Seven years after the events of the first film, Johnny English has been training in Tibet to move past the shame of a failed mission to protect the newly elected president in Mozambique, which cost him his knighthood. Called back into service by MI7 under his new boss Pamela Thornton, codenamed "Pegasus", English is sent to investigate a plot to assassinate the Chinese Premier during talks with the Prime Minister. He meets fellow agent and old acquaintance Simon Ambrose, MI7's quartermaster Patch Quartermain, and junior agent Colin Tucker, English's new assistant.
In Hong Kong, English finds former CIA agent Titus Fisher, who reveals himself to be a member of Vortex, the group responsible for sabotaging English's Mozambique mission. He reveals Vortex holds a secret weapon unlocked by three keys, held by himself and two other former spies. Fisher is killed by an elderly assassin disguised as a maid, and an accomplice steals his key. English chases the thief and recovers the key, but is duped by another Vortex operative disguised as a flight attendant en route to London, and is humiliated in a meeting with the Foreign Secretary and Pegasus when he attempts to present the key and the conspiracy.
Kate Sumner, MI7's behavioral psychologist, uses hypnosis to help English recall his suppressed memory of the Mozambique incident, revealing another Vortex operative, Russian spy Artem Karlenko, is masquerading as millionaire Sergei Pudovkin. English and Tucker meet Karlenko at a golf course outside London, but the Killer Cleaner critically injures Karlenko. English and Tucker bring him to a hospital by helicopter, but Karlenko dies after revealing the third key is held by a mole in MI7.
Over dinner, English confides with Ambrose about the mole, and Ambrose tells English that he suspects Quartermain. Tucker confronts Ambrose about him being the traitor, but English naively dismisses Tucker and lets Ambrose go with Karlenko's key. English confronts Quartermain, but realizes Ambrose has framed him as the third Vortex member. Chased by MI7 agents due to Ambrose telling Pegasus that the mole is English, he manages to escape to Sumner's flat.
Reviewing footage of the Mozambique mission, Sumner realizes the assassin was manipulated by a mind control drug, Timoxeline Barbebutenol. Ambrose comes to pick up Sumner, and English realizes he is the mole. Evading the Killer Cleaner by jumping into a garbage chute, English apologizes to Tucker and persuades him to join in infiltrating Le Bastion, a fortress in the Swiss Alps where the talks are to be held.
At the fortress, English accidentally activates a distress beacon, alerting the guards to their presence. English commands Tucker to knock him out and put him inside a body bag so they will be taken into the fortress. Inside, English manages to escape the body bag and warns Pegasus of the threat, but accidentally consumes a drink spiked with the drug, and subdues Pegasus on Ambrose's command.
Assigning English as the Prime Minister's bodyguard in place of Pegasus, Ambrose orders him to assassinate the Chinese Premier using a pistol disguised as lipstick, initially designed for Pegasus; English tries to resist the drug. Tucker interrupts Ambrose's communication feed with music before Ambrose resets it, exposing himself as the traitor. English resists again and shoots Ambrose, who escapes while the drug enters its lethal stage and English loses consciousness. Sumner arrives and revives English with a passionate kiss. English pursues Ambrose down the mountainside, and they engage in a fistfight in a cable car. English overpowers Ambrose, but falls out of the carriage. Ambrose shoots at English, who tries to use his spy umbrella as a bulletproof shield, which is revealed to be a missile launcher when he closes it. The missile destroys the carriage, killing Ambrose.
Vortex is shut down and English has his knighthood reinstated by the Queen. During the ceremony, the Killer Cleaner, disguised as the Queen, attacks English with a sword before fleeing, leading English to attack the real Queen. He only realises his mistake when the assassin is caught by the royal guards.
During the credits, English prepares dinner for Sumner to the tune of "In the Hall of the Mountain King".
Rowan Atkinson as Johnny English, an accident prone but good hearted MI7 agent.
Rosamund Pike as Kate Sumner, a behavioural psychologist at MI7, and English's love interest.
Daniel Kaluuya as Colin Tucker, an MI7 agent who becomes English's assistant and sidekick in his mission.
Dominic West as Simon Ambrose, an MI7 agent and colleague of English; later revealed as the main antagonist, as a member of Vortex.
Gillian Anderson as Pamela Thornton aka Pegasus, head of MI7.
Richard Schiff as Titus Fisher, an ex CIA operative and member of Vortex.
Tim McInnerny as Patch Quartermain, MI7's wheelchair user quartermaster.
Pik-Sen Lim as The Killer Cleaner, an assassin hired by Vortex who attempts to kill English.
Stephen Campbell Moore as the British Prime Minister.
Burn Gorman as Slater, an MI7 intelligence expert who works with Ambrose and is a member of Vortex.
Lobo Chan as Premier Xiang Ping, Chinese Premier who has a pro west stance.
Togo Igawa as Ting Wang, English's mentor in Tibet, and MI7 sleeper agent.
Benedict Wong as Chi Han Ly a Chinese official conspiring with Simon to take out Xiang Ping
Williams Belle as Ling, a Vietnamese henchman working for Vortex, who works with the Killer Cleaner.
Paul Che as Chinese Man In Spectacles 1
Courtney Wu as Chinese Man In Spectacles 2
Mark Ivanir as Artem Karlenko, a Russian (KGB) former double agent and member of Vortex.
Joséphine de La Baume as Madeleine, a deceivingly charming member of Vortex, responsible for luring English off his post in Mozambique.
Lily Atkinson, the daughter of Rowan Atkinson, made her cameo appearance as the girl that gets her helmet stolen by Johnny English. Ben Miller reprised his role of Angus Bough from the previous film in one scene, but was cut from the final film. Charles Dance and Michael Gambon made cameos as retired agents. Rupert Vansittart appears as a rich yacht owner. Miles Jupp cameos as a technician in Patch's lab.
sequel to Johnny English (2003) and the second instalment in the Johnny English series,