura A, Hirose Y, Hatano Y, Mima A, Mae SI, Keidai Y, Nakamura T, Fujikura J, Nishi Y, Ohta A, Toyoda T, Inagaki N, Osafune K. Elucidation of HHEX in pancreatic endoderm differentiation using a human iPSC differentiation model. Sci Rep. 2023 May 29;13(1):8659. doi: 10.1038/s41598-023-35875-1.


Sakuma K, Tsubooka-Yamazoe N, Hashimoto K, Sakai N, Asano S, Watanabe-Matsumoto S, Watanabe T, Saito B, Matsumoto H, Ueno H, Ito R, Toyoda T. CDK8/19 inhibition plays an important role in pancreatic β-cell induction from human iPSCs. Stem Cell Res Ther. 2023 Jan 5;14(1):1. doi: 10.1186/s13287-022-03220-4.  （概要はこちら）（外部の関連サイト）


Tsujisaka Y, Hatani T, Okubo C, Ito R, Kimura A, Narita M, Chonabayashi K, Funakoshi S, Lucena-Cacace A, Toyoda T, Osafune K, Kimura T, Saito H, Yoshida Y. Purification of human iPSC-derived cells at large scale using microRNA switch and magnetic-activated cell sorting. Stem Cell Reports. 2022 Jul 12;17(7):1772-1785. doi: 10.1016/j.stemcr.2022.05.003. Epub 2022 Jun 9.

 

Hiyoshi H, Sakuma K, Tsubooka-Yamazoe N, Asano S, Mochida T, Yamaura J, Konagaya S, Fujii R, Matsumoto H, Ito R, Toyoda T. Characterization and reduction of non-endocrine cells accompanying islet-like endocrine cells differentiated from human iPSC. Sci Rep. 2022 Mar 18;12(1):4740. doi: 10.1038/s41598-022-08753-5.  （概要はこちら） 

 

Suenaga R, Konagaya S, Yamaura J, Ito R, Tanaka S, Ishizaki Y, Toyoda T. Microwell bag culture for large-scale production of homogeneous islet-like clusters. Sci Rep. 2022 Mar 25;12(1):5221. doi: 10.1038/s41598-022-09124-w. （概要はこちら） 

 

Kimura A, Toyoda T, Iwasaki M, Hirama R, Osafune K. Combined Omics Approaches Reveal the Roles of Non-canonical WNT7B Signaling and YY1 in the Proliferation of Human Pancreatic Progenitor Cells. Cell Chemical Biology. 2020 Dec 17;27(12):1561-1572.e7. doi: 10.1016/j.chembiol.2020.08.018. Epub 2020 Oct 29.

 

Mochida T, Ueno H, Yamazoe N, Hiyoshi H, Ito R, Matsumoto H, Toyoda T. Insulin-deficient diabetic condition upregulates insulin secreting capacity of human iPSC-derived pancreatic endocrine progenitor cells after implantation in mice. Diabetes. 2020 Apr;69(4):634-646. doi: 10.2337/db19-0728. Epub 2020 Jan 31. （概要はこちら） 

~2019

Toyoda T, Kimura A, Tanaka H, Osafune K. Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures. J Vis Exp. 2019 Mar 27;(145). doi: 10.3791/57641.

 

Matsui S, Ochiai M, Yasuda K, Mae SI, Kotaka M, Toyoda T, Yamamoto T, Osafune K. Differentiation and isolation of iPSC-derived remodeling ductal plate-like cells by use of an AQP1-GFP reporter human iPSC line. Stem Cell Res. 2019 Mar;35:101400. doi: 10.1016/j.scr.2019.101400. Epub 2019 Jan 31.

 

Kotaka M, Toyoda T, Yasuda K, Kitano Y, Okada C, Ohta A, Watanabe A, Uesugi M, Osafune K. Adrenergic receptor agonists induce the differentiation of pluripotent stem cell-derived hepatoblasts into hepatocyte-like cells. Sci Rep. 2017 Dec 1;7(1):16734. doi: 10.1038/s41598-017-16858-5.

 

Mae SI, Ryosaka M, Toyoda T, Matsuse K, Oshima Y, Tsujimoto H, Okumura S, Shibasaki A, Osafune K. Generation of branching ureteric bud tissues from human pluripotent stem cells. Biochem Biophys Res Commun. 2018 Jan 1;495(1):954-961. doi: 10.1016/j.bbrc.2017.11.105. Epub 2017 Nov 20.

 

Kimura A, Toyoda T, Nishi Y, Nasu M, Ohta A, Osafune K. Small molecule AT7867 proliferates PDX1-expressing pancreatic progenitor cells derived from human pluripotent stem cells. Stem Cell Res. 2017 Oct;24:61-68. doi: 10.1016/j.scr.2017.08.010. Epub 2017 Aug 17.


Hosokawa Y, Toyoda T, Fukui K, Yamaguchi Baden M, Funato M, Kondo Y, Sudo T, Iwahashi H, Kishida M, Okada C, Watanabe A, Asaka I, Osafune K, Imagawa A, Shimomura I. Insulin-producing cells derived from ‘induced pluripotent stem cells’ of patients with fulminant type 1 diabetes: vulnerability to cytokine insults and increased expression of apoptosis-related genes. J Diabetes Investig. 2017 Aug 10;9(3):481-493. doi: 10.1111/jdi.12727.

 

Toyoda T, Kimura A, Tanaka H, Ameku T, Mima A, Hirose Y, Nakamura M, Watanabe A, Osafune K. Rho-associated kinases and non-muscle myosin IIs inhibit the differentiation of human iPSCs to pancreatic endoderm. Stem Cell Reports. 2017 Aug 8;9(2):419-428. doi: 10.1016/j.stemcr.2017.07.005.

 

Kondo Y, Toyoda T, Inagaki N, Osafune K. iPSC technology-based regenerative therapy for diabetes. J Diabetes Investig. 2018 Mar;9(2):234-243. doi: 10.1111/jdi.12702. Epub 2017 Jul 29.

 

Kondo Y, Toyoda T, Ito R, Funato M, Hosokawa Y, Matsui S, Sudo T, Nakamura M, Okada C, Zhuang X, Watanabe A, Ohta A, Inagaki N, Osafune K. Identification of a small molecule that facilitates the differentiation of human iPSCs/ESCs into pancreatic endocrine cells. Diabetologia. 2017 Aug;60(8):1454-1466. doi: 10.1007/s00125-017-4302-7. Epub 2017 May 22.

 

Yoshitoshi-Uebayashi EY, Toyoda T, Yasuda K, Kotaka M, Nomoto K, Okita K, Yasuchika K, Okamoto S, Takubo N, Nishikubo T, Soga T, Uemoto S, Osafune K. Modelling urea-cycle disorder citrullinemia type 1 with disease-specific iPSCs. Biochem Biophys Res Commun. 2017 May 6;486(3):613-619. doi: 10.1016/j.bbrc.2017.03.037. Epub 2017 Mar 14.

 

Matsuno K, Mae SI, Okada C, Nakamura M, Watanabe A, Toyoda T, Uchida E, Osafune K. Redefining definitive endoderm subtypes by robust induction of human induced pluripotent stem cells. Differentiation. 2016 Dec;92(5):281-290. doi: 10.1016/j.diff.2016.04.002.

 

Ameku T, Taura D, Sone M, Numata T, Nakamura M, Shiota F, Toyoda T, Matsui S, Araoka T, Yasuno T, Mae S, Kobayashi H, Kondo N, Kitaoka F, Amano N, Arai S, Ichisaka T, Matsuura N, Inoue S, Yamamoto T, Takahashi K, Asaka I, Yamada Y, Ubara Y, Muso E, Fukatsu A, Watanabe A, Sato Y, Nakahata T, Mori Y, Koizumi A, Nakao K, Yamanaka S, Osafune K. Identification of MMP1 as a novel risk factor for intracranial aneurysms in ADPKD using iPSC models. Sci Rep. 2016 Jul 15;6:30013. doi: 10.1038/srep30013.

 

Yu H, Fujii NL, Toyoda T, An D, Farese RV, Leitges M, Hirshman MF, Mul JD, Goodyear LJ. Contraction stimulates muscle glucose uptake independent of atypical PKC. Physiol Rep. 2015 Nov;3(11). pii: e12565. doi: 10.14814/phy2.12565.

 

Toyohara T, Mae S, Sueta S, Inoue T, Yamagishi Y, Kawamoto T, Kasahara T, Hoshina A, Toyoda T, Tanaka H, Araoka T, Sato-Otubo A, Takahashi K, Sato Y, Yamaji N, Ogawa S, Yamanaka S, Osafune K. Cell therapy using hiPSC-derived renal progenitors ameliorates acute kidney injury in mice. Stem Cells Transl Med. 2015 Sep;4(9):980-92. doi: 10.5966/sctm.2014-0219.

 

Miki K, Endo K, Takahashi S, Funakoshi S, Takei I, Katayama S, Toyoda T, Kotaka M, Takaki T, Umeda M, Okubo C, Nishikawa M, Oishi A, Narita M, Miyashita I, Asano K, Hayashi K, Osafune K, Yamanaka S, Saito H, Yoshida Y. Efficient Detection and Purification of Cell Populations Using Synthetic MicroRNA Switches. Cell Stem Cell. 2015 Jun 4;16(6):699-711. doi: 10.1016/j.stem.2015.04.005.

 

Toyoda T, Mae S, Tanaka H, Kondo Y, Funato M, Hosokawa Y, Sudo T, Kawaguchi Y, Osafune K. Cell aggregation optimizes the differentiation of human ESCs and iPSCs into pancreatic bud-like progenitor cells. Stem Cell Res. 2015 Mar;14(2):185-97. doi: 10.1016/j.scr.2015.01.007.

 

Gotoh S, Ito I, Nagasaki T, Yamamoto Y, Konishi S, Korogi Y, Matsumoto H, Muro S, Hirai T, Funato M, Mae S, Toyoda T, Sato-Otsubo A, Ogawa S, Osafune K, Mishima M. Generation of alveolar epithelial spheroids via isolated progenitor cells from human pluripotent stem cells. Stem Cell Reports. 2014 Sep 9;3(3):394-403. doi: 10.1016/j.stemcr.2014.07.005.

 

An D, Lessard SJ, Toyoda T, Lee MY, Koh HJ, Qi L, Hirshman MF, Goodyear LJ. Overexpression of TRB3 in muscle alters muscle fiber type and improves exercise capacity in mice. Am J Physiol Regul Integr Comp Physiol. 2014 Jun 15;306(12):R925-33. doi: 10.1152/ajpregu.00027.2014. Epub 2014 Apr 16.

 

Nakagawa M, Taniguchi Y, Senda S, Takizawa N, Ichisaka T, Asano K, Morizane A, Doi D, Takahashi J, Nishizawa M, Yoshida Y, Toyoda T, Osafune K, Sekiguchi K, Yamanaka S. A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells. Sci Rep. 2014 Jan 8;4:3594. doi: 10.1038/srep03594.

 

Mae S, Shono A, Shiota F, Yasuno T, Kajiwara M, Gotoda-Nishimura N, Arai S, Sato-Otubo A, Toyoda T, Takahashi K, Nakayama N, Cowan CA, Aoi T, Ogawa S, McMahon AP, Yamanaka S, Osafune K. Monitoring and robust induction of nephrogenic intermediate mesoderm from human pluripotent stem cells. Nat Commun. 2013;4:1367. doi: 10.1038/ncomms2378.

 

Koh HJ, Toyoda T, Didesch MM, Lee MY, Sleeman MW, Kulkarni RN, Musi N, Hirshman MF, Goodyear LJ. Tribbles 3 mediates endoplasmic reticulum stress-induced insulin resistance in skeletal muscle. Nat Commun. 2013;4:1871. doi: 10.1038/ncomms2851.

 

Toyoda T, Egawa T, Hayashi T. Metabolic Sensor for Low Intensity Exercise: Insights from AMPKα1 Activation in Skeletal Muscle. J Phys Fitness Sports Med. 2012 May;1(1):59-64

 

Toyoda T, An D, Witczak CA, Koh HJ, Hirshman MF, Fujii N, Goodyear LJ. Myo1c regulates glucose uptake in mouse skeletal muscle. J Biol Chem. 2011 Feb 11;286(6):4133-40. doi: 10.1074/jbc.M110.174938. Epub 2010 Dec 2.

 

Koh HJ, Toyoda T, Fujii N, Jung MM, Rathod A, Middelbeek RJ, Lessard SJ, Treebak JT, Tsuchihara K, Esumi H, Richter EA, Wojtaszewski JF, Hirshman MF, Goodyear LJ. Sucrose nonfermenting AMPK-related kinase (SNARK) mediates contraction-stimulated glucose transport in mouse skeletal muscle. Proc Natl Acad Sci U S A. 2010 Aug 31;107(35):15541-6. doi: 10.1073/pnas.1008131107. Epub 2010 Aug 16.

 

Vichaiwong K, Purohit S, An D, Toyoda T, Jessen N, Hirshman MF, Goodyear LJ. Contraction regulates site-specific phosphorylation of TBC1D1 in skeletal muscle. Biochem J. 2010 Oct 15;431(2):311-20. doi: 10.1042/BJ20101100.

 

Lauritzen HP, Galbo H, Toyoda T, Goodyear LJ. Kinetics of contraction-induced GLUT4 translocation in skeletal muscle fibers from living mice. Diabetes. 2010 Sep;59(9):2134-44. doi: 10.2337/db10-0233. Epub 2010 Jul 9.

 

An D, Toyoda T, Taylor EB, Yu H, Fujii N, Hirshman MF, Goodyear LJ. TBC1D1 regulates insulin- and contraction-induced glucose transport in mouse skeletal muscle. Diabetes. 2010 Jun;59(6):1358-65. doi: 10.2337/db09-1266. Epub 2010 Mar 18.

 

Witczak CA, Jessen N, Warro DM, Toyoda T, Fujii N, Anderson ME, Hirshman MF, Goodyear LJ. CaMKII regulates contraction- but not insulin-induced glucose uptake in mouse skeletal muscle. Am J Physiol Endocrinol Metab. 2010 Jun;298(6):E1150-60. doi: 10.1152/ajpendo.00659.2009. Epub 2010 Mar 9.

 

Treebak JT, Taylor EB, Witczak CA, An D, Toyoda T, Koh HJ, Xie J, Feener EP, Wojtaszewski JF, Hirshman MF, Goodyear LJ. Identification of a novel phosphorylation site on TBC1D4 regulated by AMP-activated protein kinase in skeletal muscle. Am J Physiol Cell Physiol. 2010 Feb;298(2):C377-85. doi: 10.1152/ajpcell.00297.2009. Epub 2009 Nov 18.

 

Gollisch KS, Brandauer J, Jessen N, Toyoda T, Nayer A, Hirshman MF, Goodyear LJ. Effects of exercise training on subcutaneous and visceral adipose tissue in normal- and high-fat diet-fed rats. Am J Physiol Endocrinol Metab. 2009 Aug;297(2):E495-504. doi: 10.1152/ajpendo.90424.2008. Epub 2009 Jun 2.

 

Ma X, Iwanaka N, Masuda S, Karaike K, Egawa T, Hamada T, Toyoda T, Miyamoto L, Nakao K, Hayashi T. Morus alba leaf extract stimulates 5'-AMP-activated protein kinase in isolated rat skeletal muscle. J Ethnopharmacol. 2009 Feb 25;122(1):54-9. doi: 10.1016/j.jep.2008.11.022. Epub 2008 Dec 3.

 

Fujii N, Ho RC, Manabe Y, Jessen N, Toyoda T, Holland WL, Summers SA, Hirshman MF, Goodyear LJ. Ablation of AMP-activated protein kinase alpha2 activity exacerbates insulin resistance induced by high-fat feeding of mice. Diabetes. 2008 Nov;57(11):2958-66. doi: 10.2337/db07-1187. Epub 2008 Aug 26.

 

Tanaka S, Hayashi T, Toyoda T, Hamada T, Shimizu Y, Hirata M, Ebihara K, Masuzaki H, Hosoda K, Fushiki T, Nakao K. High-fat diet impairs the effects of a single bout of endurance exercise on glucose transport and insulin sensitivity in rat skeletal muscle. Metabolism. 2007 Dec;56(12):1719-28.

 

Miyamoto L, Toyoda T, Hayashi T, Yonemitsu S, Nakano M, Tanaka S, Ebihara K, Masuzaki H, Hosoda K, Ogawa Y, Inoue G, Fushiki T, Nakao K. Effect of acute activation of 5'-AMP-activated protein kinase on glycogen regulation in isolated rat skeletal muscle. J Appl Physiol. 2007 Mar;102(3):1007-13. Epub 2006 Nov 22.

 

Nakano M, Hamada T, Hayashi T, Yonemitsu S, Miyamoto L, Toyoda T, Tanaka S, Masuzaki H, Ebihara K, Ogawa Y, Hosoda K, Inoue G, Yoshimasa Y, Otaka A, Fushiki T, Nakao K. α2 isoform-specific activation of 5'adenosine monophosphate-activated protein kinase by 5-aminoimidazole-4-carboxamide-1-β-D-ribonucleoside at a physiological level activates glucose transport and increases glucose transporter 4 in mouse skeletal muscle. Metabolism. 2006 Mar;55(3):300-8.

 

Toyoda T, Tanaka S, Ebihara K, Masuzaki H, Hosoda K, Sato K, Fushiki T, Nakao K, Hayashi T. Low-intensity contraction activates the alpha1-isoform of 5'-AMP-activated protein kinase in rat skeletal muscle. Am J Physiol Endocrinol Metab. 2006 Mar;290(3):E583-90. Epub 2005 Oct 25.

 

Tanaka T, Hidaka S, Masuzaki H, Yasue S, Minokoshi Y, Ebihara K, Chusho H, Ogawa Y, Toyoda T, Sato K, Miyanaga F, Fujimoto M, Tomita T, Kusakabe T, Kobayashi N, Tanioka H, Hayashi T, Hosoda K, Yoshimatsu H, Sakata T, Nakao K. Skeletal muscle AMP-activated protein kinase phosphorylation parallels metabolic phenotype in leptin transgenic mice under dietary modification. Diabetes. 2005 Aug;54(8):2365-74.

 

Toyoda T, Hayashi T, Miyamoto L, Yonemitsu S, Nakano M, Tanaka S, Ebihara K, Masuzaki H, Hosoda K, Inoue G, Otaka A, Sato K, Fushiki T, Nakao K. Possible involvement of the alpha1 isoform of 5'AMP-activated protein kinase in oxidative stress-stimulated glucose transport in skeletal muscle. Am J Physiol Endocrinol Metab. 2004 Jul;287(1):E166-73. Epub 2004 Mar 16.

 

Manabe Y, Toyoda T, Kuroda K, Imaizumi M, Yamamoto T, Fushiki T. Effect of diazepam binding inhibitor (DBI) on the fluid intake, preference and the taste reactivity in mice. Behav Brain Res. 2001 Nov 29;126(1-2):197-204.