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A graphene oxide-based tool-kit capable of characterizing and classifying exonuclease activities
Author : Song, J., Hoa, V. M., Yoo, J., Oh, S., Im, H., Park, D., & Lee, G.
Source RSC Advances 7: 14917-14922
Year 2017
ABSTRACT
Enzyme kinetics and classification are key topics in modern biochemistry. Here, we have developed a simple, rapid, and quantitative assay via a terminally capped DNA probe, reaction-specific-labeling and the fluorescence-quenching capability of graphene-oxide. Our technique provides not only characterization of kinetic information but also the immediate classification of an exonuclease without needing to design a set of DNA substrates.
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