Daping Fan, PhD, University of South Carolina
PROJECT TITLE: Microarray analysis of macrophages with TFEB manipulation
Breast cancer is the most prevalent cancer and the second leading cause of cancer-related death in women in the United States. Tumor-associated macrophages (TAMs) play critical role in breast cancer development. Understanding how the functions of TAMs are regulated will provide new strategy for preventing and treating breast cancer.
The tumor microenvironment (TME) is comprised of a variety of infiltrating immune cells. Those cells play pivotal roles in tumor initiation, progression and metastasis. Among them, tumor-associated macrophages (TAMs) and their alternative activation contribute greatly to the progression of tumors. The mechanisms governing macrophage polarization in the TME are unclear. This project was designed to utilize the resources of the Microarray Core Facility at the University of South Carolina in order to provide a more detailed understanding of macrophage function in breast cancer.
A microarray has been performed to examine how TFEB overexpression affects gene expression profile in macrophages treated with breast tumor conditioned medium. The results will guide further investigation to understand the regulation of macrophage polarization in breast cancer.
A SC INBRE Bioinformatics Pilot Project Program grant was awarded to perform a microarray study to examine how TFEB overexpression influences macrophage polarization in the context of breast cancer.
The study supported by the pilot project grant provided us with the necessary information regarding the effect of TFEB manipulation on TAM polarization in the TME for us to more effectively determine how to pursue strategies for activating the antitumor immune response in the immunosuppressive neoplastic microenvironment.
January 19, 2018