Science Research Portfolio
Samantha Sanchez
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Science Research Journey
Science Research Journey
My passion for medicine started when I was very young, and cancer affected someone I love. Cancer was a topic that always intrigued me but that I never thought it would become my current research. Thanks to the help of Professor Melanie Rutkowski, at the University of Viginia I was able to understand ovarian cancer and to successfully complete my project.
Science Research - Third Year
Science Research - Third Year
Research Paper
Research Paper
Abstract: This research provides important insights into how TLR5 signaling affects the tumor microenvironment in ovarian cancer. The overall purpose of the research was to investigate whether TLR5 signaling contributes to a more suppressive environment that could hinder the effectiveness and responses of the tumor microenvironment. The hypothesis that TLR5 signaling makes myeloid cells more suppressive in the ovarian tumor microenvironment was partially supported by the findings. Specifically, as a result of the methodology it was shown that Arginase 1 had a greater expression in macrophages. This information is relevant because other markers used in the course of the investigation, such as IL-6, IL-10, IL-12 and TNF, did not show significant changes.The methodology involved in this research involved the use of Flowjo software to analyze twenty-five samples of TLR5 inhibition. These samples were first divided into two groups, the first group that shed beads and the second non beads region. These samples were analyzed with five different markers that consisted of ZA-LD, CD45, CD3+CD19, CD11B, and F480. This investigation additionally aimed to provide further insights into the role of TLR5 signaling and its impact on myeloid cells. The results of this investigation suggest that TLR5 signaling indeed promotes a suppressive phenotype in the tumor microenvironment, by increasing the expression of arginine 1 and macrophages. These findings have important implications in the development of new therapies for cancer. In the future, targeting TLR 5 can be a potential new strategy to reduce the suppressive activity in the tumor microenvironment and in this way increasing the effectiveness of immune therapies.
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