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UK manufacturers of quality survival gear for teams, organisations and individuals. Through innovative thinking and the high quality of UK manufacture we aim to offer the best and most effective outdoor survival products.

...when only the best will do IntroductionWith over 160 years industry experience Survitec Group remains market leader in design and manufacture of survival equipment OUR MISSION Our mission is to continually strengthen our position as global leader in the supply of survival equipment to the maritime industry through customer focus, product excellence and technical innovation SERVICE AND DISTRIBUTION The Survitec Group has a global distribution network of over 80 distributors and agents. Each of our partners has comprehensive industry experience ensuring we can provide you with the...

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Platelet thrombi and vascular inflammation are prominent features of discordant xenograft rejection. The purinergic nucleotides ATP and ADP, which are secreted from platelets and released by injured endothelial cells (EC), are important mediators of these reactions. Quiescent EC express the ectoenzyme ATP-diphosphohydrolase (ATPDase; an apyrase), which exerts an important thromboregulatory function by hydrolyzing both ATP and ADP. We have shown that ATPDase activity is rapidly lost from the surface of the EC following ischemia-reperfusion injury and during xenograft rejection. The aim of this study was to supplement ATPDase activity within xenografts by infusion of soluble apyrases, and thereby validate the importance of local ATPDase activity in the modulation of xenograft rejection. Lewis rats underwent heterotopic cardiac xenografting from guinea pigs and apyrase was administered intravenously (200 U/kg) as a single dose to evaluate effects on hyperacute rejection (HAR). This initial dose was followed by a continuous apyrase infusion (8.0 U/kg/hr) directly into the graft aorta in combination with systemic cobra venom factor (CVF) administration to deplete complement when delayed xenograft rejection (DXR) was studied. Functional apyrase levels in vivo were assessed by the capacity of blood samples taken at the time of surgery and rejection to inhibit platelet aggregation in vitro. Apyrase administration significantly prolonged graft survival in HAR and DXR. Functional assays showed inhibition of platelet aggregation suggesting effective systemic antiaggregatory effects of the administered apyrases. Histologic studies showed that apyrase administration abrogated local platelet aggregation and activation in HAR and DXR. Our data demonstrate that local administration of apyrase prolonged discordant xenograft survival. These observations emphasize the potential importance of purinergic mediators in platelet activation during xenograft rejection.

The western basin of Lake Erie receives input from two main tributaries (Maumee and Detroit River), which differ greatly in their nutrient and sediment loading. The higher turbidity of the Maumee River plume is thought to reduce predation on early-stage juvenile yellow perch (Perca flavescens), consequently increasing their survival. For this reason, my overall objective was to evaluate the effect of the Maumee River plume on the overall recruitment of larval yellow perch to the juvenile stage. However, traditional diet analyses are not effective for evaluating larval predation rates. I therefore review genetic and non-genetic diet analysis techniques, and how they have evolved with technological advances, allowing researchers to effectively explore trophic interactions and energy movement in aquatic ecosystems. This provided a framework for my doctoral research for which I used a variety of molecular genetic techniques to estimate survival of larval yellow perch using population genetics and predation rates through stomach content analysis of predator fish. Using yellow perch microsatellite markers, I measured temporal and spatial genetic structure in larval yellow perch, while Bayesian genotype assignment provided relative larval survival estimates for yellow perch inhabiting the Maumee and Detroit River plumes. Overall, genotype assignment of Age-0 yellow perch establishes that, in the western basin of Lake Erie, larval recruitment to the juvenile stage is significantly higher for fish inhabiting the Maumee River plume relative to those in the Detroit River plume. In addition, I utilized molecular genetic techniques to accurately identify highly digested early-stage juvenile prey to the species level which was not possible with a more traditional approach (visual inspection of gut contents). Specifically, I use polymerase chain reaction (PCR), cloning and sequencing, to demonstrate the diversity of prey consumed by several freshwater fish species. Finally, using species-specific single nucleotide polymorphism (SNP) assays and microsatellite markers, I quantified predation and cannibalism experienced by larval yellow perch in the Maumee and Detroit river plumes. The combination of markers showed generally higher predation and cannibalism in the less turbid waters of the Detroit River plume, indicative of river-plume effects (possibly mediated through turbidity) reducing larval yellow perch predation mortality. My doctoral research shows the likely mechanism that river plumes in the western basin of Lake Erie contribute to larval yellow perch survival and consequently potential recruitment; however, temporal and spatial variability indicate that other factors also contribute.

Carreon Martinez, Lucia Beatriz, "River plume effects on larval yellow perch (Perca flavescens ) survival and predation in the western basin of Lake Erie" (2012). Electronic Theses and Dissertations. 5588.