The thesis investigates the potential of Oxford Nanopore Sequencing (ONS) as a high-resolution analytical tool for assessing mRNA stability. The three main objectives of this project are as follows:

1. Assess the stability of naked and lipid nanoparticle (LNP)-encapsulated eGFP mRNA subjected to accelerated thermal degradation studies (25°C, 35°C, and 50°C) and analyse the samples using Capillary Electrophoresis (CE) and ONS comparing the two methods.

2. Investigate whether mRNA fragmentation occurs randomly or not using ONS data.

3. Identify the limitations of ONS as a tool for mRNA stability testing.