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Background: Endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) is the standard in the diagnosis of solid pancreatic lesions, in particular when combined with rapid onsite evaluation of cytopathology (ROSE). More recently, a fork-tip needle for core biopsy (FNB) has been shown to be associated with excellent diagnostic yield. EUS-FNB alone has however not been compared with EUS-FNA + ROSE in a large clinical trial. Our aim was to compare EUS-FNB alone to EUS-FNA + ROSE in solid pancreatic lesions.

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Dell Wyse Converter for PCs is a stand-alone software that enables you to provide a thin client like experience on a Windows PC. Dell Wyse Converter for PCs provides a secure, and seamless interface to connect to Virtual Desktop Infrastructure (VDI) sessions.

Ok. Windows based terminals pose their own challenges with what you want to do. Which version of WDM are you using? If you are using the latest WDM with those models, do you have the latest firmware image and latest HAgent loaded? If not, then it will be very hard to get any script to run correctly, let alone run at all, as the latest WDM requires the latest HAgent to function correctly. I would suggest verifying that part to ensure the WDM is able to effectively communicate with the client. There is a way to use the WDM to automatically update the HAgent on the clients, though I haven't used it since I mainly use Wyse Thin OS for my clients, which updates everything when I upload a new firmware to my FTP server.

It also depends on what model of terminals you have. Back in the day, when I was an e-recycler tech, one of my jobs was to hack into such terminals, wipe them, re-image them, and get them ready for resale. Some terminals have more brains and onboard functionality than others. Back then, your average WYSE terminal was pretty dumb and had almost no processing power of their own.

But, then there were the XL models that actually ran their own, fully functioning OS (XP), and could be used as stand-alone workstations, only needing a main server for file and application support. With the addition of a USB drive, even that could be bypassed for the most part. I actually used one as a car PC for the longest time with great success before the advent of relatively cheap smartphones became the norm and made it obsolete again. Wardriving Opens a new window was a blast with it and the best part was that I could surf public internet with it in the parking lot of restaurants and not worry. If I got something icky on it by accident, reboot and keep driving with the original install.

However, if you've got a bunch of dummy terminals with just enough brains to project a picture on a screen, yeah, you're going to need a terminal server to make them run. My first step would be to research the type of terminals you have, what their capabilities are, and then go from there.

After the thin clients were well received by the market, Wyse introduced several additional models, including stand-alone (Winterm 2300), LCD monitor-integrated (Winterm 2600), and the tablet-shaped mobile Winterm 2900 and 2930 models. In 1997, Wyse introduced the first thin-client remote management software system, Wyse Remote Administrator.[14]

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There were eight major findings. First, it was found that there was an active pantetheinase in sera, negating the need for hog kidney peptidase or pigeon liver enzyme in whole blood. The second finding (related to the first) indicated that there was significant autolysis of the peptidase bond between the pantothenic acid and cysteamine moieties of coenzyme A in whole blood. The third finding was that Clarase (a monoesterase) liberated equivalent amounts of pantothenic acid in fresh whole blood when compared to alkaline phosphatase, but less in frozen blood. The optimum time for enzymatic liberation of pantothenic acid from whole blood was determined in the fourth. When using alkaline phosphatase alone, or alkaline phosphatase plus a pantetheinase, maximum liberation of pantothenic acid occurred at 4 to 5 hours. The fifth finding was that in sera, acid or base hydrolysis and boiling increased pantothenic acid liberation by approximately 100 nanograms over and above that liberated by enzyme treatment with alkaline phosphatase and hog kidney peptidase. In whole blood, both boiling and acid hydrolysis increased pantothenic acid liberation by approximately 100 nanograms; however, base hydrolysis did not yield a similar increase in pantothenic acid. In the sixth, it was found that substantial autolysis of bound forms of pantothenic acid (to liberate free pantothenic acid) occurred in fresh and frozen whole blood at 37C and 23C. The autolysis did not occur in sera or in boiled blood. The results from the seventh finding indicated that blood frozen for 15 months showed less bound (released by alkaline phosphatase plus hog kidney peptidase) pantothenic acid and a greater amount of free pantothenic acid than did fresh blood. The eighth was actually a series of findings in which it was established that a protease digestion of whole blood with trypsin, chymotrypsin, papain, or pepsin increased pantothenic acid liberation from whole blood. Of the four proteases used, trypsin was found to be optimal for pantothenic acid liberation. The pantothenic acid liberated by a pro-tease digestion was released as free pantothenic acid, and was from a different source than that liberated by alkaline phosphatase plus hog kidney peptidase. When assaying trypsin digested whole blood with both the radioimmuno and microbiological assay, the r2 between assays was 0.901. The source of the pantothenic acid which was liberated by the trypsin digestions is unknown.

Technical Abstract: Finding rare alleles in mixed genetic pools of closely related DNA is a difficult task; however, there are situations where it is necessary, especially in diagnostics. For instance, Amaranthus palmeri is an aggressive and prolific weed species that has major impact on agricultural yield and has recently been declared a prohibited noxious weed in some US states while being a native species to other. Morphological identification that distinguishes A. palmeri from other Amaranthus species is difficult in seedling plants and nigh impossible for seeds, which has led to the need for genetic testing for Amaranthus species weed seed identification in commercial seed lots. In response we have developed a method for identifying novel (species-specific) single nucleotide polymorphisms (SNPs) from Genotyping by Sequencing (GBS) data and then created an inexpensive and reliable genetic test based on those SNPs. In this paper we describe three SNP-based genetic tests for identifying A. palmeri alone or in a mixed pool of Amaranthus spp. DNA. Accuracy for all three tests is >99.7%. Furthermore, we show that all three markers are capable of reliably detecting a single A. palmeri seed in a pool of 200 Amaranthus spp. seeds. The test was validated across 20 populations of A. palmeri, along with 8 other Amaranthus species, the largest and most genetically diverse panel of Amaranthus samples to date. This research represents a marked improvement over existing commercial assays resulting in a test that is 1) accurate, 2) robust, 3) easy to interpret and 4) applicable to both leaf tissue and pools of up to 200 seeds. This assay provides a valuable tool for both seed screening and identification of suspected Palmer samples collected in the field. Our approach serves as a model for developing such markers for other difficult to identify species. ff782bc1db