Optical microscopy is an important tool for research in the life sciences and prevalent in virtually every biological laboratory. Many scientific questions especially in cellular and molecular biology, however, are challenging to answer with simple conventional microscopes but require advanced systems and expert planning of experiments. This is particularly true for conducting studies in living cells in 3D. In this course, world-leading developers of advanced next-generation live-cell microscopy instrumentation, in particular structured illumination microscopy (SIM for live cell super-resolution imaging), quantitative phase microscopy (QPM for label-free imaging of cells and cell systems), and selective plane illumination microscopy (SPIM for imaging small to large scale cell systems), will talk about the fundamentals spanning physics and computational sciences, bio-imaging application areas, and technical implementation details of their respective techniques. While the course is open for everyone to attend, students may participate in it for credits. The participants are required to present their own work revolving around development or application of microscopy techniques for life sciences. Mode of presentation is a poster (there will be a poster prize) in conjunction with a short oral "flash" presentation. The presentation is awarded with 2 ETCS.