Advanced Microscopy Tools
Advances in microscopy have led to key insights in biological structure and dynamics. By imaging and/or modifying the back focal plane of a standard microscope, we encode and decode key information that is otherwise lost in a normal measurement. This includes: 3D spatial information, spectral information, and material properties such as the refractive index.
Sonic Hedgehog Signaling
Sonic Hedgehog (SHH) signaling plays a critical role in embryonic development as well as being implicated in a variety of cancer types. Critically, key signaling components utilize a small organelle, the primary cilium, as part of the activation process. We have been using single-particle tracking to observe single pathway-transducer proteins as they explore the ciliary structure in order to study their dynamics and reveal mechanistic insights into the pathway.