Erik Stifler, Steve and Matt Stifler's cousin, fails to live up to the family name as he is about to graduate from high school as a virgin. Erik feigns an illness so that he can stay home and masturbate. His parents and grandmother unexpectedly walk into the door and are hit with Erik's semen, causing his grandmother to die of a heart attack. Erik's girlfriend of two years, Tracy, loves him but is not ready for sex.
Erik's friends Cooze and Ryan plan a road trip to visit Erik's cousin Dwight Stifler at the University of Michigan for an event known as the Naked Mile. Tracy sees this as an opportunity to give Erik a sex pass, hoping that he can quench his lustful desires.
As soon as Erik and his friends arrive on campus, they witness a drinking contest where Dwight is crowned champion. Later, they lose a rough game of football against a bitter rival fraternity composed almost entirely of midgets. Later at a bar, Erik meets a college girl named Brandi who has a fetish for virgins, prompting Ryan and Cooze to make a bet regarding whether or not Erik will sleep with her.
The following morning, Dwight gets attacked by the midget fraternity while walking down the street, landing him in the hospital. However, he manages to make it to the Naked Mile where he joins up with Erik, Ryan, and Cooze; who are at first reluctant to run, but when Brandi, Jill, and Alexis strip down completely stark naked, they are finally prompted to strip down as well. Finally as Erik and Brandi reach the finish line, they share a kiss which is caught on camera for a TV news report. Watching the report about the Naked Mile at home, Tracy is upset and feels guilty that she allowed Erik the pass. Her friends convince her to also lose her virginity before he gets back.
Later that evening, Erik realizes that he loves Tracy, confesses to Brandi that he cannot sleep with her, and rushes back to see his girlfriend. When he gets to her house, Tracy's dad says she is at a party. Erik rides her horse and shows up at the party, pounds on the closed bedroom door and proclaims his love for her. However, Tracy is not in the room because she had decided that she could not go through with her plans. The two reconcile and they make love.
When Erik returns to the Beta house to pick up his friends the next morning, each guy shares stories of his sexual experiences from the night before. The guys then ask Erik if he "sealed the deal" with Brandi that night, and Erik tells them no, prompting Ryan to pay up on the bet to Cooze. Erik tells them about his adventure back home to make up with Tracy and finally lose his virginity. The guys are proud of Erik for officially living up to the Stifler family name, and the three friends go home.
During the post-Naked Mile party, Dwight spots Vicky, the girlfriend of Rock, the leader of the midget fraternity, and the two of them head up to Dwight's room to have sex. Later, Dwight sends a DVD to Rock that reads, "Payback's a bitch." It reveals both Dwight and Vicky having sex much to Rock's dismay.
John White as Erik Stifler
Steve Talley as Dwight Stifler
Jessy Schram as Tracy Sterling
Ross Thomas as Ryan Grimm
Jake Siegel as Mike "Cooze" Coozeman
Christopher McDonald as Harry Stifler
Eugene Levy as Noah Levenstein
Jordan Prentice as Rock
Maria Ricossa as Mrs. Stifler
Candace Kroslak as Brandy
Mika Winkler as Vicky
Dan Petronijevic as Bull
Jaclyn A. Smith as Jill
Angelique Lewis as Alexis
Jordan Madley as Brooke
Melanie Merkosky as Natalie
Jon Cor as Trent
Alyssa Nicole Pallett as Porn Chick
Jessica Booker as Grandma Stifler
Stuart Clow as Mr. Sterling
Joe Bostwick as Mr. Williams
Daniel Morgret as Frankie
It is the second installment in American Pie Presents film series
a spin-off of the American Pie franchise