During fermentation, the yeast Saccharomyces cerevisiae produces a broad range of aroma-active substances, which are vital for the complex flavour of beer. In order to obtain insight into the influence of high-gravity brewing and fermentation temperature on flavour formation, we analysed flavour production and the expression level of ten genes (ADH1, BAP2, BAT1, BAT2, ILV5, ATF1, ATF2, IAH1, EHT1 and EEB1) during fermentation of a lager and an ale yeast. Higher initial wort gravity increased acetate ester production, while the influence of higher fermentation temperature on aroma compound production was rather limited. In addition, there is a good correlation between flavour production and the expression level of specific genes involved in the biosynthesis of aroma compounds. We conclude that yeasts with desired amounts of esters and higher alcohols, in accordance with specific consumer preferences, may be identified based on the expression level of flavour biosynthesis genes. Moreover, these results demonstrate that the initial wort density can determine the final concentration of important volatile aroma compounds, thereby allowing beneficial adaptation of the flavour of beer.

Introduction:  To understand the impact of e-cigarette devices, flavours, nicotine levels and prices on adult e-cigarette users' choices among closed-system and open-system e-cigarettes, cigarettes and heated tobacco products (HTPs).


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Results:  On average, participants preferred non-tobacco and non-menthol flavours most, preferred open-system over closed-system e-cigarettes and preferred regular nicotine level over low nicotine level. However, the preference varied by demographics, smoking status and the primarily used e-cigarette device and flavour. The differences in preference among products/devices were larger than the difference among flavours or nicotine levels. Participants who primarily used closed-system e-cigarettes exhibited similar preferences for closed-system and open-system e-cigarettes, but those who primarily used open-system e-cigarettes preferred much more open-system over closed-system e-cigarettes. HTP was the least preferred product, much lower than cigarettes in general, but participants living in states where IQOS is being sold had similar preferences to cigarettes and HTPs.

Conclusions:  People are unlikely to switch to another product/device because of the restriction of flavour or nicotine level. If non-tobacco and non-menthol flavours were banned from open-system e-cigarettes, users may switch to menthol flavour e-cigarettes. Intervention strategies should be tailored to specific groups.

"Because coffee beans vary, color is not an especially accurate way of judging a roast. But combined with the typical roasting temperature that yields a particular shade of brown [and some other factors], color is a convenient way to categorize roasting levels." (Lokker, 2013)

DURHAM, N.C. -- A potential carcinogen that has been banned as a food additive is present in concerningly high levels in electronic cigarette liquids and smokeless tobacco products, according to a new study from Duke Health.

Among never smokers who vaped exclusively, the frequency of vaping and the use of certain popular devices and flavors were associated with the highest levels of DNA damage, according to new research from the Keck School of Medicine of USC.

The tests showed similar levels of DNA damage between vapers and smokers: 2.6 times and 2.2 times that of non-users, respectively. In terms of devices, vapers who used pods had the highest levels of DNA damage, followed by those who used mods. In terms of flavors, sweet-flavored vapes were linked to the highest levels of DNA damage, followed by mint/menthol- and fruit-flavored vapes.

Oxidation

Oxidation, particularly of white wines, was a common fault in Australian white wines 40 years ago when our table wine technology was reasonably primitive, compared to that of today. Oxidation is much less common today with the application of refrigeration, inert gas blanketing during the production and packaging operations and effective sulfur dioxide management. The oxidation flavour is due to multiple compounds including a range of aldehydes.

Acetaldehyde levels increase as wines age due to chemical oxidation of ethanol. As acetaldehyde is also an intermediate in the bacterial formation of acetic acid and under low-oxygen conditions and/or alcohol levels greater than 10% v/v, acetaldehyde tends to accumulate instead of being oxidised to acetic acid.

Factors that can influence ethyl acetate formation by yeasts include the yeast strain employed, temperature of fermentation, the amino nitrogen content of the juice and sulfur dioxide levels. As with acetic acid, native or wild yeasts such as Hansenula and Kloeckera can produce high concentrations of ethyl acetate before and during the early stages of fermentation. Ethyl acetate is also produced by acetic acid bacteria and is related to dissolved oxygen levels in the wine. It has been reported that growth of acetic acid bacteria under conditions of low oxygen tension can lead to higher levels of ethyl acetate.

Mousiness

Mousy taint is an off-flavour reminiscent of caged mice or sometimes cracker biscuit, and in sensitive individuals renders the wine undrinkable. The taint is generally perceived late on the palate or after the wine has been swallowed or expectorated and usually takes a few seconds to build. It tends to linger and leave a most obnoxious taste in the mouth for some time. If you move quickly to the next wine in a line-up, you might miss a mousy wine. Mousy taint is rarely detected by sniffing because the compounds involved are not volatile at wine pH. Note that there is considerable variation in the sensitivity between individuals to the taint.

Usually of microbial origin, most strains of lactic acid bacteria (LAB) being capable of producing the taint, particularly the heterofermentative species. These include Lactobacillus hilgardii, Lactobacillus plantarum, Lactobacillus brevis, and Oenococcus oeni (Cosello et al 2001). The yeast Dekkera/Brettanomyces may also be capable of producing mousy compounds. In addition to microbial origin, empirical observation has shown that some wines develop mousy taint when exposed to air or oxygen. The mechanism by which oxidation enhances mousy taint is currently unknown. There is no satisfactory method to remove mousy off-flavour, which is more likely to occur in wines with low concentrations of SO2 and low acidity.

Hydrogen sulfide can be produced in excess by yeast during fermentation due to the presence of elemental sulfur on grape skins (from sulfur sprays), inadequate levels of free -amino nitrogen (FAN), added SO2, a deficiency of B-complex vitamins (pantothenic acid or pyridoxine), unusually high levels of cysteine in the juice or a high concentration of metal ions. The production of H2S can also be yeast strain dependent.

In recent years, indole off-flavours have been observed during the secondary fermentation of some tank-fermented sparkling wines. More information is available in this Ask the AWRI column on indole off-flavour in sparkling wine.

Mono-, di- and trichlorophenols can easily be generated by the chemical chlorination of phenol. Chlorine-based sterilising agents, such as hypochlorite solutions, can react with traces of phenol present in materials such as plastic or fibreglass tanks or linings, phenolic-based resins, paints and fittings. Chlorophenols are also generated when wood is treated with hypochlorite solutions and are formed in the bleaching of wood pulp for paper manufacture. Sometimes wooden pallets loaded with cartons are stored near processing areas where disinfectants containing available chlorine are used. In situations such as this, chlorophenols can be generated in the cartons or pallets if they contact chlorine. It is interesting to note that Saxby (1992) indicates that the presence of dichlorophenols might indicate spillage of phenolic herbicides on the wooden floors of shipping containers. In addition, products such as fibreboard and paper made from recycled materials can often contain relatively high levels of chlorophenols, which can then contaminate food products or processing aids packaged in the fibreboard or paper (Mottram 1984).

Rotundone is one of the most potent grape-derived flavour compounds, with a sensory detection threshold value of 16 ng/L in red wine. Interestingly, approximately 20% of the population is unable to smell the compound.

From a study of wines from different varieties and vintages from various regions, the majority (62%) of the wines that contained rotundone were found to be Shiraz. Perhaps not surprisingly, above aroma-threshold levels of rotundone were often encountered in wines originating from cool climate regions. Rotundone levels were also found to vary significantly from season to season, with cooler seasons generally resulting in higher levels.

Rotundone is one of the few important grape flavour compounds found free in the grape berry, rather than coming from flavourless precursors, meaning it can be tasted in grapes. Rotundone arises very late in the ripening process and is found in grape skins rather than pulp or seeds. There is evidence from commercial vineyards that clonal differences are important, but more studies are required as Australian clonal collections are generally situated in warmer climates, with negligible rotundone present.

An additional study has shown variation even within the grape bunch, with the most sheltered or shaded part of the bunch having the highest levels of rotundone. Assessing the effect of leaf removal, bunches that were shaded gave higher rotundone concentration than those with leaves removed from the bunch zone, further indicating the important influence of light or temperature. Similarly, higher vine vigour resulted in lower rotundone concentration. 2351a5e196

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