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Louisville Volume EM Sample Prep Workshop
University of Louisville
Workshop Overview
Volume EM has revolutionized our understanding of cells and pathologies by combining resolution with context. However, poor staining can cause more than morphological or charging artifacts; it can also dramatically slow image acquisition.
In conjunction with the University of Louisville, Thermo Fisher Scientific will host an in-person, hands-on sample preparation workshop for volume EM. Over three days, participants will complete a basic OTO-type preparation and discuss modifications for targeted stains, immunolabeling, and CLEM.
The third day will also include sessions on image optimization in the SEM/FIB, as well as tips for improving segmentation with machine learning in Amira.
General Info
Registration Fee
$300 per participant
Registration Deadline
July 10, 2026
In-Person Workshop Dates
July 21 – 23, 2026
9am to 5pm
Registration includes workshop instruction, hands-on sessions, and training materials. Travel and accommodations are not included.
For questions, please contact:
📧 matt.joens@thermofisher.com
Who Should Apply
Graduate students
Postdoctoral researchers
Research scientists
Industry researchers
Basic familiarity with FIB-SEM, SBF-SEM or Volume EM is recommended, but applicants from diverse backgrounds and experience levels are encouraged to apply
Workshop Highlights
End-to-end FIB-SEM, SBF-SEM and Volume EM training with experts
Live data collection on a Thermo Scientific Hydra Bio P-FIB microscope
Hands-on data processing tutorials using Amira with peers and experts
Live demonstrations of Heartland Biotech's PrepMaster® 5100 Automated Workflow System will simultaneously process equivalent specimens using the rOTO protocol, providing a side-by-side comparison of traditional manual methods and state-of-the-art laboratory automation
Instructors
Matt Joens
Sr. Scientist
Thermo Fisher Scientific
Geoff Perumal
Sr. Scientist
Thermo Fisher Scientific
Julia Aebersold
Manager, MNTC-Bioimaging Core Facility
University of Louisville
Rohit Bukka
Product Applications Specialist
Thermo Fisher Scientific
Thomas E. Strader
Heartland Biotech
Working with a difficult sample?
Plant and insect samples can be challenging to process, and best practices don’t always make it into manuscripts. Reach out to us if you’re interested in bringing your own samples to the workshop. Please coordinate with Julia Aebersold at julia.aebersold@louisville.edu
Louisville, Kentucky
In Louisville, the Ohio River light seems to carry a little extra energy past the arches of the Big Four Bridge, glowing through this city known for its bourbon heritage and celebrated culinary scene.
At the University of Louisville, that same spirit of motion and ambition continues on the Belknap Campus, the university’s 274-acre main campus and home to much of its academic life.
For a Volume EM workshop, it feels like exactly the right setting: a place where Southern warmth meets discovery, where bold ideas are welcomed, and where visitors can spend the day immersed in science and spend the evening in a city full of character and history.
Belknap Academic Building, University of Louisville
201 East West Shipp Avenue
Louisville, KY 40208
Recommended Nearby Hotels:
Hilton Garden Inn Louisville Airport
Homewood Suites by Hilton Louisville Airport
Crowne Plaza Louisville Airport Expo Ctr by IHG
SpringHill Suites by Marriott Louisville Airport
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