Machida A, Kondo K, Wakabayashi KI, Tanaka K, Hisabori T.
Molecular bulkiness of a single amino acid in the F1 α subunit determines the robustness of cyanobacterial ATP synthase.
Plant Cell Physiol. 2023 Sep 14:pcad109. doi: 10.1093/pcp/pcad109
Akiyama K, Ozawa SI, Takahashi Y, Yoshida K, Suzuki T, Kondo K, Wakabayashi KI, Hisabori T.
Two specific domains of the γ subunit of chloroplast FoF1 provide redox regulation of the ATP synthesis through conformational changes
Proc Natl Acad Sci U S A. 2023 Feb 7;120(6):e2218187120. doi: 10.1073/pnas.2218187120.
Sekiguchi T, Yoshida K, Wakabayashi KI, Hisabori T.
Dissipation of the proton electrochemical gradient in chloroplasts promotes the oxidation of ATP synthase by thioredoxin-like proteins
J Biol Chem. 2022 Sep 27;298(11):102541. doi: 10.1016/j.jbc.2022.102541
Kondo K, Izumi M, Inabe K, Yoshida K, Imashimizu M, Suzuki T, Hisabori T.
The phototroph-specific β-hairpin structure of the γ subunit of F o F 1-ATP synthase is important for efficient ATP synthesis of cyanobacteria
J Biol Chem. 2021 Jul 31;297(3):101027.doi: 10.1016/j.jbc.2021.101027
Sekiguchi T, Yoshida K, Okegawa Y, Motohashi K, Wakabayashi KI, Hisabori T.
Chloroplast ATP synthase is reduced by both f-type and m-type thioredoxins.
Biochim Biophys Acta Bioenerg. 2020 Jul 11;1861(11):148261. doi: 10.1016/j.bbabio.2020.148261
Akiyama K, Kondo K, Inabe K, Murakami S, Wakabayashi KI, Hisabori T.
The β-hairpin region of the cyanobacterial F1-ATPase γ-subunit plays a regulatory role in the enzyme activity.
Biochem J. 2019 Jun 26;476(12):1771-1780. doi: 10.1042/BCJ20190242.
Inabe K, Kondo K, Yoshida K, Wakabayashi KI, Hisabori T.
The N-terminal region of the ? subunit from cyanobacterial ATP synthase alone can inhibit ATPase activity.
J Biol Chem. 2019 Jun 28;294(26):10094-10103. doi: 10.1074/jbc.RA118.007131.
Murakami S, Kondo K, Katayama S, Hara S, Sunamura EI, Yamashita E, Groth G, Hisabori T.
Structure of the γ-ε complex of cyanobacterial F1-ATPase reveals a suppression mechanism of the γ subunit on ATP-hydrolysis in phototrophs.
Biochem J. 2018 Sep 18;475(18):2925-2939. doi: 10.1042/BCJ20180481.
Kondo K, Takeyama Y, Sunamura EI, Madoka Y, Fukaya Y, Isu A, Hisabori T.
Amputation of a C-terminal helix of the γ subunit increases ATP-hydrolysis activity of cyanobacterial F1 ATP synthase.
Biochim Biophys Acta. 2018 May;1859(5):319-325. doi: 10.1016/j.bbabio.2018.02.004.
Buchert F, Bailleul B, Hisabori T.
A γ-subunit point mutation in Chlamydomonas reinhardtii chloroplast F1Fo-ATP synthase confers tolerance to reactive oxygen species.
Biochim Biophys Acta. 2017 Dec;1858(12):966-974. doi: 10.1016/j.bbabio.2017.09.001.
Buchert F, Konno H, Hisabori T.
Redox regulation of CF1-ATPase involves interplay between the γ-subunit neck region and the turn region of the βDELSEED-loop.
Biochim Biophys Acta. 2015 Apr-May;1847(4-5):441-50. doi:10.1016/j.bbabio.2015.01.013.
Sunamura EI, Kamei T, Konno H, Tamaoki N, Hisabori T.
Reversible control of F1-ATPase rotational motion using a photochromic ATP analog at the single molecule level.
Biochem Biophys Res Commun. 2014 Mar 28;446(1):358-63. doi: 10.1016/j.bbrc.2014.02.117. [Epub ahead of print]
Hisabori T, Sunamura EI, Kim Y, Konno H.
The Chloroplast ATP Synthase Features the Characteristic Redox Regulation Machinery.
Antioxid Redox Signal. 2013 Nov 20;19(15):1846-54. doi: 10.1089/ars.2012.5044.
Sunamura E, Konno H, Imashimizu M, Mochimaru M, Hisabori T.
A conformational change of the γ subunit indirectly regulates the activity of cyanobacterial F1-ATPase.
J Biol Chem. 2012 Nov 9;287(46):38695-704. doi: 10.1074/jbc.M112.395053. Epub 2012 Sep 25.
Konno H, Nakane T, Yoshida M, Ueoka-Nakanishi H, Hara S, Hisabori T.
Thiol modulation of the chloroplast ATP synthase is dependent on the energization of thylakoid membranes.
Plant Cell Physiol. 2012 Apr;53(4):626-34. doi: 10.1093/pcp/pcs018. Epub 2012 Feb 22.
Imashimizu M, Bernat G, Sunamura E, Broekmans M, Konno H, Isato K, Rogner M, Hisabori T.
Regulation of F0F1-ATPase from Synechocystis sp. PCC 6803 by gamma and epsilon subunits is significant for light/dark adaptation.
J Biol Chem. 2011 Jul 29;286(30):26595-602. Epub 2011 May 24.
Konno H, Isu A, Kim Y, Murakami-Fuse T, Sugano Y, Hisabori T.
Characterization of the relationship between ADP-inhibition and {epsilon}-inhibition in cyanobacterial F1-ATPase.
J Biol Chem. 2011 Apr 15;286(15):13423-9. Epub 2011 Feb 23.
Kim Y, Konno H, Sugano Y, Hisabori T.
Redox regulation of rotation of the cyanobacterial F1-ATPase containing thiol regulation switch.
J Biol Chem. 2011 Jan 4. 286(11) 9071-9078
Ei-Ichiro Sunamura, Hiroki Konno, Mari Imashimizu-Kobayashi, Yasushi Sugano, and Toru Hisabori
Physiological Impact of Intrinsic ADP-Inhibition of Cyanocbacterial FoF1 Conferred by the Inherent Inserted Sequence into the {gamma} Subunit
Plant and Cell Physiology Advance Access published on April 25, 2010. doi:10.1093/pcp/pcq061
Yagi H, Konno H, Murakami-Fuse T, Isu A, Oroguchi T, Akutsu H, Ikeguchi M, Hisabori T.
Structural and functional analysis of the intrinsic inhibitor subunit epsilon of F1-ATPase from photosynthetic organisms.
Biochem J. 2009 Dec 14;425(1):85-94.
Meiss E, Konno H, Groth G, Hisabori T.
Molecular processes of inhibition and stimulation of ATP synthase caused by the phytotoxin tentoxin.
J Biol Chem. 2008 Sep 5;283(36):24594-9. Epub 2008 Jun 25
Konno H, Murakami, T, Fujii F, Koyama F, Ueoka-Nakanishi H, Pack C-G, Kinjo M, Hisabori T.
The regulator of the F1 motor: Inhibition of rotation of cyanobacterial F1-ATPase by the esubunit.
EMBO Journal , 2006 25(19):4596-4604
Lill, H., Hisabori, T., Groth, G., Bald, D. (2004)
A thermostable enzyme as an experimental platform to study properties of less stable homologues
Protein Engineering, Design & Selection, ?17, 553-555
Konno, H., Suzuki, T., Bald, D., Yoshida, M., Hisabori, T. (2004)
Significance of the epsilon subunit in the thiol modulation of the chloroplast ATP synthase
Biochem. Biophys. Res. Commun. ,?318, 17-24
Ueoka-Nakanishi, H., Nakanishi, Y., Konno, H., Motohashi, K., Bald, D., Hisabori, T. (2004)
Inverse regulation of rotation of F1-ATPase by the Mutation at the Regulatory Region on the gamma Subunit of Chloroplast ATP Synthase
J. Biol. Chem. 279, 16272-16277
Pavlova, P., Shimabukuro, K., Hisabori, T., Groth, G., Lill, H. and Bald, D. (2004)
Complete inhibition and partial re-activation of single F1-ATPase molecules by tentoxin: New properties of the re-activated enzyme
J. Biol. Chem. 279, 9685-9688
Hisabori, T., Ueoka-Nakanishi, H., Konno, H., Koyama, F. ?(2003)
Molecular evolution of the modulator of the chloroplast ATP synthase: Origin of the conformational change dependent regulation
FEBS Lett. 545(1):71-75
Sugiyama, K., Hisabori, T. (2003)
Conformational change of the chloroplast ATP synthase on the enzyme activation process detected by the trypsin sensitivity of the gamma subunit
Biochem. Biophys. Res. Commun. , 301, 311-316
Hisabori, T., Konno, H., Ichimura, H., Strotmann, H. and Bald, D. (2002)
Molecular devices of chloroplast F1-ATP synthase for the regulation
Biochim. Biophys. Acta , 1555, 140-146
Groth G., Hisabori, T., Lill, H., Bald, D. (2002)
Substitution of a single amino acid switches the tentoxin resistant thermophilic F1-ATPase into a tentoxin sensitive enzyme
J. Biol. Chem. , 277, 20117-20119
Bald D, Noji H, Yoshida M, Hirono-Hara Y, Hisabori T. (2001)
Redox regulation of the rotation of F1-ATP synthase.
J. Biol. Chem. 276, 39505-39507
Konno H, Yodogawa M, Stumpp MT, Kroth P, Strotmann H, Motohashi K, Amano T, Hisabori T. (2000)
Inverse regulation of F1-ATPase activity by a mutation at the regulatory region on the gamma subunit of chloroplast ATP synthase
Biochem. J. 352, 783-788
Bald D, Noji H, Stumpp MT, Yoshida M, Hisabori T (2000)
ATPase activity of a highly stable alpha3beta3gamma subcomplex of thermophilic F1 can be regulated by the introduced regulatory region of gamma subunit of chloroplast F1
J. Biol. Chem. 275, 12757-12762
Hisabori T, Kondoh A, Yoshida M (1999)
The gamma subunit in chloroplast F1-ATPase can rotate in a unidirectional and counter-clockwise manner
FEBS Lett. 463, 35-38
Stumpp MT, Motohashi K, Hisabori T (1999)
Chloroplast thioredoxin mutants without active site cysteines facilitate the reduction of the regulatory disulfide bridge on the gamma subunit of chloroplast ATP synthase
Biochem. J. 341, 157-163
Kanekatsu M, Saito H, Motohashi K, Hisabori T. (1998)
Thebeta Subunit of Chloroplast ATP Synthase (CFoCF1-ATPase) Is Phosphorylated by Casein Kinase II
Biochem. Mol. Biol. Int. 46, 99 105
Hisabori T, Motohashi K, Kroth P, Strotmann H, Amano T (1998)
The Formation or the Reduction of a Disulfide Bridge on thegamma Subunit of Chloroplast ATP Synthase Affects the Inhibitory Effect of the epsilon Subunit.
J. Biol. Chem. 273, 15901-15905
Hisabori T, Kato Y, Motohashi K, Kroth-Pancic P, Strotmann H, Amano T. (1997)
The Regulatory Functions of thegamma and epsilon Subunits from Chloroplast CF1 Are Transferred to the Core Complex,alpha3beta3, from Thermophilic Bacterial F1.
Eur. J. Biochem. 247, 1158-1165