Pi Software Suite C-990.cd1 Download

The raw sequence data were assembled and analyzed using DNA sequence analysis software (Lasergene software suite V6.0, DNASTAR). The full-length or partial HBV DNA sequences were aligned using CLUSTAL W software (version 1.83; DDBJ) along with HBV A-G genotype reference sequences retrieved from GenBank/EMBL/DDBJ. Genetic distances were estimated by Kimura's 2-parameter method, and phylogenetic trees were constructed by the neighbor-joining method using MEGA software V4 [21]. Bootstrap resampling and reconstruction with 1000 replicates were carried out to confirm the reliability of the phylogenetic trees. Intergenotypic recombination of the 19 full genomes HBV C/D recombinants were searched for with software SimPlot V3.5.1 [22].

The printed circuit boards (PCBs) have been designed using the cross-platform open-source electronic design automation suite KiCAD [24]. All design files are available online [25] or on request. The PCBs have been manufactured by the community printed circuit board service OSH Park [26] using the standard manufacturing parameters: two-layered FR4, 1.6 mm thickness, electroless nickel immersion gold finish, clearance >160 tag_hash_107m, trace width >160 tag_hash_108m, >254 tag_hash_109m drill size. The circuit board however is rather simple (e.g. stray capacitances can be largely neglected) and a DIY solution using presensitized PCBs, UV exposure, fixation and etchants like iron(III) chloride or hydrogen peroxide/hydrochloric acid give very good results. An entire assembly using perfboard likely requires wired components, instead of the surface-mounted devices (SMD).