Now that we have explored the quality of our raw reads, we can move on to read alignment. We perform read alignment or mapping to determine where in the genome the reads originated from. The alignment process consists of choosing an appropriate reference genome to map our reads against and performing the read alignment using one of several splice-aware alignment tools such as STAR or HISAT2. The choice of aligner is often a personal preference and also dependent on the computational resources that are available to you.

To determine where on the human genome our reads originated from, we will align our reads to the reference genome using STAR (Spliced Transcripts Alignment to a Reference). STAR is an aligner designed to specifically address many of the challenges of RNA-seq data mapping using a strategy to account for spliced alignments.


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For every read that STAR aligns, STAR will search for the longest sequence that exactly matches one or more locations on the reference genome. These longest matching sequences are called the Maximal Mappable Prefixes (MMPs):

This sequential searching of only the unmapped portions of reads underlies the efficiency of the STAR algorithm. STAR uses an uncompressed suffix array (SA) to efficiently search for the MMPs, this allows for quick searching against even the largest reference genomes. Other slower aligners use algorithms that often search for the entire read sequence before splitting reads and performing iterative rounds of mapping.

Believers also say that starseeds have the desire to explore and experience new cultures and spheres, which help star people to then provide novel insights into existence. Examples include new (conspiracy) theories about society, holistic health interventions along with thoughts on ancient aliens and civilisations.

Certain personality characteristics may also incline some people to believe in the notion of starseeds. For instance, if you are fantasy prone and often confuse imaginary and real events you may see the theory of alien consciousness as profound and desirable.

In the case of starseeds, several books published by big publishing houses may provide a sense of authenticity and so too does the fact that a number of them are bestsellers. Indeed, it seems life as we know it, may not be as straightforward as we once imagined.

By the way, I love that each volume can kind of be read on its own as a whole, but they echo and build on each other too. How would you characterize this new volume as compared to the other two? To me, it almost feels like a biblical action comic, but how would you describe the new one?

And I think that it keeps going back to this idea that Joseph Campbell reiterates in his talks: about how for a myth to be alive, to experience it, it has to be transparent. So it's like it's open. It's open to time. It's open to be reinterpreted. It's open to be, you know, to be reread and be seen differently from the first time you've read it. And I think that's why I chose more of this poetic, metaphorical language, utilizing concepts, trying to-- I guess trying to make something that's poetic using scientific concepts. So if you understand the concepts that are in the language, then it becomes very poetic. It becomes very metaphorical. And I think one of the things is, it's definitely a book for somebody who's initiated. For those who are not, I'm sorry. I realize that I should have done something that was like taking somebody by their hand and introducing this world to them. But I think at the end I can only make work that's in my own language. So I hope maybe sooner or later there will be a reader.

And then I figured out how just magnificent his art was, how he could make my eye move through the page, how he could manipulate the reader's eye to action and direction and high drama. And his Kirby Tech has always amazed me. Every time I see Kirby Tech, I am in awe of what it is and what it could mean, because it's abstract art. I look at it as abstract art. And anytime I have an opportunity to talk about anything that's biological or technological or biotech, I think of Kirby Tech. I think about that way of communicating the idea of circuits and things connected and things pulsating and moving. It's organic, but at the same time it's technological. I'm in awe of it. I think it should be seen as high art.

I have the whole Captain Victory series, and I've never read it. I bought it off eBay... I have the whole series. But I haven't sat down and read it. I've read Silver Star, and I have the Image Comics hardcover. It's not one of Kirby's greatest works, but it has some really good parts in it and some really great ideas.

But at the same time, I like the grid because the Starseeds story is pretty wild. So I always think I need to have a base for the storytelling for readers, so they don't get lost. So I keep going back to the grid - even though I break it, I keep going back to it because it's easier to read, or it's easier for me to tell the story than going off into something that's really very wild idea-wise, content-wise, and then making very wild page designs. Even though I think that's where it should move, I still am not confident enough that I can pull it off. So I tend to go back to the grids and play between the grids and their variations.

And I think that's happening because we've lost our rebellion stories to somebody else telling us the narrative. I don't know how we get there, but to be able to tell our stories, our individual stories, not as a group or as a community, but as individuals to say-- to rise up and say, you know, fuck that. Fuck the government. Fuck the churches. Fuck the economy. Fuck the social and cultural structures. Those stories are fucking archaic now. They don't seem to help humanity to grow into something else. They're just pushing us into the hole we are already in. So to me, it's kind of like, what if we change the story? What if we take the opportunity to say the story we've told as humanity for the past, I don't know, 10,000 years, is one fucked-up story? Do you choose to live with that, and keep repeating that story over and over again expecting a different result? It's not going to work - so try to figure out different stories. And I think presently we see the younger generation has different ways of telling the stories of how they see the world. And I'm like, they have it right there. That's the change. That's how it should be. That's how we should let the younger generation change the present.

The last thing I could say is that it's a book-- from what I've read and heard from the people, it's a book that you experience. It's not giving you or telling you what the meaning is that you're reading. It's up to the reader to want to sit down and contemplate what you've read. And that makes it into, I guess from what I've heard, a hard read, if you're not into that. But I think if you give yourself the time to read and to contemplate what you've read, you'll be elated, and it will give you some joy. And maybe that joy is not because you've read the book, but it's more because you took time to contemplate things you already knew, that you haven't really taken the time to think about.

& then there are Crystal children, Rainbow children & Indigo children who are truly from everywhere. & our Venusians who are from the planet of LOVE, and our Mintakans who are so powerful yet tend to feel really lost in earlier years of life because their planet no longer exists. Not to mention our Lemurian & Atlantean mermaid humans, connected to the water, the mountains, Mt. Shasta & realms beyond. AND our Lyran feline goddesses, tuned into divine feminine ancient wisdom of the ages. And there are so many others, as well. Here you will read about all of them!

Unless you have already been through this and come out on the other side, you likely struggle with not knowing what your true purpose is. This can be especially hard because of your sensitive makeup and your ability to see all sides of the coin.

Some of your gifts include being highly intuitive and able to read energy easily. You are a natural healer and are able to influence the thoughts and emotions of others. A wonderful endeavor for you would be to pursue leading a heart-based project you are drawn to.

Light Workers are very special starseed souls that come from a variety of different realms, planets, and star systems. There are over 25 known types of lightworkers! On Earth, the Light Worker purpose is to help humanity and other worlds evolve through spreading love, light, and kindness. As a Light Worker, you are here solely to work for all things light.

Do you have a cat? If not, it might be nice to get one or form connections with someone in your life who has a cat. This healing feline energy is very potent for Lyrans. Unlike many other starseeds, Lyrans a.k.a Feline starseeds feel very at home on Earth. They love the chaotic nature of Earth life and the undeniable freedom that comes with it.

Stars are not spread uniformly across the universe but are normally grouped into galaxies along with interstellar gas and dust. A typical large galaxy like the Milky Way contains hundreds of billions of stars. There are more than 2 trillion (1012) galaxies, though most are less than 10% the mass of the Milky Way.[104] Overall, there are likely to be between 1022 and 1024 stars[105][106] (more stars than all the grains of sand on planet Earth).[107][108][109] Most stars are within galaxies, but between 10 and 50% of the starlight in large galaxy clusters may come from stars outside of any galaxy.[110][111][112]

Due to the relatively vast distances between stars outside the galactic nucleus, collisions between stars are thought to be rare. In denser regions such as the core of globular clusters or the galactic center, collisions can be more common.[122] Such collisions can produce what are known as blue stragglers. These abnormal stars have a higher surface temperature and thus are bluer than stars at the main sequence turnoff in the cluster to which they belong; in standard stellar evolution, blue stragglers would already have evolved off the main sequence and thus would not be seen in the cluster.[123] be457b7860

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